Anti-SLC15A4/PHT1 antibody [EPR26040-123]

• WB

Supplier Data

Western blot - Anti-SLC15A4/PHT1 antibody [EPR26040-123] (AB324433)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 33560415; PMID : 37527038).

Samples are non-boiled as boiling may cause protein aggregation.

PHT1 is a glycoprotein of approximately 50-100 kDa and detected as 37-50 kDa band after treated with Deglycosylation Peptide : N-glycosidase F (PNGase F).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SLC15A4/PHT1 antibody [EPR26040-123] (ab324433) at 1/1000 dilution

Lane 1:

Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 40 µg

Lane 2:

THP-1 treated with Peptide:N-glycosidase F (PNGase F) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-SLC15A4/PHT1 antibody [EPR26040-123] (AB324433)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : colon (PMID : 11741232, PMID : 11741260)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 33560415; PMID : 37527038).

This blot of lanes1-4 and lane 7 was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

Samples are non-boiled as boiling may cause protein aggregation.

The identity of the higher MW band at approximately 300 kDa in lane 7 is unknown.

The identity of the lower MW band at approximately 40 kDa in lane 3 is unknown.

Lanes 1-4 and lane 7 are incubated with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 and lanes 5-6 are incubated with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-4 : 48 seconds; Lanes 6-7 : 180 seconds

All lanes:

Western blot - Anti-SLC15A4/PHT1 antibody [EPR26040-123] (ab324433) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 48 µg

Lane 2:

Human liver tissue lysate at 48 µg

Lane 3:

Human tonsil tissue lysate at 48 µg

Lane 4:

Human colon tissue lysate at 48 µg

Lane 5:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 48 µg

Lane 6:

A-673 (human muscle Ewings sarcoma cell line) whole cell lysate at 48 µg

Lane 7:

Human skeletal muscle tissue lysate at 48 µg

Secondary

Lanes 1, 2, 3, 4 and 7:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Lanes 5 - 6:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

true

• WB

Supplier Data

Western blot - Anti-SLC15A4/PHT1 antibody [EPR26040-123] (AB324433)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 33560415; PMID : 37527038).

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

Samples are non-boiled as boiling may cause protein aggregation.

The identity of the lower MW band at approximately 40 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SLC15A4/PHT1 antibody [EPR26040-123] (ab324433) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 40 µg

Lane 2:

293T transfected with siRNA specifically targeting PHT1 whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

true

Exposure time: 103s

• WB

Supplier Data

Western blot - Anti-SLC15A4/PHT1 antibody [EPR26040-123] (AB324433)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 33560415; PMID : 37527038).

Samples are non-boiled as boiling may cause protein aggregation.

The identity of the lower MW band at approximately 40 kDa in lane 1 and lane 3 is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SLC15A4/PHT1 antibody [EPR26040-123] (ab324433) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole lysate at 40 µg

Lane 2:

293T mitochondrial fraction at 40 µg

Lane 3:

293T non-mitochondrial fraction at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 136s