Anti-Slc25a1 antibody [EPR29193-92]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (AB318201)

Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Slc25a1 with ab318201 at 1/1000 (0.487 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Punctated staining on human endometrium.
The section was incubated with ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (AB318201)

Immunohistochemical analysis of paraffin-embedded (A) Wild-type HCT116 (human colon epithelial cell) cell pellet (B) SLC25A1 C12 knockout HCT116 cell pellet tissue labeling Slc25a1 with ab318201 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Punctated staining on (A) Wild-type HCT116 cell pellet, no staining on (B) SLC25A1 C12 knockout HCT116 cell pellet.
The section was incubated with ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
The knockout cell line was provided, with thanks, by RESOLUTE (re-solute.eu).

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (AB318201)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Slc25a1 with ab318201 at 1/1000 (0.487 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : weak staining on human cerebrum.
The section was incubated with ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (AB318201)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Slc25a1 with ab318201 at 1/1000 (0.487 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Punctated staining on human liver (PMID : 31959914).
The section was incubated with ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (AB318201)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Slc25a1 with ab318201 at 1/500 (0.974 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Punctated staining on mouse testis.
The section was incubated with ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (AB318201)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Slc25a1 with ab318201 at 1/500 (0.974 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : weak staining on mouse cerebrum.
The section was incubated with ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (AB318201)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Slc25a1 with ab318201 at 1/500 (0.974 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Punctated staining on rat testis.
The section was incubated with ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (AB318201)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Slc25a1 with ab318201 at 1/500 (0.974 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : weak staining on rat cerebrum.
The section was incubated with ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Slc25a1 antibody [EPR29193-92] (AB318201)

Low expression : brain (PMID : 31959914)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Slc25a1 antibody [EPR29193-92] (ab318201) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 20 µg with NFDM/TBST

Lane 2:

Human liver tissue lysate at 20 µg with NFDM/TBST

Lane 3:

Human lung tissue lysate at 20 µg with NFDM/TBST

Lane 4:

Mouse liver tissue lysate at 20 µg with NFDM/TBST

Lane 5:

Mouse kidney tissue lysate at 20 µg with NFDM/TBST

Lane 6:

Mouse brain tissue lysate at 20 µg with NFDM/TBST

Lane 7:

Rat liver tissue lysate at 20 µg with NFDM/TBST

Lane 8:

Rat kidney tissue lysate at 20 µg with NFDM/TBST

Lane 9:

Rat brain tissue lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 34 kDa,36 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-Slc25a1 antibody [EPR29193-92] (AB318201)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Slc25a1 antibody [EPR29193-92] (ab318201) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole lysate at 20 µg with NFDM/TBST

Lane 2:

HeLa non-mitochondrial fraction at 20 µg with NFDM/TBST

Lane 3:

HeLa mitochondrial fraction at 20 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 34 kDa,36 kDa,15 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-Slc25a1 antibody [EPR29193-92] (AB318201)

In Western blot, ab318201 was shown to bind specifically to Slc25a1. Target of interest was observed at 34 kDa in wild-type HCT116 cell lysates (lane 1) with no signal observed at this size in SLC25A1 C12 knockout cell line (lane 2).

The knockout cell line was provided, with thanks, by RESOLUTE (re-solute.eu)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Slc25a1 antibody [EPR29193-92] (ab318201) at 1/1000 dilution

Lane 1:

HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 2:

SLC25A1 C12 knockout HCT116 whole cell lysate at 20 µg with NFDM/TBST

Lane 3:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 4:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 5:

NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 6:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with NFDM/TBST

Lane 7:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 34 kDa,36 kDa

false

Exposure time: 59s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (AB318201)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Slc25a1 with ab318201 at 1/1000 (0.487 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on human skeletal muscle.
The section was incubated with ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (AB318201)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Slc25a1 with ab318201 at 1/500 (0.974 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on rat skeletal muscle.
The section was incubated with ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc25a1 antibody [EPR29193-92] (AB318201)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Slc25a1 with ab318201 at 1/500 (0.974 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on mouse skeletal muscle (PMID : 31959914).
The section was incubated with ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins