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AB234975

Anti-SLC25A12 antibody

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(2 Publications)

Rabbit Polyclonal SLC25A12 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC25A12 aa 200-350.

View Alternative Names

AGC1, ARALAR1, SLC25A12, Araceli hiperlarga, Mitochondrial aspartate glutamate carrier 1, Solute carrier family 25 member 12, Aralar, Aralar1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC25A12 antibody (AB234975)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC25A12 antibody (AB234975)

Paraffin-embedded human brain tissue stained for SLC25A12 with ab234975 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC25A12 antibody (AB234975)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC25A12 antibody (AB234975)

Paraffin-embedded human pancreatic cancer tissue stained for SLC25A12 with ab234975 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-SLC25A12 antibody (AB234975)
  • WB

Supplier Data

Western blot - Anti-SLC25A12 antibody (AB234975)

All lanes:

Western blot - Anti-SLC25A12 antibody (ab234975) at 1/500 dilution

Lane 1:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

Lane 2:

Rat heart tissue lysate

Lane 3:

Rat kidney tissue lysate

Lane 4:

Mouse kidney tissue lysate

Lane 5:

Mouse heart tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 74 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SLC25A12 aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

O75746

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC25A12 also known as the mitochondrial aspartate/glutamate carrier 1 (AGC1) functions as a transporter protein. It facilitates the exchange of aspartate and glutamate across the inner mitochondrial membrane. This protein has a molecular mass of approximately 77 kDa. SLC25A12 is mainly found in tissues with high metabolic activity including the brain heart and skeletal muscle.
Biological function summary

SLC25A12 plays an important role in energy metabolism. It is involved in transferring aspartate from the mitochondria to the cytosol and glutamate in the opposite direction which is essential for the malate-aspartate shuttle. This carrier contributes significantly to maintaining the balance of cellular metabolism by linking the citric acid cycle and amino acid synthesis to mitochondrial energy production. SLC25A12 operates as part of a larger complex of mitochondrial transport proteins which facilitate similar exchanges.

Pathways

SLC25A12 is vitally linked to the malate-aspartate shuttle and the urea cycle. In the malate-aspartate shuttle it works closely with other proteins including SLC25A13 (AGC2) to maintain NADH/NAD+ balance across the mitochondrial membrane. This balance is important for continuous ATP production during oxidative phosphorylation. The function of SLC25A12 in these pathways highlights its significance in both energy metabolism and nitrogen disposal processes interlinking with proteins involved in these metabolic pathways.

Mutations and altered expression of SLC25A12 have been associated with neurological conditions such as autism and epilepsy. Research shows a correlation between SLC25A12 dysfunction and disrupted energy metabolism in neurons which may contribute to the pathophysiology of these conditions. Furthermore interaction with proteins like SLC25A13 has been observed in some metabolic disorders emphasizing the interconnected nature of metabolic pathways and their role in disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial electrogenic aspartate/glutamate antiporter that favors efflux of aspartate and entry of glutamate and proton within the mitochondria as part of the malate-aspartate shuttle (PubMed : 11566871, PubMed : 19641205, PubMed : 24515575). Also mediates the uptake of L-cysteinesulfinate by mitochondria in exchange of L-glutamate and proton. Can also exchange L-cysteinesulfinate with aspartate in their anionic form without any proton translocation (PubMed : 11566871).
See full target information SLC25A12
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 28:e70285 PubMed39707673

2024

Melatonin Reduces Mito-Inflammation in Ischaemic Hippocampal HT22 Cells and Modulates the cGAS-STING Cytosolic DNA Sensing Pathway and FGF21 Release.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Carloni,Maria Gemma Nasoni,Anna Casabianca,Chiara Orlandi,Loredana Capobianco,Giorgia Natalia Iaconisi,Liana Cerioni,Sabrina Burattini,Serena Benedetti,Russel J Reiter,Walter Balduini,Francesca Luchetti

Human gene therapy 34:682-696 PubMed37376759

2023

An Engineered Adeno-Associated Virus Capsid Mediates Efficient Transduction of Pericytes and Smooth Muscle Cells of the Brain Vasculature.

Applications

Unspecified application

Species

Unspecified reactive species

Servio H Ramirez,Jonathan F Hale,Siobhan McCarthy,Christian L Cardenas,Kalpani N Udeni Galpayage Dona,Killian S Hanlon,Eloise Hudry,Demitri De La Cruz,Carrie Ng,Sabyasachi Das,Diane M Nguyen,Josette Nammour,Rachel E Bennett,Allison M Andrews,Patricia L Musolino,Casey A Maguire
View all publications
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