Anti-SLC27A4 / FATP4 antibody [EPR17319-26] - BSA and Azide free

• WB

Lab

Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] - BSA and Azide free (AB251312)

This data was developed using the same antibody clone in a different buffer formulation (ab200353).

Lanes 1-4 : Merged signal (red and green). Green - ab200353 observed at 72 kDa. Red - loading control ab8245 observed at 36 kDa.

ab200353 Anti-SLC27A4 / FATP4 antibody [EPR17319-26] was shown to specifically react with SLC27A4 / FATP4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266114 (knockout cell lysate ab257677) was used. Wild-type and SLC27A4 / FATP4 knockout samples were subjected to SDS-PAGE. ab200353 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (<a href='/en-us/products/primary-antibodies/slc27a4-fatp4-antibody-epr17319-26-ab200353'>ab200353</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

SLC27A4 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human SLC27A4 (FATP4) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-slc27a4-fatp4-knockout-hek-293t-cell-line-ab266114'>ab266114</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

HEK-293 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 72 kDa

Observed band size: 72 kDa

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