Skip to main content

Rabbit Recombinant Monoclonal SLC25A12 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (AB246329), expandable thumbnail
  • Western blot - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (AB246329), expandable thumbnail
  • Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (AB246329), expandable thumbnail
  • Western blot - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (AB246329), expandable thumbnail
  • Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (AB246329), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/40
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Mitochondrial electrogenic aspartate/glutamate antiporter that favors efflux of aspartate and entry of glutamate and proton within the mitochondria as part of the malate-aspartate shuttle (PubMed:11566871, PubMed:19641205, PubMed:24515575). Also mediates the uptake of L-cysteinesulfinate by mitochondria in exchange of L-glutamate and proton. Can also exchange L-cysteinesulfinate with aspartate in their anionic form without any proton translocation (PubMed:11566871).

Alternative names

AGC1, ARALAR1, SLC25A12, Araceli hiperlarga, Mitochondrial aspartate glutamate carrier 1, Solute carrier family 25 member 12, Aralar, Aralar1

Recommended products

Rabbit Recombinant Monoclonal SLC25A12 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR16294
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab246329 is the carrier-free version of Anti-SLC25A12 antibody [EPR16294] ab200201.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SLC25A12 also known as the mitochondrial aspartate/glutamate carrier 1 (AGC1) functions as a transporter protein. It facilitates the exchange of aspartate and glutamate across the inner mitochondrial membrane. This protein has a molecular mass of approximately 77 kDa. SLC25A12 is mainly found in tissues with high metabolic activity including the brain heart and skeletal muscle.

Biological function summary

SLC25A12 plays an important role in energy metabolism. It is involved in transferring aspartate from the mitochondria to the cytosol and glutamate in the opposite direction which is essential for the malate-aspartate shuttle. This carrier contributes significantly to maintaining the balance of cellular metabolism by linking the citric acid cycle and amino acid synthesis to mitochondrial energy production. SLC25A12 operates as part of a larger complex of mitochondrial transport proteins which facilitate similar exchanges.

Pathways

SLC25A12 is vitally linked to the malate-aspartate shuttle and the urea cycle. In the malate-aspartate shuttle it works closely with other proteins including SLC25A13 (AGC2) to maintain NADH/NAD+ balance across the mitochondrial membrane. This balance is important for continuous ATP production during oxidative phosphorylation. The function of SLC25A12 in these pathways highlights its significance in both energy metabolism and nitrogen disposal processes interlinking with proteins involved in these metabolic pathways.

Associated diseases and disorders

Mutations and altered expression of SLC25A12 have been associated with neurological conditions such as autism and epilepsy. Research shows a correlation between SLC25A12 dysfunction and disrupted energy metabolism in neurons which may contribute to the pathophysiology of these conditions. Furthermore interaction with proteins like SLC25A13 has been observed in some metabolic disorders emphasizing the interconnected nature of metabolic pathways and their role in disease.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329), expandable thumbnail

    Western blot - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)

    This data was developed using Anti-SLC25A12 antibody [EPR16294] ab200201, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: SLC25A12 knockout HAP1 cell lysate (20 μg)
    Lane 3: Human skeletal muscle lysate (20 μg)
    Lane 4: A431 cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - Anti-SLC25A12 antibody [EPR16294] ab200201 observed at 72 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    Anti-SLC25A12 antibody [EPR16294] ab200201 was shown to recognize SLC25A12 when SLC25A12 knockout samples were used, along with additional cross-reactive bands. Wild-type and SLC25A12 knockout samples were subjected to SDS-PAGE. Anti-SLC25A12 antibody [EPR16294] ab200201 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 andGoat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)

    Predicted band size: 74 kDa

    Observed band size: 72 kDa

  • Western blot - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329), expandable thumbnail

    Western blot - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)

    This data was developed using Anti-SLC25A12 antibody [EPR16294] ab200201, the same antibody clone in a different buffer formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329) at 1/5000 dilution

    Lane 1: Human fetal heart lysate at 20 µg

    Lane 2: Human skeletal muscle lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 74 kDa

    Observed band size: 75 kDa

    Exposure time: 10s

  • Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329), expandable thumbnail

    Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)

    SLC25A12 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with Anti-SLC25A12 antibody [EPR16294] ab200201 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using Anti-SLC25A12 antibody [EPR16294] ab200201 at 1/1000 dilution.

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Jurkat whole cell lysate 10 μg (Input).

    Lane 2: Anti-SLC25A12 antibody [EPR16294] ab200201 IP in Jurkat whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SLC25A12 antibody [EPR16294] ab200201 in Jurkat whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SLC25A12 antibody [EPR16294] ab200201).

    All lanes: Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] (Anti-SLC25A12 antibody [EPR16294] ab200201)

    Predicted band size: 74 kDa

  • Western blot - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329), expandable thumbnail

    Western blot - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)

    This data was developed using Anti-SLC25A12 antibody [EPR16294] ab200201, the same antibody clone in a different buffer formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329) at 1/1000 dilution

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

    Lane 2: A431 (Human epidermoid carcinoma) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 74 kDa

    Observed band size: 75 kDa

    Exposure time: 3min

  • Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329), expandable thumbnail

    Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)

    SLC25A12 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with Anti-SLC25A12 antibody [EPR16294] ab200201 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using Anti-SLC25A12 antibody [EPR16294] ab200201 at 1/1000 dilution (Panel A) or ab192244 at 1/1000 dilution (Panel B).

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Jurkat whole cell lysate 10 μg (Input).

    Lane 2: Anti-SLC25A12 antibody [EPR16294] ab200201 IP in Jurkat whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SLC25A12 antibody [EPR16294] ab200201 in Jurkat whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time (Panel A and Panel B): 30 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SLC25A12 antibody [EPR16294] ab200201).

    All lanes: Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] (Anti-SLC25A12 antibody [EPR16294] ab200201)

    Predicted band size: 74 kDa

  • Western blot - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329), expandable thumbnail

    Western blot - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)

    This data was developed using Anti-SLC25A12 antibody [EPR16294] ab200201, the same antibody clone in a different buffer formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Mouse heart lysate at 10 µg

    Lane 3: Mouse kidney lysate at 10 µg

    Lane 4: Rat brain lysate at 10 µg

    Lane 5: Rat heart lysate at 10 µg

    Lane 6: Rat kidney lysate at 10 µg

    Lane 7: C6 (Rat glial tumor cells) whole cell lysate at 10 µg

    Lane 8: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 9: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

    Lane 10: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 74 kDa

    Observed band size: 75 kDa

    Exposure time: 15s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com