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AB249277

Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free

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Rabbit Recombinant Monoclonal SLC14A2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

HUT2, UT2, SLC14A2, Urea transporter 2, Solute carrier family 14 member 2

2 Images
Immunoprecipitation - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (AB249277)
  • IP

Unknown

Immunoprecipitation - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (AB249277)

This data was developed using ab156589, the same antibody clone in a different buffer formulation.Detection of SLC14A2 by Western Blot of Immunprecipitate.
293T cell lysate pellet, immunoprecipitated using ab156589 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (ab249277)

Predicted band size: 101 kDa

false

Western blot - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (AB249277)
  • WB

Unknown

Western blot - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (AB249277)

This data was developed using ab156589, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SLC14A2 antibody [EPR8620] (<a href='/en-us/products/primary-antibodies/slc14a2-antibody-epr8620-ab156589'>ab156589</a>) at 1/1000 dilution

Lane 1:

K562 lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Lane 3:

293T lysate at 10 µg

Lane 4:

TF1 lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 101 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8620

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab249277 is the carrier-free version of ab156589.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC14A2 also known as urea transporter B (UT-B) functions as a urea transporter protein. Its mass is approximately 102 kDa. SLC14A2 is expressed in several tissues with high levels found in the kidneys bladder and red blood cells. The protein facilitates the reabsorption of urea in the renal medulla playing an essential role in the urinary concentrating mechanism.
Biological function summary

SLC14A2 contributes to the regulation of urea transport across cell membranes to maintain nitrogen balance. It does not function as part of a larger protein complex but works in conjunction with other transporters to modulate urea concentrations. This process is vital in organisms that require efficient excretion of nitrogen products such as mammals.

Pathways

SLC14A2 functions within the renal medullar urea recycling and excretion pathway. It acts together with aquaporins particularly AQP1 and AQP3 to regulate water reabsorption and enhance urinary concentration. Additionally SLC14A2 plays a part in the urea cycle linking it to nitrogen metabolism pathways that involve enzymes such as carbamoyl phosphate synthetase 1.

Mutations or dysfunctions in SLC14A2 can lead to nephrogenic diabetes insipidus due to impaired water reabsorption. This condition relates to defects in the urinary concentrating ability of the kidneys often connected to altered aquaporin function. Changes in SLC14A2 activity have also been associated with disorders of electrolyte imbalance impacting proteins involved in transport regulation like SLC26A4.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. Mediates the transport of urea driven by a concentration gradient across the cell membrane of the renal inner medullary collecting duct which is critical to the urinary concentrating mechanism.. Isoform 2. Mediates the transport of urea driven by a concentration gradient across the cell membrane of the kidney inner medullary collecting duct which is critical to the urinary concentrating mechanism.
See full target information SLC14A2
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Product promise

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For full details, please see our Terms & Conditions

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