Rabbit Polyclonal SLC22A3/OCT3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human SLC22A3 aa 300-350.
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- Nature chemical biology 20:62-732023Cardiac contraction and relaxation are regulated by distinct subcellular cAMP pools.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing-Yu Lin,Quynh N Mai,Hao Zhang,Emily Wilson,Huan-Chieh Chien,Sook Wah Yee,Kathleen M Giacomini,Jeffrey E Olgin,Roshanak IrannejadPubMed 37474759
- Experimental gerontology 163:1118042022Short-term exposure to a clinical dose of metformin increases skeletal muscle mitochondrial HO emission and production in healthy, older adults: A randomized controlled trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlec I McKenzie,Ziad S Mahmassani,Jonathan J Petrocelli,Naomi M M P de Hart,Dennis K Fix,Patrick J Ferrara,Paul C LaStayo,Robin L Marcus,Matthew T Rondina,Scott A Summers,Jordan M Johnson,Joel D Trinity,Katsuhiko Funai,Micah J DrummondPubMed 35405248
- Irish journal of medical science 191:1115-11212021Expression and clinical significance of organic cation transporter family in glioblastoma multiforme.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingbei Lian,Shiling Xiao,Yunda Wang,Haocong Wang,Didong XiePubMed 34080124
- Fluids and barriers of the CNS 16:382019Region-specific blood-brain barrier transporter changes leads to increased sensitivity to amisulpride in Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGayathri Nair Sekhar et. al.PubMed 31842924
- Cancers 11:2019Treatment with a Combination of Metformin and 2-Deoxyglucose Upregulates Thrombospondin-1 in Microvascular Endothelial Cells: Implications in Anti-Angiogenic Cancer Therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamson Mathews Samuel et. al.PubMed 31698699
- Molecular and cellular endocrinology 499:1105912019Metformin has a direct effect on ovarian cells that is dependent on organic cation transporters.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMariana Di Pietro,Candela Velazquez,María Eugenia Matzkin,Mónica Beatriz Frungieri,Mariana Gómez Peña,Ignacio de Zúñiga,Natalia Pascuali,Griselda Irusta,María Silvia Bianchi,Fernanda Parborell,Dalhia AbramovichPubMed 31546019
- PloS one 12:e01734742017Organic cation transporter 1 (OCT1) is involved in pentamidine transport at the human and mouse blood-brain barrier (BBB).Applications:Unspecified applicationReactive species:Unspecified reactive speciesGayathri N Sekhar et. al.PubMed 28362799
- American journal of translational research 7:574-862015Reversing the reduced level of endometrial GLUT4 expression in polycystic ovary syndrome: a mechanistic study of metformin action.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Li,Peng Cui,Hong-Yuan Jiang,Yan-Rong Guo,Bano Pishdari,Min Hu,Yi Feng,Håkan Billig,Ruijin ShaoPubMed 26045896
- Journal of experimental & clinical cancer research 33:412014Direct effects of metformin in the endometrium: a hypothetical mechanism for the treatment of women with PCOS and endometrial carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuijin Shao et. al.PubMed 24887156
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SLC22A3 aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link O75751
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/25.00000 - 1/100.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Electrogenic voltage-dependent transporter that mediates the transport of a variety of organic cations such as endogenous bioactive amines, cationic drugs and xenobiotics (PubMed:10196521, PubMed:10966924, PubMed:12538837, PubMed:17460754, PubMed:20858707). Cation cellular uptake or release is driven by the electrochemical potential, i.e. membrane potential and concentration gradient (PubMed:10966924). Functions as a Na(+)- and Cl(-)-independent, bidirectional uniporter (PubMed:12538837). Implicated in monoamine neurotransmitters uptake such as dopamine, adrenaline/epinephrine, noradrenaline/norepinephrine, histamine, serotonin and tyramine, thereby supporting a role in homeostatic regulation of aminergic neurotransmission in the brain (PubMed:10196521, PubMed:16581093, PubMed:20858707). Transports dopaminergic neuromodulators cyclo(his-pro) and salsolinol with low efficiency (PubMed:17460754). May be involved in the uptake and disposition of cationic compounds by renal clearance from the blood flow (PubMed:10966924). May contribute to regulate the transport of cationic compounds in testis across the blood-testis-barrier (Probable). Mediates the transport of polyamine spermidine and putrescine (By similarity). Mediates the bidirectional transport of polyamine agmatine (PubMed:12538837). Also transports guanidine (PubMed:10966924). May also mediate intracellular transport of organic cations, thereby playing a role in amine metabolism and intracellular signaling (By similarity).
Alternative names
EMTH, OCT3, SLC22A3, Solute carrier family 22 member 3, Extraneuronal monoamine transporter, Organic cation transporter 3, EMT
Recommended products
Rabbit Polyclonal SLC22A3/OCT3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human SLC22A3 aa 300-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SLC22A3 aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link O75751
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The SLC22A3 protein also known as OCT3 or organic cation transporter 3 is involved in the transport of organic cations across cell membranes. It functions as a polyspecific transporter allowing it to transport a wide range of substrates including neurotransmitters and drugs. SLC22A3 has a molecular mass of approximately 61 kDa and is expressed in various tissues most notably in the brain heart and liver.
- Biological function summary
SLC22A3 facilitates the uptake of monoamines such as dopamine and norepinephrine into cells influencing neurotransmitter signaling and metabolism. The protein is not part of a known complex but interacts with cellular transport mechanisms. The transport capabilities impact cellular uptake processes affecting cell signaling and homeostasis.
- Pathways
SLC22A3 participates in neurotransmitter reuptake and drug metabolism pathways. Within these pathways it collaborates with transport proteins such as SLC6A4 which is responsible for serotonin uptake. SLC22A3's role in monoamine reuptake connects it to pathways regulating mood and cognitive function.
- Associated diseases and disorders
SLC22A3 links to conditions such as depression and cardiovascular diseases. Variations in its gene expression or function influence susceptibility to these conditions. In depression SLC22A3 interacts with neurotransmitter systems shared with proteins like monoamine oxidase affecting mood regulation. In cardiovascular diseases SLC22A3's expression in the heart plays a part in transporting drugs and endogenous compounds potentially impacting disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-SLC22A3/OCT3 antibody (ab183071)
All lanes: Western blot - Anti-SLC22A3/OCT3 antibody (ab183071) at 1/600 dilution
All lanes: Human liver cancer tissue lysate at 40 µg
SecondaryAll lanes: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 61 kDa
Exposure time: 1min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC22A3/OCT3 antibody (ab183071)
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling SLC22A3/OCT3 with ab183071 at a 1/40 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide. (Original magnification: ×200)
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