Rabbit Recombinant Monoclonal SLC14A2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Images
![Western blot - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (AB249277), expandable thumbnail](https://content.abcam.com/products/images/slc14a2-antibody-epr8620-bsa-and-azide-free-ab249277--western-blot-img115082.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (AB249277)")
![Immunoprecipitation - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (AB249277), expandable thumbnail](https://content.abcam.com/products/images/slc14a2-antibody-epr8620-bsa-and-azide-free-ab249277--immunoprecipitation-img76759.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (AB249277)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Isoform 1. Mediates the transport of urea driven by a concentration gradient across the cell membrane of the renal inner medullary collecting duct which is critical to the urinary concentrating mechanism. Isoform 2. Mediates the transport of urea driven by a concentration gradient across the cell membrane of the kidney inner medullary collecting duct which is critical to the urinary concentrating mechanism.
Alternative names
HUT2, UT2, SLC14A2, Urea transporter 2, Solute carrier family 14 member 2
Recommended products
Rabbit Recombinant Monoclonal SLC14A2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8620
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249277 is the carrier-free version of Anti-SLC14A2 antibody [EPR8620] ab156589.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SLC14A2 also known as urea transporter B (UT-B) functions as a urea transporter protein. Its mass is approximately 102 kDa. SLC14A2 is expressed in several tissues with high levels found in the kidneys bladder and red blood cells. The protein facilitates the reabsorption of urea in the renal medulla playing an essential role in the urinary concentrating mechanism.
- Biological function summary
SLC14A2 contributes to the regulation of urea transport across cell membranes to maintain nitrogen balance. It does not function as part of a larger protein complex but works in conjunction with other transporters to modulate urea concentrations. This process is vital in organisms that require efficient excretion of nitrogen products such as mammals.
- Pathways
SLC14A2 functions within the renal medullar urea recycling and excretion pathway. It acts together with aquaporins particularly AQP1 and AQP3 to regulate water reabsorption and enhance urinary concentration. Additionally SLC14A2 plays a part in the urea cycle linking it to nitrogen metabolism pathways that involve enzymes such as carbamoyl phosphate synthetase 1.
- Associated diseases and disorders
Mutations or dysfunctions in SLC14A2 can lead to nephrogenic diabetes insipidus due to impaired water reabsorption. This condition relates to defects in the urinary concentrating ability of the kidneys often connected to altered aquaporin function. Changes in SLC14A2 activity have also been associated with disorders of electrolyte imbalance impacting proteins involved in transport regulation like SLC26A4.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (ab249277), expandable thumbnail](https://content.abcam.com/products/images/slc14a2-antibody-epr8620-bsa-and-azide-free-ab249277--western-blot-img115082.jpg "Western blot - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (ab249277)")
Western blot - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (ab249277)
This data was developed using Anti-SLC14A2 antibody [EPR8620] ab156589, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SLC14A2 antibody [EPR8620] (Anti-SLC14A2 antibody [EPR8620] ab156589) at 1/1000 dilution
Lane 1: K562 lysate at 10 µg
Lane 2: Human fetal kidney lysate at 10 µg
Lane 3: 293T lysate at 10 µg
Lane 4: TF1 lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 101 kDa
![Immunoprecipitation - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (ab249277), expandable thumbnail](https://content.abcam.com/products/images/slc14a2-antibody-epr8620-bsa-and-azide-free-ab249277--immunoprecipitation-img76759.jpg "Immunoprecipitation - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (ab249277)")
Immunoprecipitation - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (ab249277)
This data was developed using Anti-SLC14A2 antibody [EPR8620] ab156589, the same antibody clone in a different buffer formulation.Detection of SLC14A2 by Western Blot of Immunprecipitate.
293T cell lysate pellet, immunoprecipitated using Anti-SLC14A2 antibody [EPR8620] ab156589 at 1/10 dilution.All lanes: Immunoprecipitation - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (ab249277)
Predicted band size: 101 kDa
Downloads
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