Skip to main content

Knockout Tested Rabbit Recombinant Monoclonal Slc25a1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-Slc25a1 antibody [EPR29193-92] (AB318201), expandable thumbnail
  • Western blot - Anti-Slc25a1 antibody [EPR29193-92] (AB318201), expandable thumbnail
  • Western blot - Anti-Slc25a1 antibody [EPR29193-92] (AB318201), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (AB318201), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (AB318201), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBIPICC/IF
Human
Tested
Tested
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Rat
Tested
Tested
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Citrate transporter that mediates the exchange of mitochondrial citrate for cytosolic malate (PubMed:29031613, PubMed:29238895). Also able to mediate the exchange of citrate for isocitrate, phosphoenolpyruvate, cis- but not trans-aconitate and to a lesser extend maleate and succinate (PubMed:29031613). Important for the bioenergetics of hepatic cells as it provides a carbon source for fatty acid and sterol biosyntheses, and NAD(+) for the glycolytic pathway. Required for proper neuromuscular junction formation (Probable).

Alternative names

Citrate transport protein, Solute carrier family 25 member 1, Tricarboxylate carrier protein, CTP, SLC25A1, SLC20A3

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal Slc25a1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR29193-92

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

  • Western blot - Anti-Slc25a1 antibody [EPR29193-92] (ab318201), expandable thumbnail

    Western blot - Anti-Slc25a1 antibody [EPR29193-92] (ab318201)

    In Western blot, ab318201 was shown to bind specifically to Slc25a1. Target of interest was observed at 34 kDa in wild-type HCT116 cell lysates (lane 1) with no signal observed at this size in SLC25A1 C12 knockout cell line (lane 2).

    The knockout cell line was provided, with thanks, by RESOLUTE (re-solute.eu)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Slc25a1 antibody [EPR29193-92] (ab318201) at 1/1000 dilution

    Lane 1: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: SLC25A1 C12 knockout HCT116 whole cell lysate at 20 µg

    Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 7: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Performed under reducing conditions.

    Observed band size: 34 kDa, 36 kDa

    Exposure time: 59s

  • Western blot - Anti-Slc25a1 antibody [EPR29193-92] (ab318201), expandable thumbnail

    Western blot - Anti-Slc25a1 antibody [EPR29193-92] (ab318201)

    Low expression: brain (PMID: 31959914)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Slc25a1 antibody [EPR29193-92] (ab318201) at 1/1000 dilution

    Lane 1: Human colon tissue lysate at 20 µg

    Lane 2: Human liver tissue lysate at 20 µg

    Lane 3: Human lung tissue lysate at 20 µg

    Lane 4: Mouse liver tissue lysate at 20 µg

    Lane 5: Mouse kidney tissue lysate at 20 µg

    Lane 6: Mouse brain tissue lysate at 20 µg

    Lane 7: Rat liver tissue lysate at 20 µg

    Lane 8: Rat kidney tissue lysate at 20 µg

    Lane 9: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 34 kDa, 36 kDa

    Exposure time: 48s

  • Western blot - Anti-Slc25a1 antibody [EPR29193-92] (ab318201), expandable thumbnail

    Western blot - Anti-Slc25a1 antibody [EPR29193-92] (ab318201)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-Slc25a1 antibody [EPR29193-92] (ab318201) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole lysate at 20 µg

    Lane 2: HeLa non-mitochondrial fraction at 20 µg

    Lane 3: HeLa mitochondrial fraction at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 34 kDa, 36 kDa, 15 kDa

    Exposure time: 15s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201)

    Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Slc25a1 with ab318201 at 1/1000 (0.487 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Punctated staining on human endometrium.

    The section was incubated with ab318201 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Slc25a1 with ab318201 at 1/1000 (0.487 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Punctated staining on human liver (PMID: 31959914).

    The section was incubated with ab318201 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Slc25a1 with ab318201 at 1/500 (0.974 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Punctated staining on mouse testis.

    The section was incubated with ab318201 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Slc25a1 with ab318201 at 1/500 (0.974 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Punctated staining on rat testis.

    The section was incubated with ab318201 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Slc25a1 with ab318201 at 1/1000 (0.487 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on human skeletal muscle.

    The section was incubated with ab318201 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Slc25a1 with ab318201 at 1/1000 (0.487 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: weak staining on human cerebrum.

    The section was incubated with ab318201 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201)

    Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Slc25a1 with ab318201 at 1/500 (0.974 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on mouse skeletal muscle (PMID: 31959914).

    The section was incubated with ab318201 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Slc25a1 with ab318201 at 1/500 (0.974 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: weak staining on mouse cerebrum.

    The section was incubated with ab318201 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201)

    Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Slc25a1 with ab318201 at 1/500 (0.974 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on rat skeletal muscle.

    The section was incubated with ab318201 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Slc25a1 with ab318201 at 1/500 (0.974 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: weak staining on rat cerebrum.

    The section was incubated with ab318201 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (ab318201)

    Immunohistochemical analysis of paraffin-embedded (A) Wild-type HCT116 (human colon epithelial cell) cell pellet (B) SLC25A1 C12 knockout HCT116 cell pellet tissue labeling Slc25a1 with ab318201 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Punctated staining on (A) Wild-type HCT116 cell pellet, no staining on (B) SLC25A1 C12 knockout HCT116 cell pellet.

    The section was incubated with ab318201 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    The knockout cell line was provided, with thanks, by RESOLUTE (re-solute.eu).

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com