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AB167166

Anti-SLC25A13/Citrin antibody [EPR9969(B)]

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(2 Publications)

Rabbit Recombinant Monoclonal SLC25A13/Citrin antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Calcium-binding mitochondrial carrier protein Aralar2, Citrin, Mitochondrial aspartate glutamate carrier 2, Solute carrier family 25 member 13, ARALAR-related gene 2, ARALAR2, SLC25A13

3 Images
Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (AB167166)
  • WB

Lab

Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (AB167166)

Lanes 1- 2 : Merged signal (red and green). Green - ab167166 observed at 70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab167166 was shown to react with SLC25A13 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265668 (knockout cell lysate ab258192) was used. Wild-type HeLa and SLC25A13 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab167166 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

SLC25A13 knockout HeLa cell lysate at 20 µg

Predicted band size: 74 kDa

Observed band size: 70 kDa

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Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (AB167166)
  • WB

Lab

Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (AB167166)

Lanes 1 - 3 : Merged signal (red and green). Green - ab167166 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab167166 was shown to recognize SLC25A13 when SLC25A13 knockout samples were used, along with additional cross-reactive bands. Wild-type and SLC25A13 knockout samples were subjected to SDS-PAGE. ab167166 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166) at 1/500 dilution

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

SLC25A13 knockout HAP1 cell lysate at 20 µg

Lane 3:

SH-5YSY cell lysate at 20 µg

Predicted band size: 74 kDa

false

Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (AB167166)
  • WB

Unknown

Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (AB167166)

All lanes:

Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166) at 1/1000 dilution

Lane 1:

Human fetal liver lysates at 10 µg

Lane 2:

HepG2 cell lysates at 10 µg

Lane 3:

SH-SY5Y cell lysates at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 74 kDa

false

  • Carrier free

    Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9969(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SLC25A13 protein also known as Citrin is a member of the solute carrier family 25 involved in transporting solutes across the mitochondrial membrane. Citrin functions as a calcium-dependent aspartate/glutamate carrier. It is around 67 kDa in mass. Citrin is primarily expressed in the liver heart and pancreas where it facilitates the exchange of metabolites necessary for cellular metabolism. The protein is localized to the inner mitochondrial membrane where it takes part in important cellular processes.
Biological function summary

Citrin plays an important role in the urea cycle gluconeogenesis and lipid metabolism by facilitating the exchange of cytosolic glutamate for mitochondrial aspartate. It operates independently not as part of a larger protein complex. By ensuring the appropriate balance of amino acids and metabolites Citrin contributes to maintaining metabolic homeostasis. The protein's activity supports the liver's function and influences energy production efficiency especially under anabolic conditions.

Pathways

Citrin is integral to the malate-aspartate shuttle and the urea cycle. The malate-aspartate shuttle is involved in transferring reducing equivalents across the mitochondrial membrane which is essential for efficient ATP production. Citrin interacts closely with mitochondrial enzymes like carbamoyl phosphate synthetase I in the urea cycle emphasizing its link to ammonia detoxification. Citrin's transport activity indirectly supports the function of other mitochondrial carriers including SLC25A12 and SLC25A11 by maintaining the requisite balance of substrates required for their processes.

Citrin deficiency is associated with two genetic conditions: Citrullinemia Type II and Neonatal Intrahepatic Cholestasis caused by Citrin Deficiency (NICCD). Both disorders arise from mutations in the SLC25A13 gene and disrupt normal urea cycle function leading to the accumulation of toxic substances like ammonia. Citrin's interaction with proteins involved in amino acid metabolism including SLC25A15 (ornithine transporter) highlights its role in maintaining nitrogen balance. Understanding Citrin's activity can aid in diagnosing and treating metabolic disorders linked to urea cycle dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial electrogenic aspartate/glutamate antiporter that favors efflux of aspartate and entry of glutamate and proton within the mitochondria as part of the malate-aspartate shuttle (PubMed : 11566871). Also mediates the uptake of L-cysteinesulfinate by mitochondria in exchange of L-glutamate and proton. Can also exchange L-cysteinesulfinate with aspartate in their anionic form without any proton translocation (PubMed : 11566871).
See full target information SLC25A13
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Human molecular genetics 32:1922-1931 PubMed36881658

2023

Nicotinamide riboside rescues dysregulated glycolysis and fatty acid β-oxidation in a human hepatic cell model of citrin deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Winifred W Yau,Gao Bin Chen,Jin Zhou,Joel Celio Francisco,Nivetha Kanakaram Thimmukonda,Shang Li,Brijesh Kumar Singh,Paul Michael Yen

Frontiers in pharmacology 13:931282 PubMed36071835

2022

Identification and validation of key biomarkers for the early diagnosis of diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yu,Ting Wang,Feng Wu,Yiding Zhang,Jin Shang,Zhanzheng Zhao
View all publications
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