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AB124826

Anti-SLC22A3/OCT3 antibody [EPR6630]

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(15 Publications)

Rabbit Recombinant Monoclonal SLC22A3/OCT3 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 15 publications.

View Alternative Names

EMTH, OCT3, SLC22A3, Solute carrier family 22 member 3, Extraneuronal monoamine transporter, Organic cation transporter 3, EMT

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC22A3/OCT3 antibody [EPR6630] (AB124826)
  • IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC22A3/OCT3 antibody [EPR6630] (AB124826)

ab124826 at 1/100 dilution staining SLC22A3/OCT3 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-SLC22A3/OCT3 antibody [EPR6630] (AB124826)
  • Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-SLC22A3/OCT3 antibody [EPR6630] (AB124826)

Overlay histogram showing JEG3 cells stained with ab124826 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124826 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Western blot - Anti-SLC22A3/OCT3 antibody [EPR6630] (AB124826)
  • WB

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Western blot - Anti-SLC22A3/OCT3 antibody [EPR6630] (AB124826)

All lanes:

Western blot - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826) at 1/10000 dilution

Lane 1:

Human fetal kidney tissue lysate at 10 µg

Lane 2:

Human muscle tissue lysate at 10 µg

Lane 3:

Human placenta tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 61 kDa

Observed band size: 80 kDa

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OI-RD Scanning - Anti-SLC22A3/OCT3 antibody [EPR6630] (AB124826)
  • OI-RD Scanning

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OI-RD Scanning - Anti-SLC22A3/OCT3 antibody [EPR6630] (AB124826)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6630

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

We recommend using un-boiled sample to run WB tests. This antibody was found to have cross reactivity with SLC22A2 family member.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SLC22A3 protein also known as OCT3 or organic cation transporter 3 is involved in the transport of organic cations across cell membranes. It functions as a polyspecific transporter allowing it to transport a wide range of substrates including neurotransmitters and drugs. SLC22A3 has a molecular mass of approximately 61 kDa and is expressed in various tissues most notably in the brain heart and liver.
Biological function summary

SLC22A3 facilitates the uptake of monoamines such as dopamine and norepinephrine into cells influencing neurotransmitter signaling and metabolism. The protein is not part of a known complex but interacts with cellular transport mechanisms. The transport capabilities impact cellular uptake processes affecting cell signaling and homeostasis.

Pathways

SLC22A3 participates in neurotransmitter reuptake and drug metabolism pathways. Within these pathways it collaborates with transport proteins such as SLC6A4 which is responsible for serotonin uptake. SLC22A3's role in monoamine reuptake connects it to pathways regulating mood and cognitive function.

SLC22A3 links to conditions such as depression and cardiovascular diseases. Variations in its gene expression or function influence susceptibility to these conditions. In depression SLC22A3 interacts with neurotransmitter systems shared with proteins like monoamine oxidase affecting mood regulation. In cardiovascular diseases SLC22A3's expression in the heart plays a part in transporting drugs and endogenous compounds potentially impacting disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Electrogenic voltage-dependent transporter that mediates the transport of a variety of organic cations such as endogenous bioactive amines, cationic drugs and xenobiotics (PubMed : 10196521, PubMed : 10966924, PubMed : 12538837, PubMed : 17460754, PubMed : 20858707). Cation cellular uptake or release is driven by the electrochemical potential, i.e. membrane potential and concentration gradient (PubMed : 10966924). Functions as a Na(+)- and Cl(-)-independent, bidirectional uniporter (PubMed : 12538837). Implicated in monoamine neurotransmitters uptake such as dopamine, adrenaline/epinephrine, noradrenaline/norepinephrine, histamine, serotonin and tyramine, thereby supporting a role in homeostatic regulation of aminergic neurotransmission in the brain (PubMed : 10196521, PubMed : 16581093, PubMed : 20858707). Transports dopaminergic neuromodulators cyclo(his-pro) and salsolinol with low efficiency (PubMed : 17460754). May be involved in the uptake and disposition of cationic compounds by renal clearance from the blood flow (PubMed : 10966924). May contribute to regulate the transport of cationic compounds in testis across the blood-testis-barrier (Probable). Mediates the transport of polyamine spermidine and putrescine (By similarity). Mediates the bidirectional transport of polyamine agmatine (PubMed : 12538837). Also transports guanidine (PubMed : 10966924). May also mediate intracellular transport of organic cations, thereby playing a role in amine metabolism and intracellular signaling (By similarity).
See full target information SLC22A3
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical science 32:68 PubMed40652190

2025

Guilty by association: direct interaction with the tetraspanin CD63 suggests a role for organic cation transporter 3 in histamine release from granulocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Moritz Pernecker,Miriam Dibos,Sophie Götz,Rouvier Al-Monajjed,Vivien Barz,Christian Albiker,Rita Schröter,Ute Neugebauer,Lena Ludwig-Radtke,R Verena Taudte,Thomas Vogl,Giuliano Ciarimboli

Biomolecules 15: PubMed39858458

2025

SNP rs9364554 Modulates Androgen Receptor Binding and Drug Response in Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqian Yan,Lei Shi,Tao Ma,Liguo Wang,Haojie Huang

Endocrinology 165: PubMed37972266

2023

Increased OCT3 Expression in Adipose Tissue With Aging: Implications for Catecholamine and Lipid Turnover and Insulin Resistance in Women.

Applications

Unspecified application

Species

Unspecified reactive species

Fozia Ahmed,Milica Vranic,Susanne Hetty,Argyri Mathioudaki,Vagia Patsoukaki,Giovanni Fanni,Maria J Pereira,Jan W Eriksson

Biochemical pharmacology 217:115812 PubMed37722628

2023

Role of organic cation transporter 3 (OCT3) in the response of hepatocellular carcinoma to tyrosine kinase inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Herraez,Ruba Al-Abdulla,Meraris Soto,Oscar Briz,Dominik Bettinger,Heike Bantel,Sofia Del Carmen,Maria A Serrano,Andreas Geier,Jose J G Marin,Rocio I R Macias

Reproductive biology and endocrinology : RB&E 20:112 PubMed35927731

2022

Functional reorganization of monoamine transport systems during villous trophoblast differentiation: evidence of distinct differences between primary human trophoblasts and BeWo cells.

Applications

Unspecified application

Species

Unspecified reactive species

Veronika Vachalova,Rona Karahoda,Martina Ottaviani,Kasin Yadunandam Anandam,Cilia Abad,Christiane Albrecht,Frantisek Staud

Drug metabolism and disposition: the biological fate of chemicals 50:770-780 PubMed35307651

2022

Localization of Xenobiotic Transporters Expressed at the Human Blood-Testis Barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Raymond K Hau,Robert R Klein,Stephen H Wright,Nathan J Cherrington

Scientific reports 9:19782 PubMed31874997

2019

Protein expression of ABCC2 and SLC22A3 associates with prognosis of pancreatic adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lenka Cervenkova,Ondrej Vycital,Jan Bruha,Jachym Rosendorf,Richard Palek,Vaclav Liska,Ondrej Daum,Beatrice Mohelnikova-Duchonova,Pavel Soucek

International journal of colorectal disease 34:2151-2159 PubMed31732877

2019

Upregulated OCT3 has the potential to improve the survival of colorectal cancer patients treated with (m)FOLFOX6 adjuvant chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Gu,Dandan Dong,Enwu Long,Shiwei Tang,Suqin Feng,Tingting Li,Ling Wang,Xuehua Jiang

Oncotarget 9:743-754 PubMed29416650

2018

Organic cation transporter 3 mediates cisplatin and copper cross-resistance in hepatoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Guttmann,Gursimran Chandhok,Sara Reinartz Groba,Christoph Niemietz,Vanessa Sauer,Amanda Gomes,Giuliano Ciarimboli,Uwe Karst,Andree Zibert,Hartmut H Schmidt

Nature chemical biology 13:799-806 PubMed28553949

2017

Functional selectivity of GPCR-directed drug action through location bias.

Applications

Unspecified application

Species

Unspecified reactive species

Roshanak Irannejad,Veronica Pessino,Delphine Mika,Bo Huang,Philip B Wedegaertner,Marco Conti,Mark von Zastrow
View all publications
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