Rabbit Recombinant Monoclonal SLC25A12 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | WB | |
---|---|---|
Human | Tested | Tested |
Mouse | Expected | Tested |
Rat | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/40 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Select an associated product type
Mitochondrial electrogenic aspartate/glutamate antiporter that favors efflux of aspartate and entry of glutamate and proton within the mitochondria as part of the malate-aspartate shuttle (PubMed:11566871, PubMed:19641205, PubMed:24515575). Also mediates the uptake of L-cysteinesulfinate by mitochondria in exchange of L-glutamate and proton. Can also exchange L-cysteinesulfinate with aspartate in their anionic form without any proton translocation (PubMed:11566871).
AGC1, ARALAR1, SLC25A12, Araceli hiperlarga, Mitochondrial aspartate glutamate carrier 1, Solute carrier family 25 member 12, Aralar, Aralar1
Rabbit Recombinant Monoclonal SLC25A12 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR16294
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
SLC25A12 also known as the mitochondrial aspartate/glutamate carrier 1 (AGC1) functions as a transporter protein. It facilitates the exchange of aspartate and glutamate across the inner mitochondrial membrane. This protein has a molecular mass of approximately 77 kDa. SLC25A12 is mainly found in tissues with high metabolic activity including the brain heart and skeletal muscle.
SLC25A12 plays an important role in energy metabolism. It is involved in transferring aspartate from the mitochondria to the cytosol and glutamate in the opposite direction which is essential for the malate-aspartate shuttle. This carrier contributes significantly to maintaining the balance of cellular metabolism by linking the citric acid cycle and amino acid synthesis to mitochondrial energy production. SLC25A12 operates as part of a larger complex of mitochondrial transport proteins which facilitate similar exchanges.
SLC25A12 is vitally linked to the malate-aspartate shuttle and the urea cycle. In the malate-aspartate shuttle it works closely with other proteins including SLC25A13 (AGC2) to maintain NADH/NAD+ balance across the mitochondrial membrane. This balance is important for continuous ATP production during oxidative phosphorylation. The function of SLC25A12 in these pathways highlights its significance in both energy metabolism and nitrogen disposal processes interlinking with proteins involved in these metabolic pathways.
Mutations and altered expression of SLC25A12 have been associated with neurological conditions such as autism and epilepsy. Research shows a correlation between SLC25A12 dysfunction and disrupted energy metabolism in neurons which may contribute to the pathophysiology of these conditions. Furthermore interaction with proteins like SLC25A13 has been observed in some metabolic disorders emphasizing the interconnected nature of metabolic pathways and their role in disease.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: SLC25A12 knockout HAP1 cell lysate (20 μg)
Lane 3: Human skeletal muscle lysate (20 μg)
Lane 4: A431 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab200201 observed at 72 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab200201 was shown to recognize SLC25A12 when SLC25A12 knockout samples were used, along with additional cross-reactive bands. Wild-type and SLC25A12 knockout samples were subjected to SDS-PAGE. ab200201 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 andGoat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-SLC25A12 antibody [EPR16294] (ab200201)
Predicted band size: 74 kDa
Observed band size: 72 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-SLC25A12 antibody [EPR16294] (ab200201) at 1/5000 dilution
Lane 1: Human fetal heart lysate at 20 µg
Lane 2: Human skeletal muscle lysate at 20 µg
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 74 kDa
Observed band size: 75 kDa
Exposure time: 10s
SLC25A12 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200201 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab200201 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Jurkat whole cell lysate 10 μg (Input).
Lane 2: ab200201 IP in Jurkat whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab200201 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 second.
All lanes: Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] (ab200201)
Predicted band size: 74 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-SLC25A12 antibody [EPR16294] (ab200201) at 1/1000 dilution
Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 2: A431 (Human epidermoid carcinoma) whole cell lysate at 10 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 74 kDa
Observed band size: 75 kDa
Exposure time: 3min
SLC25A12 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200201 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab200201 at 1/1000 dilution (Panel A) or ab192244 at 1/1000 dilution (Panel B).
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Jurkat whole cell lysate 10 μg (Input).
Lane 2: ab200201 IP in Jurkat whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab200201 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time (Panel A and Panel B): 30 second.
All lanes: Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] (ab200201)
Predicted band size: 74 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-SLC25A12 antibody [EPR16294] (ab200201) at 1/1000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: Mouse heart lysate at 10 µg
Lane 3: Mouse kidney lysate at 10 µg
Lane 4: Rat brain lysate at 10 µg
Lane 5: Rat heart lysate at 10 µg
Lane 6: Rat kidney lysate at 10 µg
Lane 7: C6 (Rat glial tumor cells) whole cell lysate at 10 µg
Lane 8: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 9: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg
Lane 10: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 74 kDa
Observed band size: 75 kDa
Exposure time: 15s
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