Anti-SLC12A6/KCC3 antibody - C-terminal

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A6/KCC3 antibody - C-terminal (AB191451)

Immunohistochemical analysis of paraffin-embedded Human colorectal adenocarcinoma tissue labeling SLC12A6/KCC3 with ab191451 at 2 μg/ml. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with ab191451 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. DAB was used as the chromogen.

• WB

Supplier Data

Western blot - Anti-SLC12A6/KCC3 antibody - C-terminal (AB191451)

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with ab191451 at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1/5000 for 1.5 hour at RT.

All lanes:

Western blot - Anti-SLC12A6/KCC3 antibody - C-terminal (ab191451)

Lane 1:

Rat brain tissue lysate at 30 µg

Lane 2:

Rat C6 whole cell lysate at 30 µg

Lane 3:

Mouse brain tissue lysate at 30 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP secondary antibody at 1/5000 dilution

Observed band size: 128 kDa

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