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AB230545

Anti-SLC25A6 antibody

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(2 Publications)

Rabbit Polyclonal SLC25A6 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SLC25A6 aa 100-200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

AAC3, ANT3, CDABP0051, SLC25A6, ADP/ATP translocase 3, Adenine nucleotide translocator 3, Solute carrier family 25 member 6, ANT 2, ANT 3

4 Images
Flow Cytometry (Intracellular) - Anti-SLC25A6 antibody (AB230545)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SLC25A6 antibody (AB230545)

Intracellular Flow Cytometry analysis of A549 (Human lung carcinoma cell line) cells, labeling SLC25A6 with ab230545 at a 1/10 dilution (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SLC25A6 antibody (AB230545)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SLC25A6 antibody (AB230545)

A549 (Human lung carcinoma cell line) cells stained for SLC25A6 using ab230545 at a dilution of 1/10 in ICC/IF, followed by AlexaFluor® 488-conjugated goat anti-rabbit lgG (green).

DAPI was used to stain the cell nucleus (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC25A6 antibody (AB230545)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC25A6 antibody (AB230545)

Formalin-fixed, paraffin-embedded human adipose tissue stained for SLC25A6 with ab230545 at 1/10 dilution in immunohistochemical analysis.

Western blot - Anti-SLC25A6 antibody (AB230545)
  • WB

Supplier Data

Western blot - Anti-SLC25A6 antibody (AB230545)

All lanes:

Western blot - Anti-SLC25A6 antibody (ab230545) at 1/1000 dilution

All lanes:

A549 (human lung carcinoma cell line) cell lysates at 35 µg

Predicted band size: 33 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SLC25A6 aa 100-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P12236

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/10 - 1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ADP : ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell (By similarity). Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state) : operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane (By similarity). In addition to its ADP : ATP antiporter activity, also involved in mitochondrial uncoupling and mitochondrial permeability transition pore (mPTP) activity (PubMed : 15033708). Plays a role in mitochondrial uncoupling by acting as a proton transporter : proton transport uncouples the proton flows via the electron transport chain and ATP synthase to reduce the efficiency of ATP production and cause mitochondrial thermogenesis (By similarity). Proton transporter activity is inhibited by ADP : ATP antiporter activity, suggesting that SLC25A6/ANT3 acts as a master regulator of mitochondrial energy output by maintaining a delicate balance between ATP production (ADP : ATP antiporter activity) and thermogenesis (proton transporter activity) (By similarity). Proton transporter activity requires free fatty acids as cofactor, but does not transport it (By similarity). Also plays a key role in mPTP opening, a non-specific pore that enables free passage of the mitochondrial membranes to solutes of up to 1.5 kDa, and which contributes to cell death (PubMed : 15033708). It is however unclear if SLC25A6/ANT3 constitutes a pore-forming component of mPTP or regulates it (By similarity).
See full target information SLC25A6
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 39:1631-1640 PubMed38018881

2023

Methyltransferase-like 3-mediated m6A modification of miR-1908-5p contributes to nasopharyngeal carcinoma progression by targeting homeodomain-only protein homeobox.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanhong Zhou,Wei Li

Journal of cell science 134: PubMed33526710

2021

Adenine nucleotide translocase regulates airway epithelial metabolism, surface hydration and ciliary function.

Applications

Unspecified application

Species

Unspecified reactive species

Corrine R Kliment,Jennifer M K Nguyen,Mary Jane Kaltreider,YaWen Lu,Steven M Claypool,Josiah E Radder,Frank C Sciurba,Yingze Zhang,Alyssa D Gregory,Pablo A Iglesias,Venkataramana K Sidhaye,Douglas N Robinson
View all publications
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