Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free

• WB

Lab

Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free (AB249408)

This data was developed using the same antibody clone in a different buffer formulation (ab167166).

Lanes 1 - 3 : Merged signal (red and green). Green - ab167166 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab167166 was shown to recognize SLC25A13 when SLC25A13 knockout samples were used, along with additional cross-reactive bands. Wild-type and SLC25A13 knockout samples were subjected to SDS-PAGE. ab167166 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free (ab249408) at 1/500 dilution

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

SLC25A13 knockout HAP1 cell lysate at 20 µg

Lane 3:

SH-5YSY cell lysate at 20 µg

Predicted band size: 74 kDa

false

• WB

Lab

Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free (AB249408)

This data was developed using the same antibody clone in a different buffer formulation (ab167166).

Lanes 1- 2 : Merged signal (red and green). Green - ab167166 observed at 70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab167166 was shown to react with SLC25A13 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265668 (knockout cell lysate ab258192) was used. Wild-type HeLa and SLC25A13 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab167166 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (<a href='/en-us/products/primary-antibodies/slc25a13-citrin-antibody-epr9969b-ab167166'>ab167166</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

SLC25A13 knockout HeLa cell lysate at 20 µg

Predicted band size: 74 kDa

Observed band size: 70 kDa

false