AB156589

Anti-SLC14A2 antibody [EPR8620]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal SLC14A2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

HUT2, UT2, SLC14A2, Urea transporter 2, Solute carrier family 14 member 2

2 Images

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Immunoprecipitation - Anti-SLC14A2 antibody [EPR8620] (AB156589)

Detection of SLC14A2 by Western Blot of Immunprecipitate.
293T cell lysate pellet, immunoprecipitated using ab156589 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-SLC14A2 antibody [EPR8620] (ab156589)

Predicted band size: 101 kDa

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Western blot - Anti-SLC14A2 antibody [EPR8620] (AB156589)

All lanes:

Western blot - Anti-SLC14A2 antibody [EPR8620] (ab156589) at 1/1000 dilution

Lane 1:

K562 lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Lane 3:

293T lysate at 10 µg

Lane 4:

TF1 lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 101 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8620

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC14A2 also known as urea transporter B (UT-B) functions as a urea transporter protein. Its mass is approximately 102 kDa. SLC14A2 is expressed in several tissues with high levels found in the kidneys bladder and red blood cells. The protein facilitates the reabsorption of urea in the renal medulla playing an essential role in the urinary concentrating mechanism.

Biological function summary

SLC14A2 contributes to the regulation of urea transport across cell membranes to maintain nitrogen balance. It does not function as part of a larger protein complex but works in conjunction with other transporters to modulate urea concentrations. This process is vital in organisms that require efficient excretion of nitrogen products such as mammals.

Pathways

SLC14A2 functions within the renal medullar urea recycling and excretion pathway. It acts together with aquaporins particularly AQP1 and AQP3 to regulate water reabsorption and enhance urinary concentration. Additionally SLC14A2 plays a part in the urea cycle linking it to nitrogen metabolism pathways that involve enzymes such as carbamoyl phosphate synthetase 1.

Mutations or dysfunctions in SLC14A2 can lead to nephrogenic diabetes insipidus due to impaired water reabsorption. This condition relates to defects in the urinary concentrating ability of the kidneys often connected to altered aquaporin function. Changes in SLC14A2 activity have also been associated with disorders of electrolyte imbalance impacting proteins involved in transport regulation like SLC26A4.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform 1. Mediates the transport of urea driven by a concentration gradient across the cell membrane of the renal inner medullary collecting duct which is critical to the urinary concentrating mechanism.. Isoform 2. Mediates the transport of urea driven by a concentration gradient across the cell membrane of the kidney inner medullary collecting duct which is critical to the urinary concentrating mechanism.

See full target information SLC14A2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Cell physiology 317:C31-C38 PubMed31067085

2019

Emerging new role of NFAT5 in inducible nitric oxide synthase in response to hypoxia in mouse embryonic fibroblast cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yair Serman,Rodrigo A Fuentealba,Consuelo Pasten,Jocelyn Rocco,Ben C B Ko,Flavio Carrión,Carlos E Irarrázabal

Oncology reports 36:3131-3138 PubMed27748938

2016

Comparative proteomic profiling identifies potential prognostic factors for human clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Sun,Hongwei Zhang,Longhua Luo,Kezhao Zhong,Yushui Ma,Linlin Fan,Da Fu,Lijuan Wan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLC14A2 antibody [EPR8620]

Immunoprecipitation - Anti-SLC14A2 antibody [EPR8620] (AB156589)

Western blot - Anti-SLC14A2 antibody [EPR8620] (AB156589)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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