AB155259

Anti-SLC20A2/PIT2 antibody

Name: Anti-SLC20A2/PIT2 antibody (ab155259) | Abcam
Brand: Abcam Limited
SKU: AB155259

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(1 Publication)

Rabbit Polyclonal SLC20A2/PIT2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SLC20A2 aa 100-450.

View Alternative Names

GLVR2, PIT2, SLC20A2, Sodium-dependent phosphate transporter 2, Gibbon ape leukemia virus receptor 2, Phosphate transporter 2, Solute carrier family 20 member 2, GLVR-2, PiT-2, Pit2, hPit2

4 Images

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Western blot - Anti-SLC20A2/PIT2 antibody (AB155259)

7.5% SDS PAGE

All lanes:

Western blot - Anti-SLC20A2/PIT2 antibody (ab155259) at 1/500 dilution

All lanes:

293T whole cell lysate at 30 µg

Predicted band size: 70 kDa

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Supplier Data

Western blot - Anti-SLC20A2/PIT2 antibody (AB155259)

All lanes:

Western blot - Anti-SLC20A2/PIT2 antibody (ab155259) at 1/500 dilution

All lanes:

Unboiled U87-MG cell lysate at 30 µg

Predicted band size: 70 kDa

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Supplier Data

Western blot - Anti-SLC20A2/PIT2 antibody (AB155259)

All lanes:

Western blot - Anti-SLC20A2/PIT2 antibody (ab155259) at 1/500 dilution

Lane 1:

A431 whole cell extracts

Lane 2:

Unboiled A431 whole cell extracts

Predicted band size: 70 kDa

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CiteAb

Western blot - Anti-SLC20A2/PIT2 antibody (AB155259)

SLC20A2/PIT2 western blot using anti-SLC20A2/PIT2 antibody ab155259. Publication image and figure legend from Castella, B., Kopecka, J., et al., 2017, Nat Commun, PubMed 28580927.

ab155259 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab155259 please see the product overview.

ZA treatment does not affect other transporters in ZA and SIM-treated DC.Levels of the indicated plasma-membrane-associated proteins are evaluated by western blot in biotinylated extracts from untreated or 1 μM ZA and/or simvastatin (SIM) treated DC. Pancadherin is employed as a control of equal protein loading. The blots are representative of one out of the three experiments. (b) Activities of the indicated plasma-membrane-associated proteins are measured after immunopurification from the biotinylated extracts of DC treated, as shown in a. The results are expressed as nmoles hydrolysed phosphate (Pi)/min/mg proteins according to the previously prepared titration curve. The NHE1 activities and SLC4A1 activities are fluorimetrically measured, and the results are expressed as ΔpHi/min and arbitrary units, respectively. The bars represent the mean±s.e.m. of three experiments. None of the differences is statistically significant.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human SLC20A2 aa 100-450. The exact immunogen used to generate this antibody is proprietary information.

Q08357

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p>As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.</p>" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC20A2 also known as PIT2 is a sodium-dependent phosphate transporter protein with a molecular mass of approximately 80 kDa. It facilitates the uptake of inorganic phosphate into cells which is critical for various cellular functions. This protein is expressed in many tissues including the brain kidneys and lungs. In the brain SLC20A2 plays an important role in maintaining phosphate homeostasis which is necessary for neuronal activity and development.

Biological function summary

The function of this transporter protein is to maintain phosphate balance at the cellular level. It does not form any known complex with other proteins but works by itself to regulate phosphate uptake. SLC20A2 activity is essential for normal bone mineralization and energy metabolism. The phosphate transported by SLC20A2 is used in ATP production and biological signaling processes highlighting its importance in cellular metabolism.

Pathways

The role of SLC20A2 is particularly evident in the phosphate metabolism pathway. It works alongside other family proteins like SLC20A1 to regulate phosphate levels. Alteration in these pathways can impact cellular processes such as glycolysis and oxidative phosphorylation emphasizing the need for precise phosphate balance. SLC20A2 interacts with various signaling pathways that control cellular growth and differentiation showing its significance in energy and phosphate-related pathways.

Abnormal SLC20A2 function has been linked to idiopathic basal ganglia calcification a disorder characterized by calcium deposit in brain regions affecting motor control. Mutations in the SLC20A2 gene can disrupt phosphate transport leading to abnormal calcium accumulation. This disorder often involves other proteins such as XPR1 which can also be implicated in phosphate transport. Understanding the role of SLC20A2 in this context helps in developing targeted therapies for better disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sodium-phosphate symporter which preferentially transports the monovalent form of phosphate with a stoichiometry of two sodium ions per phosphate ion (PubMed : 12205090, PubMed : 15955065, PubMed : 16790504, PubMed : 17494632, PubMed : 22327515, PubMed : 28722801, PubMed : 30704756). Plays a critical role in the determination of bone quality and strength by providing phosphate for bone mineralization (By similarity). Required to maintain normal cerebrospinal fluid phosphate levels (By similarity). Mediates phosphate-induced calcification of vascular smooth muscle cells (VCMCs) and can functionally compensate for loss of SLC20A1 in VCMCs (By similarity).. (Microbial infection) Functions as a retroviral receptor and confers human cells susceptibility to infection to amphotropic murine leukemia virus (A-MuLV), 10A1 murine leukemia virus (10A1 MLV) and some feline leukemia virus subgroup B (FeLV-B) variants.

See full target information SLC20A2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 8:15663 PubMed28580927

2017

The ATP-binding cassette transporter A1 regulates phosphoantigen release and Vγ9Vδ2 T cell activation by dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Castella,Joanna Kopecka,Patrizia Sciancalepore,Giorgia Mandili,Myriam Foglietta,Nico Mitro,Donatella Caruso,Francesco Novelli,Chiara Riganti,Massimo Massaia

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SLC20A2/PIT2 antibody

Western blot - Anti-SLC20A2/PIT2 antibody (AB155259)

Western blot - Anti-SLC20A2/PIT2 antibody (AB155259)

Western blot - Anti-SLC20A2/PIT2 antibody (AB155259)

Western blot - Anti-SLC20A2/PIT2 antibody (AB155259)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

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Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Cell Lines & Lysates

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise