Anti-SLC16A3/MCT 4 antibody [EPR28177-30]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (AB308528)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labelling SLC16A3/MCT 4 with ab308528 at 1/100 (5.1 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous staining in PC-3 cells.Negative control : K-562 (PMID : 25957999);Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (AB308528)

Immunohistochemical analysis of paraffin-embedded Human pancreatic can tissue labelling SLC16A3/MCT 4 with ab308528 at 1/100 (5.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human pancreatic cancer (PMID : 29170050).The section was incubated with ab308528 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (AB308528)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labelling SLC16A3/MCT 4 with ab308528 at 1/100 (5.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on macrophages of human lung.The section was incubated with ab308528 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (AB308528)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling SLC16A3/MCT 4 with ab308528 at 1/100 (5.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human lung carcinoma.The section was incubated with ab308528 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (AB308528)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling SLC16A3/MCT 4 with ab308528 at 1/100 (5.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression : No staining on normal human cerebrum (PMID : 23258846).The section was incubated with ab308528 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (AB308528)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K-562(human chronic myelogenous leukemia lymphoblast, Left) / PC-3 (human prostate adenocarcinoma epithelial cell, Right) cells labelling SLC16A3/MCT 4 with ab308528 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : K-562(PMID : 25957999).

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (AB308528)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skeletal muscle (fresh) tissue labeling SLC16A3/MCT 4 with ab308528 at 1/100 (5.1 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on rat skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab308528 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (AB308528)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labelling SLC16A3/MCT 4 with ab308528 at 1/100 (5.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse skeletal muscle (PMID : 18003750).The section was incubated with ab308528 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (AB308528)

Immunohistochemical analysis of paraffin-embedded Mouse pancreatic tum tissue labelling SLC16A3/MCT 4 with ab308528 at 1/100 (5.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse pancreatic cancer.The section was incubated with ab308528 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (AB308528)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh) tissue labeling SLC16A3/MCT 4 with ab308528 at 1/100 (5.1 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab308528 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

• WB

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Western blot - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (AB308528)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : K-562 (PMID : 25957999). Low expression : MCF7 and T-47D (PMID : 35023320). Samples are non-boiled as boiling may cause protein aggregation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 80 seconds.

All lanes:

Western blot - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (ab308528) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate, at 20 µg

Lane 2:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate, at 20 µg

Lane 4:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Lane 5:

T-47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 20 µg

Lane 6:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 49 kDa

Observed band size: 43 kDa

false

Exposure time: 80s

• WB

Supplier Data

Western blot - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (AB308528)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Samples are non-boiled as boiling may cause protein aggregation. Exposure times : Lane 1 : 103 seconds, Lane 2 : 180 seconds.

All lanes:

Western blot - Anti-SLC16A3/MCT 4 antibody [EPR28177-30] (ab308528) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 2:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 49 kDa

Observed band size: 43 kDa

false