AB234728

Anti-SLC16A3/MCT 4 antibody

Name: Anti-SLC16A3/MCT 4 antibody (ab234728) | Abcam
Brand: Abcam Limited
SKU: AB234728
Price: 515 USD
Availability: InStock

Be the first to review this product! Submit a review

(8 Publications)

Rabbit Polyclonal SLC16A3/MCT 4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC16A3 aa 400 to C-terminus.

View Alternative Names

MCT3, MCT4, SLC16A3, Monocarboxylate transporter 4, MCT 4, Solute carrier family 16 member 3

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-SLC16A3/MCT 4 antibody (AB234728)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SLC16A3/MCT 4 antibody (AB234728)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SLC16A3/MCT 4 using ab234728 at a dilution of 1/100 in ICC/IF. Secondary antibody is an Alexa-Fluor^® 488-conjugated Goat Anti-Rabbit IgG (H+L).

Supplier Data

Western blot - Anti-SLC16A3/MCT 4 antibody (AB234728)

All lanes:

Western blot - Anti-SLC16A3/MCT 4 antibody (ab234728) at 1/2500 dilution

Lane 1:

MCF-7 whole cell lysate

Lane 2:

HeLa whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/5000 dilution

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SLC16A3 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15427

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC16A3 also known as MCT4 or MCT-4 is a member of the monocarboxylate transporter family. It is responsible for the transport of lactate and other monocarboxylates across cell membranes. Typically the molecular weight of MCT4 is approximately 45-50 kDa. This transporter is most abundant in tissues with high glycolytic activity including muscle and certain types of tumors where it facilitates the export of lactate produced during anaerobic metabolism to maintain intracellular pH balance and energy production efficiency.

Biological function summary

The transport function of SLC16A3/MCT4 is important for maintaining the energy equilibrium in cells that rely heavily on glycolysis. MCT4 does not function independently; it associates with ancillary proteins like CD147/Basigin which is essential for its correct localization to the plasma membrane. This association enhances the transport efficiency of lactate making MCT4 an important player in the lactate shuttle system a mechanism that allows for effective energy utilization in muscle fibers and tumor cells and significantly contributes to the regulation of the tissue microenvironment.

Pathways

SLC16A3/MCT4 plays a significant role in metabolic pathways particularly the glycolytic pathway by facilitating lactate efflux. The transporter is also implicated in the Cori cycle where lactate is shuttled from muscles to the liver for gluconeogenesis. Its functions overlap with those of other monocarboxylate transporters such as MCT1 which generally handles the import of lactate in oxidative tissues. Together these transporters maintain homeostasis of lactate levels in various tissues preventing lactate accumulation.

Alterations in SLC16A3/MCT4 expression or function are associated with cancer and anaerobic exercise intolerance. In cancer overexpression of MCT4 correlates with aggressive tumor behavior due to its role in lactate export aiding in the acidic tumor microenvironment development which favors cancer cell survival and invasion. Additionally interactions with proteins like CD147 are thought to modulate tumor progression. In exercise intolerance impaired lactate transport leads to muscle fatigue and reduced exercise capacity highlighting the critical role MCT4 plays in normal metabolic responses during intense physical activity.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Proton-dependent transporter of monocarboxylates such as L-lactate and pyruvate (PubMed : 11101640, PubMed : 23935841, PubMed : 31719150). Plays a predominant role in L-lactate efflux from highly glycolytic cells (By similarity).

See full target information SLC16A3

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:69 PubMed39810250

2025

Lactate accumulation promotes immunosuppression and fibrotic transformation of bone marrow microenvironment in myelofibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mariarita Spampinato,Cesarina Giallongo,Sebastiano Giallongo,Enrico La Spina,Andrea Duminuco,Lucia Longhitano,Rosario Caltabiano,Lucia Salvatorelli,Giuseppe Broggi,Elisabetta P Pricoco,Vittorio Del Fabro,Ilaria Dulcamare,Antonio Massimo DI Mauro,Alessandra Romano,Francesco Di Raimondo,Giovanni Li Volti,Giuseppe A Palumbo,Daniele Tibullo

BMC cancer 24:508 PubMed39103807

2024

Identification and validation of genes associated with prognosis of cisplatin-resistant ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dajiang Liu,Ruiyun Li,Yidan Wang,Dan Li,Leilei Li

Cell proliferation 56:e13388 PubMed36794373

2023

Lactate trafficking inhibition restores sensitivity to proteasome inhibitors and orchestrates immuno-microenvironment in multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro Barbato,Cesarina Giallongo,Sebastiano Giallongo,Alessandra Romano,Grazia Scandura,Saoca Concetta,Tatiana Zuppelli,Marco Lolicato,Giacomo Lazzarino,Nunziatina Parrinello,Vittorio Del Fabro,Paolo Fontana,M'hammed Aguennoz,Giovanni Li Volti,Giuseppe A Palumbo,Francesco Di Raimondo,Daniele Tibullo

Molecular cancer research : MCR 21:458-471 PubMed36735350

2023

Bone Marrow Mesenchymal Stem Cells Induce Metabolic Plasticity in Estrogen Receptor-Positive Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Johanna M Buschhaus,Shrila Rajendran,Siyi Chen,Bryan L Wharram,Avinash S Bevoor,Alyssa C Cutter,Brock A Humphries,Tanner H Robison,Alex P Farfel,Gary D Luker

Journal of translational medicine 21:53 PubMed36709284

2023

Upregulation of HMGB1 in tumor-associated macrophages induced by tumor cell-derived lactate further promotes colorectal cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyi Gao,Shiqi Zhou,Zhaofu Qin,Dechuan Li,Yuping Zhu,Dening Ma

Analytical cellular pathology (Amsterdam) 2022:6718566 PubMed36340268

2022

Monocarboxylate Transporters: Role and Regulation in Corneal Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Pawan Shrestha,Amy E Whelchel,Sarah E Nicholas,Wentao Liang,Jian-Xing Ma,Dimitrios Karamichos

Scientific reports 12:6263 PubMed35428816

2022

Intracellular pH affects mitochondrial homeostasis in cultured human corneal endothelial cells prepared for cell injection therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Hideto Deguchi,Tomoko Yamashita,Nao Hiramoto,Yohei Otsuki,Atsushi Mukai,Morio Ueno,Chie Sotozono,Shigeru Kinoshita,Junji Hamuro

Frontiers in genetics 11:566918 PubMed33424916

2020

Transcriptomic Analysis of Glycolysis-Related Genes Reveals an Independent Signature of Bladder Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zezhong Mou,Chen Yang,Zheyu Zhang,Siqi Wu,Chenyang Xu,Zhang Cheng,Xiyu Dai,Xinan Chen,Yuxi Ou,Haowen Jiang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLC16A3/MCT 4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SLC16A3/MCT 4 antibody (AB234728)

Western blot - Anti-SLC16A3/MCT 4 antibody (AB234728)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise