Anti-SLC20A1 antibody [EPR27557-86]

• IP

Supplier Data

Immunoprecipitation - Anti-SLC20A1 antibody [EPR27557-86] (AB309356)

SLC20A1 was immunoprecipitated from 0.35 mg HUVEC (human umbilical vein endothelial cell) whole cell lysate with ab309356 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab309356 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HUVEC (human umbilical vein endothelial cell) whole cell lysate 10 ug
Lane 2 : ab309356 IP in HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab309356 in HUVEC whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds

All lanes:

Immunoprecipitation - Anti-SLC20A1 antibody [EPR27557-86] (ab309356) at 1/1000 dilution

All lanes:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 5s

• WB

Supplier Data

Western blot - Anti-SLC20A1 antibody [EPR27557-86] (AB309356)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 15 seconds

All lanes:

Western blot - Anti-SLC20A1 antibody [EPR27557-86] (ab309356) at 1/1000 dilution

Lane 1:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Lane 4:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 5:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 100 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-SLC20A1 antibody [EPR27557-86] (AB309356)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression tissue : skeletal muscle (PMID : 8041748). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 10 seconds

All lanes:

Western blot - Anti-SLC20A1 antibody [EPR27557-86] (ab309356) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 100 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-SLC20A1 antibody [EPR27557-86] (AB309356)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression of SLC20A1 (Target name) is upregulated in response to tunicamycin treatment (PMID : 30347511). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 6 seconds

All lanes:

Western blot - Anti-SLC20A1 antibody [EPR27557-86] (ab309356) at 1/1000 dilution

Lane 1:

Untreated HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 10 µg

Lane 2:

HEK-293 treated with /mL tunicamycin for 8 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 100 kDa

false

Exposure time: 6s