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Rabbit Recombinant Monoclonal SLC26A2/DTD antibody. Carrier free. Suitable for WB, IP and reacts with Human samples.

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Images

Western blot - Anti-SLC26A2/DTD antibody [EPR27119-17] - BSA and Azide free (AB308626), expandable thumbnail
  • Western blot - Anti-SLC26A2/DTD antibody [EPR27119-17] - BSA and Azide free (AB308626), expandable thumbnail
  • Western blot - Anti-SLC26A2/DTD antibody [EPR27119-17] - BSA and Azide free (AB308626), expandable thumbnail
  • Western blot - Anti-SLC26A2/DTD antibody [EPR27119-17] - BSA and Azide free (AB308626), expandable thumbnail
  • Immunoprecipitation - Anti-SLC26A2/DTD antibody [EPR27119-17] - BSA and Azide free (AB308626), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PICC/IF
Human
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Sulfate transporter which mediates sulfate uptake into chondrocytes in order to maintain adequate sulfation of proteoglycans which is needed for cartilage development (PubMed:11448940, PubMed:15294877, PubMed:20219950, PubMed:7923357). Mediates electroneutral anion exchange of sulfate ions for oxalate ions and of sulfate and oxalate ions for chloride ions (PubMed:20219950). Mediates exchange of sulfate and oxalate ions for hydroxyl ions and of chloride ions for bromide, iodide and nitrate ions (By similarity). The coupling of sulfate transport to both hydroxyl and chloride ions likely serves to ensure transport at both acidic pH when most sulfate uptake is mediated by sulfate-hydroxide exchange and alkaline pH when most sulfate uptake is mediated by sulfate-chloride exchange (By similarity). Essential for chondrocyte proliferation, differentiation and cell size expansion (By similarity).

Alternative names

DTD, DTDST, SLC26A2, Sulfate transporter, Diastrophic dysplasia protein, Solute carrier family 26 member 2

Recommended products

Rabbit Recombinant Monoclonal SLC26A2/DTD antibody. Carrier free. Suitable for WB, IP and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27119-17
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-SLC26A2/DTD antibody [EPR27119-17] - BSA and Azide free (ab308626), expandable thumbnail

    Western blot - Anti-SLC26A2/DTD antibody [EPR27119-17] - BSA and Azide free (ab308626)

    This data was developed using Anti-SLC26A2/DTD antibody [EPR27119-17] ab308625, the same antibody clone in a different buffer formulation.

    Western blot: Anti-SLC26A2 antibody [EPR27119-17] (Anti-SLC26A2/DTD antibody [EPR27119-17] ab308625) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-SLC26A2/DTD antibody [EPR27119-17] ab308625 was shown to bind specifically to SLC26A2. A band was observed at 82 kDa in wild-type A549 cell lysates with no signal observed at this size in SLC26A2 knockout cell line. To generate this image, wild-type and SLC26A2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution. For optimal results, we recommend using fluorescent western blot (TBS-based) blocking solution and not boiling the samples.

    All lanes: Western blot - Anti-SLC26A2/DTD antibody [EPR27119-17] (Anti-SLC26A2/DTD antibody [EPR27119-17] ab308625) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: SLC26A2 knockout A549 cell lysate at 20 µg

    Lane 3: HeLa UNBOILED cell lysate at 20 µg

    Lane 4: HEK-293 UNBOILED cell lysate at 20 µg

    Secondary

    Lanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

    Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 82 kDa

  • Western blot - Anti-SLC26A2/DTD antibody [EPR27119-17] - BSA and Azide free (ab308626), expandable thumbnail

    Western blot - Anti-SLC26A2/DTD antibody [EPR27119-17] - BSA and Azide free (ab308626)

    This data was developed using 308625, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    SLC26A2 is a glycoprotein of approximately 100 kDa and detected as a 80 kDa band after treated with Protein Deglycosylation MIX II.

    Samples are non-boiled as boiling may cause protein aggregation.

    Exposure time: 37 seconds

    All lanes: Western blot - Anti-SLC26A2/DTD antibody [EPR27119-17] (Anti-SLC26A2/DTD antibody [EPR27119-17] ab308625) at 1/1000 dilution

    Lane 1: Untreated human colon tissue lysate at 20 µg

    Lane 2: Human colon tissue lysate treated with Protein Deglycosylation MIXII at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 81 kDa

    Observed band size: 100 kDa, 80 kDa

    Exposure time: 37s

  • Western blot - Anti-SLC26A2/DTD antibody [EPR27119-17] - BSA and Azide free (ab308626), expandable thumbnail

    Western blot - Anti-SLC26A2/DTD antibody [EPR27119-17] - BSA and Azide free (ab308626)

    This data was developed using 308625, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: Liver tissue (PMID:24302720, PMID:11457925) Stomach tissue.

    Samples are non-boiled as boiling may cause protein aggregation.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    Exposure time: 26 seconds

    All lanes: Western blot - Anti-SLC26A2/DTD antibody [EPR27119-17] (Anti-SLC26A2/DTD antibody [EPR27119-17] ab308625) at 1/1000 dilution

    Lane 1: Human colon tissue lysate at 20 µg

    Lane 3: Human liver tissue lysate at 20 µg

    Lane 3: Human stomach tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 81 kDa

    Observed band size: 100 kDa

    Exposure time: 26s

  • Western blot - Anti-SLC26A2/DTD antibody [EPR27119-17] - BSA and Azide free (ab308626), expandable thumbnail

    Western blot - Anti-SLC26A2/DTD antibody [EPR27119-17] - BSA and Azide free (ab308626)

    This data was developed using 308625, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: 293T.

    Samples are non-boiled as boiling may cause protein aggregation.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    Exposure time: 10 seconds

    All lanes: Western blot - Anti-SLC26A2/DTD antibody [EPR27119-17] (Anti-SLC26A2/DTD antibody [EPR27119-17] ab308625) at 1/1000 dilution

    Lane 1: A375 (human malignant melanoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 81 kDa

    Observed band size: 80, 100 kDa

    Exposure time: 10s

  • Immunoprecipitation - Anti-SLC26A2/DTD antibody [EPR27119-17] - BSA and Azide free (ab308626), expandable thumbnail

    Immunoprecipitation - Anti-SLC26A2/DTD antibody [EPR27119-17] - BSA and Azide free (ab308626)

    This data was developed using Anti-SLC26A2/DTD antibody [EPR27119-17] ab308625, the same antibody clone in a different buffer formulation.SLC26A2/DTD was immunoprecipitated from 0.35 mg Human colon tissue lysate with Anti-SLC26A2/DTD antibody [EPR27119-17] ab308625 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-SLC26A2/DTD antibody [EPR27119-17] ab308625 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Human colon tissue lysate

    Lane 2: abAB308625 IP in Human colon tissue lysate

    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SLC26A2/DTD antibody [EPR27119-17] ab308625 in human colon tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 180 seconds

    Bands around 300 kDa may be SLC26A2 oligomers.

    All lanes: Immunoprecipitation - Anti-SLC26A2/DTD antibody [EPR27119-17] (Anti-SLC26A2/DTD antibody [EPR27119-17] ab308625) at 1/30 dilution

    All lanes: Human colon tissue lysate at 10 µg

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 100 kDa, 300 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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