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Rabbit Recombinant Monoclonal SLC25A13/Citrin antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (AB167166), expandable thumbnail
  • Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (AB167166), expandable thumbnail
  • Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (AB167166), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Mitochondrial electrogenic aspartate/glutamate antiporter that favors efflux of aspartate and entry of glutamate and proton within the mitochondria as part of the malate-aspartate shuttle (PubMed:11566871). Also mediates the uptake of L-cysteinesulfinate by mitochondria in exchange of L-glutamate and proton. Can also exchange L-cysteinesulfinate with aspartate in their anionic form without any proton translocation (PubMed:11566871).

Alternative names

Calcium-binding mitochondrial carrier protein Aralar2, Citrin, Mitochondrial aspartate glutamate carrier 2, Solute carrier family 25 member 13, ARALAR-related gene 2, ARALAR2, SLC25A13

Recommended products

Rabbit Recombinant Monoclonal SLC25A13/Citrin antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR9969(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The SLC25A13 protein also known as Citrin is a member of the solute carrier family 25 involved in transporting solutes across the mitochondrial membrane. Citrin functions as a calcium-dependent aspartate/glutamate carrier. It is around 67 kDa in mass. Citrin is primarily expressed in the liver heart and pancreas where it facilitates the exchange of metabolites necessary for cellular metabolism. The protein is localized to the inner mitochondrial membrane where it takes part in important cellular processes.

Biological function summary

Citrin plays an important role in the urea cycle gluconeogenesis and lipid metabolism by facilitating the exchange of cytosolic glutamate for mitochondrial aspartate. It operates independently not as part of a larger protein complex. By ensuring the appropriate balance of amino acids and metabolites Citrin contributes to maintaining metabolic homeostasis. The protein's activity supports the liver's function and influences energy production efficiency especially under anabolic conditions.

Pathways

Citrin is integral to the malate-aspartate shuttle and the urea cycle. The malate-aspartate shuttle is involved in transferring reducing equivalents across the mitochondrial membrane which is essential for efficient ATP production. Citrin interacts closely with mitochondrial enzymes like carbamoyl phosphate synthetase I in the urea cycle emphasizing its link to ammonia detoxification. Citrin's transport activity indirectly supports the function of other mitochondrial carriers including SLC25A12 and SLC25A11 by maintaining the requisite balance of substrates required for their processes.

Associated diseases and disorders

Citrin deficiency is associated with two genetic conditions: Citrullinemia Type II and Neonatal Intrahepatic Cholestasis caused by Citrin Deficiency (NICCD). Both disorders arise from mutations in the SLC25A13 gene and disrupt normal urea cycle function leading to the accumulation of toxic substances like ammonia. Citrin's interaction with proteins involved in amino acid metabolism including SLC25A15 (ornithine transporter) highlights its role in maintaining nitrogen balance. Understanding Citrin's activity can aid in diagnosing and treating metabolic disorders linked to urea cycle dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166), expandable thumbnail

    Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166)

    Lanes 1- 2: Merged signal (red and green). Green - ab167166 observed at 70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab167166 was shown to react with SLC25A13 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human SLC25A13 (Citrin) knockout HeLa cell line ab265668 (knockout cell lysate Human SLC25A13 (Citrin) knockout HeLa cell lysate ab258192) was used. Wild-type HeLa and SLC25A13 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab167166 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: SLC25A13 knockout HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 74 kDa

    Observed band size: 70 kDa

  • Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166), expandable thumbnail

    Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166)

    Lanes 1 - 3: Merged signal (red and green). Green - ab167166 observed at 70 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
    ab167166 was shown to recognize SLC25A13 when SLC25A13 knockout samples were used, along with additional cross-reactive bands. Wild-type and SLC25A13 knockout samples were subjected to SDS-PAGE. ab167166 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166) at 1/500 dilution

    Lane 1: Wild-type HAP1 cell lysate at 20 µg

    Lane 2: SLC25A13 knockout HAP1 cell lysate at 20 µg

    Lane 3: SH-5YSY cell lysate at 20 µg

    Predicted band size: 74 kDa

  • Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166), expandable thumbnail

    Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166)

    All lanes: Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166) at 1/1000 dilution

    Lane 1: Human fetal liver lysates at 10 µg

    Lane 2: HepG2 cell lysates at 10 µg

    Lane 3: SH-SY5Y cell lysates at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 74 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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