Rabbit Recombinant Monoclonal SLC17A5 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-SLC17A5 antibody [EPR27951-4] (AB314223), expandable thumbnail](https://content.abcam.com/products/images/slc17a5-antibody-epr27951-4-ab314223--western-blot-img421041.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SLC17A5 antibody [EPR27951-4] (AB314223)")
![Western blot - Anti-SLC17A5 antibody [EPR27951-4] (AB314223), expandable thumbnail](https://content.abcam.com/products/images/slc17a5-antibody-epr27951-4-ab314223--western-blot-img421043.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SLC17A5 antibody [EPR27951-4] (AB314223)")
![Western blot - Anti-SLC17A5 antibody [EPR27951-4] (AB314223), expandable thumbnail](https://content.abcam.com/products/images/slc17a5-antibody-epr27951-4-ab314223--western-blot-img421042.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SLC17A5 antibody [EPR27951-4] (AB314223)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | IP | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Multifunctional anion transporter that operates via two distinct transport mechanisms, namely proton-coupled anion cotransport and membrane potential-dependent anion transport (PubMed:15510212, PubMed:21781115, PubMed:22778404, PubMed:23889254). Electroneutral proton-coupled acidic monosaccharide symporter, with a sugar to proton stoichiometry of 1:1. Exports glucuronic acid and free sialic acid derived from sialoglycoconjugate degradation out of lysosomes, driven by outwardly directed lysosomal pH gradient. May regulate lysosome function and metabolism of sialylated conjugates that impact oligodendrocyte lineage differentiation and myelinogenesis in the central nervous system (By similarity) (PubMed:15510212, PubMed:21781115, PubMed:22778404, PubMed:23889254). Electrogenic proton-coupled nitrate symporter that transports nitrate ions across the basolateral membrane of salivary gland acinar cells, with nitrate to proton stoichiometry of 2:1. May contribute to nitrate clearance from serum by salivary glands, where it is further concentrated and secreted in the saliva (PubMed:22778404). Uses membrane potential to drive the uptake of acidic amino acids and peptides into synaptic vesicles. Responsible for synaptic vesicular storage of L-aspartate and L-glutamate in pinealocytes as well as vesicular uptake of N-acetyl-L-aspartyl-L-glutamate neuropeptide, relevant to aspartegic-associated glutamatergic neurotransmission and activation of metabotropic receptors that inhibit subsequent transmitter release (By similarity) (PubMed:21781115, PubMed:22778404, PubMed:23889254). Receptor for CM101, a polysaccharide produced by group B Streptococcus with antipathoangiogenic properties.
Alternative names
Sialin, H(+)/nitrate cotransporter, H(+)/sialic acid cotransporter, Membrane glycoprotein HP59, Solute carrier family 17 member 5, Vesicular excitatory amino acid transporter, AST, VEAT, SLC17A5
Recommended products
Rabbit Recombinant Monoclonal SLC17A5 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR27951-4
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SLC17A5 also known as sialin is a protein encoded by the SLC17A5 gene. It is a lysosomal membrane protein with an approximate molecular mass of 55 kDa. This protein facilitates the transport of sialic acid from the lysosome to the cytoplasm following degradation of sialoglycoconjugates. It gets expressed in various tissues with highest expression levels noted in the brain and kidneys.
- Biological function summary
The protein moves sialic acid a type of acidic sugar out of lysosomes important for cellular homeostasis. SLC17A5 transports not only free sialic acid but also ascorbate and other organic anions contributing to lysosomal function regulation. Though SLC17A5 functions mainly in lysosomes it can associate with membrane complexes that regulate ion transport across membranes.
- Pathways
SLC17A5 is significant within lysosomal catabolism and membrane transport pathways. In these pathways it interacts with key proteins like neuraminidase which is involved in breaking down sialoglycoconjugates. The function of sialin in moving sialic acid affects these metabolic pathways and has an impact on maintaining cellular balance.
- Associated diseases and disorders
SLC17A5 has ties to free sialic acid storage disorders notably Salla disease and infantile sialic acid storage disorder (ISSD). These are neurodegenerative disorders linked to sialic acid accumulation caused by SLC17A5 malfunction. Mutations in this gene affect its transport function leading to these diseases. Additionally SLC17A5 relates to metabolic disorders by interacting with other transmembrane proteins potentially influencing disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-SLC17A5 antibody [EPR27951-4] (ab314223), expandable thumbnail](https://content.abcam.com/products/images/slc17a5-antibody-epr27951-4-ab314223--western-blot-img421041.jpg "Western blot - Anti-SLC17A5 antibody [EPR27951-4] (ab314223)")
Western blot - Anti-SLC17A5 antibody [EPR27951-4] (ab314223)
SLC17A5 Western blot staining using rabbit Anti-SLC17A5 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: skeletal muscle (PMID: 11751519).
Samples are non-boiled as boiling may cause protein aggregation.All lanes: Western blot - Anti-SLC17A5 antibody [EPR27951-4] (ab314223) at 1/1000 dilution
Lane 1: Human hippocampus tissue lysate at 40 µg
Lane 2: Human cerebellum tissue lysate at 40 µg
Lane 3: Human skeletal muscle lysate at 40 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 54 kDa
Exposure time: 37s
![Western blot - Anti-SLC17A5 antibody [EPR27951-4] (ab314223), expandable thumbnail](https://content.abcam.com/products/images/slc17a5-antibody-epr27951-4-ab314223--western-blot-img421043.jpg "Western blot - Anti-SLC17A5 antibody [EPR27951-4] (ab314223)")
Western blot - Anti-SLC17A5 antibody [EPR27951-4] (ab314223)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.All lanes: Western blot - Anti-SLC17A5 antibody [EPR27951-4] (ab314223) at 1/1000 dilution
Lane 1: LN229 (human brain glioblastoma epithelial cell) whole cell lysate at 40 µg
Lane 2: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 40 µg
Lane 3: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 40 µg
Lane 4: bEnd.3 (mouse brain endothelioma) whole cell lysate at 40 µg
Lane 5: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 40 µg
Lane 6: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 54 kDa
Exposure time: 15s
![Western blot - Anti-SLC17A5 antibody [EPR27951-4] (ab314223), expandable thumbnail](https://content.abcam.com/products/images/slc17a5-antibody-epr27951-4-ab314223--western-blot-img421042.jpg "Western blot - Anti-SLC17A5 antibody [EPR27951-4] (ab314223)")
Western blot - Anti-SLC17A5 antibody [EPR27951-4] (ab314223)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: skeletal muscle (PMID: 11751519), liver (PMID: 22778404) spleen (PMID: 11751519).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.All lanes: Western blot - Anti-SLC17A5 antibody [EPR27951-4] (ab314223) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 40 µg
Lane 2: Mouse skeletal muscle lysate at 40 µg
Lane 3: Mouse liver lysate at 40 µg
Lane 4: Mouse spleen lysate at 40 µg
Lane 5: Rat brain tissue lysate at 40 µg
Lane 6: Rat skeletal muscle lysate at 40 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 54 kDa
Exposure time: 15s
Downloads
Product protocols
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