JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB238721

Anti-SLC14A1/UTE antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal SLC14A1/UTE antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC14A1 aa 1-100.

View Alternative Names

HUT11, JK, RACH1, UT1, UTE, SLC14A1, Urea transporter 1, Solute carrier family 14 member 1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SLC14A1/UTE antibody (AB238721)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SLC14A1/UTE antibody (AB238721)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for SLC14A1/UTE (green) using ab238721 at 1/166 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC14A1/UTE antibody (AB238721)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC14A1/UTE antibody (AB238721)

Paraffin-embedded human prostate cancer tissue stained for SLC14A1/UTE using ab238721 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC14A1/UTE antibody (AB238721)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC14A1/UTE antibody (AB238721)

Paraffin-embedded human prostate tissue stained for SLC14A1/UTE using ab238721 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SLC14A1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q13336

style
left-column

Alternative Products

Primary Antibodies

AB103295

Anti-SLC14A1/UTE antibody

Western blot - Anti-SLC14A1/UTE antibody (AB103295)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB307139

Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|Advanced Validation

Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139)

  • 0
  • 0
Primary Antibodies

AB199975

Anti-Aquaporin 2 antibody [EPR21080]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (AB199975)

  • 5
  • 2
Primary Antibodies

AB181036

Anti-EAAT1 antibody [EPR12686]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (AB181036)

  • 5
  • 3
Primary Antibodies

AB3259

Anti-SREBP1 antibody [2A4]

Trial Size

Western blot - Anti-SREBP1 antibody [2A4] (AB3259)

  • 4
  • 12
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC14A1 also known as UTE is a protein that functions as a urea transporter. It facilitates the movement of urea across cell membranes specifically in the kidney and erythrocytes. It has a molecular weight of approximately 50 kDa. The expression of SLC14A1 is notable in the renal medulla and red blood cells where it plays a role in maintaining the body's urea balance. This transporter contributes to urine concentration and detoxification processes by efficiently regulating urea levels within different tissues and body fluids.
Biological function summary

The function of SLC14A1 in transporting urea is important for nitrogen waste removal. It operates within renal epithelial cells to assist with the reabsorption of urea from urine back into the bloodstream contributing to water conservation and nitrogen excretion. SLC14A1 is not known to be part of a larger protein complex but interacts with other renal transporters to enhance its role in concentrating urine. This transport activity helps in creating the osmotic gradient necessary for the kidney's urine concentrating mechanism.

Pathways

SLC14A1 integrates into the renal system's water and urea reabsorption processes. It is pivotal in the urea cycle and the renal concentrating mechanism pathways. Its functionality is closely linked with aquaporin channels which are responsible for water reabsorption in the kidney. These pathways coordinate to regulate body fluid and electrolyte balance establishing a direct relationship between SLC14A1 and aquaporins to optimize urea transport and water reabsorption throughout the physiological system.

SLC14A1's impaired function is linked to urea transport dysfunctions such as urinary concentrating deficiency which can lead to conditions like nephrogenic diabetes insipidus. Defects or mutations in its gene can disrupt its transporter activity and affect the body's ability to concentrate urine leading to polyuria and dehydration. In certain scenarios altered activity of SLC14A1 might also interact with conditions related to anemia where urea transport in erythrocytes plays a role in maintaining red blood cell function and stability.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Mediates the transport of urea driven by a concentration gradient across the cell membrane of erythrocytes (PubMed : 10514515, PubMed : 7797558, PubMed : 7989337, PubMed : 8997401). Also mediates the transport of urea across the cell membrane of the renal inner medullary collecting duct which is critical to the urinary concentrating mechanism (By similarity). Facilitates water transport in erythrocytes (By similarity).
See full target information SLC14A1
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 43:208 PubMed39061061

2024

SLC14A1 and TGF-β signaling: a feedback loop driving EMT and colorectal cancer metachronous liver metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yixun Zhang,Yumeng Yang,Xuan Qi,Peng Cui,Yi Kang,Haiyi Liu,Zhigang Wei,Haibo Wang

Aging 14:9679-9698 PubMed36462498

2022

SKA1 promotes tumor metastasis via SAFB-mediated transcription repression of DUSP6 in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Pu,Jing Han,Mengmeng Zhang,Mengxue Liu,Gulnazar Abdusamat,Huibin Liu
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com