AB78020

Anti-SLC15A1/PEPT1 antibody

Name: Anti-SLC15A1/PEPT1 antibody (ab78020) | Abcam
Brand: Abcam Limited
SKU: AB78020

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(5 Publications)

Rabbit Polyclonal SLC15A1/PEPT1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications.

View Alternative Names

PEPT1, SLC15A1, Solute carrier family 15 member 1, Intestinal H(+)/peptide cotransporter, Peptide transporter 1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC15A1/PEPT1 antibody (AB78020)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC15A1/PEPT1 antibody (AB78020)

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human brain cerebellum tissue using 10µg/ml ab78017.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC15A1 also known as PEPT1 is a protein that functions as a proton-coupled oligopeptide transporter. It facilitates the uptake of di- and tripeptides from the intestinal lumen which is essential for nutrient absorption and peptide-based drug delivery. The molecular weight of PEPT1 is approximately 78 kDa. The expression of PEPT1 is mainly in the brush border membrane of small intestine epithelial cells playing a significant role in the digestive system's initial phase of protein digestion.

Biological function summary

PEPT1 mediates the cellular uptake of nutrient peptides and peptidomimetic drugs. PEPT1 functions as a transporter that is not part of a larger protein complex but interacts closely with various solute carrier proteins. Its activity helps regulate amino acid availability within the intestinal cells influencing local metabolic pathways and systemic amino acid levels.

Pathways

PEPT1 is integrally involved in the protein digestion and absorption pathway influencing the systemic availability of dietary peptides and drugs. This protein also connects with other transporters such as SLC15A2 (PEPT2) in related pathways to manage di- and tripeptide absorption and reabsorption. Together these transporters ensure efficient nutrient acquisition and influence pharmacokinetics for orally administered peptide-based medications.

Dysregulation of PEPT1 expression or function associates with conditions such as inflammatory bowel disease (IBD) and malabsorption syndromes. Variants in the SLC15A1 gene might affect PEPT1 altering its transport efficiency and contributing to these gastrointestinal disorders. Additionally changes in PEPT1 activity could influence the bioavailability of peptide-based drugs affecting therapeutic outcomes for diseases requiring such treatments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Electrogenic proton-coupled amino-acid transporter that transports oligopeptides of 2 to 4 amino acids with a preference for dipeptides. Transports neutral and monovalently charged peptides with a proton to peptide stoichiometry of 1 : 1 or 2 : 1 (By similarity) (PubMed : 15521010, PubMed : 18367661, PubMed : 19685173, PubMed : 26320580, PubMed : 7896779, PubMed : 8914574, PubMed : 9835627). Primarily responsible for the absorption of dietary di- and tripeptides from the small intestinal lumen (By similarity). Mediates transepithelial transport of muramyl and N-formylated bacterial dipeptides contributing to recognition of pathogenic bacteria by the mucosal immune system (PubMed : 15521010, PubMed : 9835627).

See full target information SLC15A1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell stem cell 29:101-115.e10 PubMed34582804

2021

SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Gu,Hua Wang,Xiaofeng Huang,Judith Kraiczy,Pratik N P Singh,Charles Ng,Sezin Dagdeviren,Sean Houghton,Oscar Pellon-Cardenas,Ying Lan,Yaohui Nie,Jiaoyue Zhang,Kushal K Banerjee,Emily J Onufer,Brad W Warner,Jason Spence,Ellen Scherl,Shahin Rafii,Richard T Lee,Michael P Verzi,David Redmond,Randy Longman,Kristian Helin,Ramesh A Shivdasani,Qiao Zhou

Cell death & disease 12:532 PubMed34031358

2021

Epigenetic regulation of intestinal peptide transporter PEPT1 as a potential strategy for colorectal cancer sensitization.

Applications

Species

Human

Yanhong Wang,Jiaqi Wang,Lingrong Yang,Liqing Qiu,Yuhui Hua,Shixiu Wu,Su Zeng,Lushan Yu,Xiaoli Zheng

Journal of animal physiology and animal nutrition 103:1610-1618 PubMed31106911

2019

Effects of methionine partially replaced by methionyl-methionine dipeptide on intestinal function in methionine-deficient pregnant mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Chen,Caihong Wang,Feng-Qi Zhao,Jianxin Liu,Hongyun Liu

Journal of animal science and biotechnology 9:7 PubMed29387385

2018

Functional characterization of oligopeptide transporter 1 of dairy cows.

Applications

Unspecified application

Species

Unspecified reactive species

Qingbiao Xu,Zhixuan Liu,Hongyun Liu,Fengqi Zhao,Xinbei Huang,Yueming Wu,Jianxin Liu

Oncology letters 14:4158-4166 PubMed28943923

2017

Specific expression of proton-coupled oligopeptide transporter 1 in primary hepatocarcinoma-a novel strategy for tumor-targeted therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yanxia Gong,Jie Zhang,Xiang Wu,Tao Wang,Jia Zhao,Zhi Yao,Qingyu Zhang,Xi Liu,Xu Jian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLC15A1/PEPT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC15A1/PEPT1 antibody (AB78020)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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