AB327144

Anti-SLC18A2 Antibody [CL13157]

Name: Anti-SLC18A2 Antibody [CL13157] (ab327144) | Abcam
Brand: Abcam Limited
SKU: AB327144
Price: 580 USD
Availability: InStock

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Mouse Monoclonal VMAT2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SLC18A2.

View Alternative Names

SVMT, VMAT2, SLC18A2, Synaptic vesicular amine transporter, Solute carrier family 18 member 2, Vesicular amine transporter 2, Vesicular monoamine transporter 2, VAT2

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC18A2 Antibody [CL13157] (AB327144)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC18A2 Antibody [CL13157] (AB327144)

Immunohistochemical staining of human cerebral cortex using ab327144 at 1 : 1000–1 : 2500 dilution, showing positivity in single neural fibers, and no staining in neurons as expected.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC18A2 Antibody [CL13157] (AB327144)

Immunohistochemical staining of human stomach using ab327144 at 1 : 1000–1 : 2500 dilution, showing strong cytoplasmic positivity in gastric endocrine cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC18A2 Antibody [CL13157] (AB327144)

Immunohistochemical staining of human dorsal raphe nucleus using ab327144 at 1 : 1000–1 : 2500 dilution, showing strong cytoplasmic positivity in neurons.

Supplier Data

Western blot - Anti-SLC18A2 Antibody [CL13157] (AB327144)

Western blot analysis in human cell line HEL using ab327144 at 1 µg/ml.

All lanes:

Western blot - Anti-SLC18A2 Antibody [CL13157] (ab327144)

All lanes:

Human cell line HEL

Predicted band size: 55.71 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC18A2 Antibody [CL13157] (AB327144)

Immunohistochemical staining of human skeletal muscle using ab327144 at 1 : 1000–1 : 2500 dilution, showing no positivity in myocytes as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL13157

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SLC18A2.

Q05940

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Electrogenic antiporter that exchanges one cationic monoamine with two intravesicular protons across the membrane of secretory and synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to accumulate high concentrations of monoamines inside the vesicles prior to their release via exocytosis. Transports a variety of catecholamines such as dopamine, adrenaline and noradrenaline, histamine, and indolamines such as serotonin (PubMed : 23363473, PubMed : 37914936, PubMed : 38081299, PubMed : 38517752, PubMed : 8643547). Regulates the transvesicular monoaminergic gradient that determines the quantal size. Mediates somatodendritic dopamine release in hippocampal neurons, likely as part of a regulated secretory pathway that integrates retrograde synaptic signals (By similarity). Acts as a primary transporter for striatal dopamine loading ensuring impulse-dependent release of dopamine at the synaptic cleft (By similarity). Responsible for histamine and serotonin storage and subsequent corelease from mast cell granules (PubMed : 8860238).

See full target information SLC18A2

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Product promise

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