Knockout Tested Rabbit Recombinant Monoclonal Slc25a1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
IHC-P | WB | IP | ICC/IF | |
---|---|---|---|---|
Human | Tested | Tested | Not recommended | Not recommended |
Mouse | Tested | Tested | Not recommended | Not recommended |
Rat | Tested | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Mitochondrial electroneutral antiporter that exports citrate from the mitochondria into the cytosol in exchange for malate (PubMed:29031613, PubMed:29238895). Also able to mediate the exchange of citrate for isocitrate, phosphoenolpyruvate, cis-aconitate and to a lesser extend cis-aconitate, maleate and succinate (PubMed:29031613). In the cytoplasm citrate is important in the regulation of glycolysis through a feedback mechanism and in the production of acetyl-CoA which is needed for the synthesis of fatty acids, sterols, prostaglandins, dolichol and coenzyme Q (CoQ). Required for proper neuromuscular junction formation (Probable).
SLC20A3, SLC25A1, Citrate transport protein, Mitochondrial citrate carrier, Solute carrier family 25 member 1, Tricarboxylate carrier protein, CTP, CIC
Knockout Tested Rabbit Recombinant Monoclonal Slc25a1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Yes
EPR29193-92
Affinity purification Protein A
Blue Ice
+4°C
+4°C
ab318202 is the carrier-free version of Anti-Slc25a1 antibody [EPR29193-92] ab318201.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-Slc25a1 antibody [EPR29193-92] ab318201, the same antibody clone in a different buffer formulation.
In Western blot, Anti-Slc25a1 antibody [EPR29193-92] ab318201 was shown to bind specifically to Slc25a1. Target of interest was observed at 34 kDa in wild-type HCT116 cell lysates (lane 1) with no signal observed at this size in SLC25A1 C12 knockout cell line (lane 2).
The knockout cell line was provided, with thanks, by RESOLUTE (re-solute.eu)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Slc25a1 antibody [EPR29193-92] (Anti-Slc25a1 antibody [EPR29193-92] ab318201) at 1/1000 dilution
Lane 1: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: SLC25A1 C12 knockout HCT116 whole cell lysate at 20 µg
Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 6: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 7: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Performed under reducing conditions.
Observed band size: 34 kDa, 36 kDa
Exposure time: 59s
In Western blot, Anti-Slc25a1 antibody [EPR29193-92] ab318201 was shown to bind specifically to Slc25a1. Target of interest was observed at 34 kDa in wild-type HCT116 cell lysates (lane 1) with no signal observed at this size in SLC25A1 C12 knockout cell line (lane 2).
The knockout cell line was provided, with thanks, by RESOLUTE (re-solute.eu)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
This data was developed using Anti-Slc25a1 antibody [EPR29193-92] ab318201, the same antibody clone in a different buffer formulation.
Low expression: brain (PMID: 31959914)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Slc25a1 antibody [EPR29193-92] (Anti-Slc25a1 antibody [EPR29193-92] ab318201) at 1/1000 dilution
Lane 1: Human colon tissue lysate at 20 µg
Lane 2: Human liver tissue lysate at 20 µg
Lane 3: Human lung tissue lysate at 20 µg
Lane 4: Mouse liver tissue lysate at 20 µg
Lane 5: Mouse kidney tissue lysate at 20 µg
Lane 6: Mouse brain tissue lysate at 20 µg
Lane 7: Rat liver tissue lysate at 20 µg
Lane 8: Rat kidney tissue lysate at 20 µg
Lane 9: Rat brain tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 34 kDa, 36 kDa
Exposure time: 48s
Low expression: brain (PMID: 31959914)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
This data was developed using Anti-Slc25a1 antibody [EPR29193-92] ab318201, the same antibody clone in a different buffer formulation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735) staining at 1/1000 dilution.
All lanes: Western blot - Anti-Slc25a1 antibody [EPR29193-92] (Anti-Slc25a1 antibody [EPR29193-92] ab318201) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole lysate at 20 µg
Lane 2: HeLa non-mitochondrial fraction at 20 µg
Lane 3: HeLa mitochondrial fraction at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 34 kDa, 36 kDa, 15 kDa
Exposure time: 15s
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735) staining at 1/1000 dilution.
This data was developed using Anti-Slc25a1 antibody [EPR29193-92] ab318201, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Slc25a1 with Anti-Slc25a1 antibody [EPR29193-92] ab318201 at 1/1000 (0.487 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Punctated staining on human endometrium.
The section was incubated with Anti-Slc25a1 antibody [EPR29193-92] ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Slc25a1 antibody [EPR29193-92] ab318201, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Slc25a1 with Anti-Slc25a1 antibody [EPR29193-92] ab318201 at 1/1000 (0.487 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Punctated staining on human liver (PMID: 31959914).
The section was incubated with Anti-Slc25a1 antibody [EPR29193-92] ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Slc25a1 antibody [EPR29193-92] ab318201, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Slc25a1 with Anti-Slc25a1 antibody [EPR29193-92] ab318201 at 1/500 (0.974 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Punctated staining on mouse testis.
The section was incubated with Anti-Slc25a1 antibody [EPR29193-92] ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Slc25a1 antibody [EPR29193-92] ab318201, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Slc25a1 with Anti-Slc25a1 antibody [EPR29193-92] ab318201 at 1/500 (0.974 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Punctated staining on rat testis.
The section was incubated with Anti-Slc25a1 antibody [EPR29193-92] ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Slc25a1 antibody [EPR29193-92] ab318201, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Slc25a1 with Anti-Slc25a1 antibody [EPR29193-92] ab318201 at 1/1000 (0.487 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining on human skeletal muscle.
The section was incubated with Anti-Slc25a1 antibody [EPR29193-92] ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Slc25a1 antibody [EPR29193-92] ab318201, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Slc25a1 with Anti-Slc25a1 antibody [EPR29193-92] ab318201 at 1/1000 (0.487 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: weak staining on human cerebrum.
The section was incubated with Anti-Slc25a1 antibody [EPR29193-92] ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Slc25a1 antibody [EPR29193-92] ab318201, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Slc25a1 with Anti-Slc25a1 antibody [EPR29193-92] ab318201 at 1/500 (0.974 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining on mouse skeletal muscle (PMID: 31959914).
The section was incubated with Anti-Slc25a1 antibody [EPR29193-92] ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Slc25a1 antibody [EPR29193-92] ab318201, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Slc25a1 with Anti-Slc25a1 antibody [EPR29193-92] ab318201 at 1/500 (0.974 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: weak staining on mouse cerebrum.
The section was incubated with Anti-Slc25a1 antibody [EPR29193-92] ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Slc25a1 antibody [EPR29193-92] ab318201, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Slc25a1 with Anti-Slc25a1 antibody [EPR29193-92] ab318201 at 1/500 (0.974 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining on rat skeletal muscle.
The section was incubated with Anti-Slc25a1 antibody [EPR29193-92] ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Slc25a1 antibody [EPR29193-92] ab318201, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Slc25a1 with Anti-Slc25a1 antibody [EPR29193-92] ab318201 at 1/500 (0.974 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: weak staining on rat cerebrum.
The section was incubated with Anti-Slc25a1 antibody [EPR29193-92] ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Slc25a1 antibody [EPR29193-92] ab318201, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) Wild-type HCT116 (human colon epithelial cell) cell pellet (B) SLC25A1 C12 knockout HCT116 cell pellet tissue labeling Slc25a1 with Anti-Slc25a1 antibody [EPR29193-92] ab318201 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Punctated staining on (A) Wild-type HCT116 cell pellet, no staining on (B) SLC25A1 C12 knockout HCT116 cell pellet.
The section was incubated with Anti-Slc25a1 antibody [EPR29193-92] ab318201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
The knockout cell line was provided, with thanks, by RESOLUTE (re-solute.eu).
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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