Anti-SLC16A3/MCT 4 antibody [EPR28177-30] 20 ul size (ab308528)

Rabbit Recombinant Monoclonal SLC16A3/MCT 4 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (AB308528), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF	Flow Cyt (Intra)	IHC-Fr	IP
Human	Tested	Tested	Tested	Tested	Expected	Not recommended
Mouse	Tested	Tested	Expected	Expected	Tested	Not recommended
Rat	Tested	Not recommended	Expected	Expected	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -
Species Rat	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Target data

See full target information SLC16A3

Function

Proton-dependent transporter of monocarboxylates such as L-lactate and pyruvate (PubMed:11101640, PubMed:23935841, PubMed:31719150). Plays a predominant role in L-lactate efflux from highly glycolytic cells (By similarity).

Alternative names

MCT3, MCT4, SLC16A3, Monocarboxylate transporter 4, MCT 4, Solute carrier family 16 member 3

Recommended products

Rabbit Recombinant Monoclonal SLC16A3/MCT 4 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR28177-30
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SLC16A3 also known as MCT4 or MCT-4 is a member of the monocarboxylate transporter family. It is responsible for the transport of lactate and other monocarboxylates across cell membranes. Typically the molecular weight of MCT4 is approximately 45-50 kDa. This transporter is most abundant in tissues with high glycolytic activity including muscle and certain types of tumors where it facilitates the export of lactate produced during anaerobic metabolism to maintain intracellular pH balance and energy production efficiency.

Biological function summary

The transport function of SLC16A3/MCT4 is important for maintaining the energy equilibrium in cells that rely heavily on glycolysis. MCT4 does not function independently; it associates with ancillary proteins like CD147/Basigin which is essential for its correct localization to the plasma membrane. This association enhances the transport efficiency of lactate making MCT4 an important player in the lactate shuttle system a mechanism that allows for effective energy utilization in muscle fibers and tumor cells and significantly contributes to the regulation of the tissue microenvironment.

Pathways

SLC16A3/MCT4 plays a significant role in metabolic pathways particularly the glycolytic pathway by facilitating lactate efflux. The transporter is also implicated in the Cori cycle where lactate is shuttled from muscles to the liver for gluconeogenesis. Its functions overlap with those of other monocarboxylate transporters such as MCT1 which generally handles the import of lactate in oxidative tissues. Together these transporters maintain homeostasis of lactate levels in various tissues preventing lactate accumulation.

Associated diseases and disorders

Alterations in SLC16A3/MCT4 expression or function are associated with cancer and anaerobic exercise intolerance. In cancer overexpression of MCT4 correlates with aggressive tumor behavior due to its role in lactate export aiding in the acidic tumor microenvironment development which favors cancer cell survival and invasion. Additionally interactions with proteins like CD147 are thought to modulate tumor progression. In exercise intolerance impaired lactate transport leads to muscle fatigue and reduced exercise capacity highlighting the critical role MCT4 plays in normal metabolic responses during intense physical activity.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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12 product images

Western blot - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (ab308528)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregation.

Exposure times: Lane 1: 103 seconds, Lane 2: 180 seconds.
All lanes: Western blot - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (ab308528) at 1/1000 dilution
Lane 1: Mouse skeletal muscle tissue lysate at 20 µg
Lane 2: Rat skeletal muscle tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 49 kDa
Observed band size: 43 kDa
Western blot - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (ab308528)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Negative control: K-562 (PMID:25957999).

Low expression: MCF7 and T-47D (PMID:35023320).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

Exposure time: 80 seconds.
All lanes: Western blot - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (ab308528) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate, at 20 µg
Lane 2: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate, at 20 µg
Lane 4: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
Lane 5: T-47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 20 µg
Lane 6: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 49 kDa
Observed band size: 43 kDa
Exposure time: 80s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (ab308528)
Immunohistochemical analysis of paraffin-embedded Mouse pancreatic tum tissue labelling SLC16A3/MCT 4 with ab308528 at 1/100 (5.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse pancreatic cancer.The section was incubated with ab308528 for 30 mins at room temperature. The
immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (ab308528)
Immunohistochemical analysis of paraffin-embedded Human pancreatic can tissue labelling SLC16A3/MCT 4 with ab308528 at 1/100 (5.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human pancreatic cancer (PMID: 29170050).The section was incubated with ab308528 for 30 mins at room temperature. The
immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (ab308528)
Immunohistochemical analysis of paraffin-embedded Human lung tissue labelling SLC16A3/MCT 4 with ab308528 at 1/100 (5.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on macrophages of human lung.The section was incubated with ab308528 for 30 mins at room temperature. The
immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (ab308528)
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling SLC16A3/MCT 4 with ab308528 at 1/100 (5.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human lung carcinoma.The section was incubated with ab308528 for 30 mins at room temperature. The
immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (ab308528)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling SLC16A3/MCT 4 with ab308528 at 1/100 (5.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression: No staining on normal human cerebrum (PMID: 23258846).The section was incubated with ab308528 for 30 mins at room temperature. The
immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (ab308528)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skeletal muscle (fresh) tissue labeling SLC16A3/MCT 4 with ab308528 at 1/100 (5.1 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on rat skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab308528 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (ab308528)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh) tissue labeling SLC16A3/MCT 4 with ab308528 at 1/100 (5.1 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab308528 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Flow Cytometry (Intracellular) - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (ab308528)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K-562(human chronic myelogenous leukemia lymphoblast, Left) / PC-3 (human prostate adenocarcinoma epithelial cell, Right) cells labelling SLC16A3/MCT 4 with ab308528 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control: K-562(PMID:25957999).
Immunocytochemistry/ Immunofluorescence - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (ab308528)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labelling SLC16A3/MCT 4 with ab308528 at 1/100 (5.1 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous staining in PC-3 cells.Negative control: K-562 (PMID: 25957999);Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (ab308528)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labelling SLC16A3/MCT 4 with ab308528 at 1/100 (5.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse skeletal muscle (PMID: 18003750).The section was incubated with ab308528 for 30 mins at room temperature. The
immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins