Anti-SLC32A1/VGAT antibody [EPR26258-9]

11 product images

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  Immunocytochemistry/ Immunofluorescence - Anti-SLC32A1/VGAT antibody [EPR26258-9] (ab308062)

  SLC32A1/VGAT Immunocytochemistry/ Immunofluorescence staining of mouse primary neural/glia cell using rabbit Anti-SLC32A1/VGAT antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cells labelling SLC32A1/VGAT with ab308062 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).
  Confocal image showing positive staining in mouse primary neural/glia cell.
  Confocal scanning Z step was set as 0.3 µM followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution, follwed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
  
  -ve control 1: ab308062 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at a 1/1000 dilution.
  -ve control 2: Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at a 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-SLC32A1/VGAT antibody [EPR26258-9] (ab308062)

  SLC32A1/VGAT Immunocytochemistry/ Immunofluorescence staining of rat primary neural/glia cell using rabbit Anti-SLC32A1/VGAT antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cells labelling SLC32A1/VGAT with ab308062 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).
  Confocal image showing positive staining in rat primary neural/glia cell.
  Confocal scanning Z step was set as 0.3 µM followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution, follwed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
  
  -ve control 1: ab308062 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at a 1/1000 dilution.
  -ve control 2: Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at a 1/1000 dilution.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC32A1/VGAT antibody [EPR26258-9] (ab308062)

  Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling SLC32A1/VGAT with ab308062 at 1/500 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat hippocampus (PMID: 9822734). The section was incubated with ab308062 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC32A1/VGAT antibody [EPR26258-9] (ab308062)

  Immunohistochemical analysis of paraffin-embedded human hippocampus tissue labeling SLC32A1/VGAT with ab308062 at 1/500 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human hippocampus. The section was incubated with ab308062 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC32A1/VGAT antibody [EPR26258-9] (ab308062)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling SLC32A1/VGAT with ab308062 at 1/500 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on rat liver. The section was incubated with ab308062 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC32A1/VGAT antibody [EPR26258-9] (ab308062)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SLC32A1/VGAT with ab308062 at 1/500 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on mouse liver. The section was incubated with ab308062 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC32A1/VGAT antibody [EPR26258-9] (ab308062)

  Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling SLC32A1/VGAT with ab308062 at 1/500 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse hippocampus (PMID: 29859117). The section was incubated with ab308062 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [EPR26258-9] (ab308062)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh) tissue labeling SLC32A1/VGAT with ab308062 at 1/50 dilution (10.3 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).
  
  Confocal image showing positive staining on rat hippocampus.
  
  The nuclear counterstain was DAPI (Blue). The section was incubated with ab308062 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). 
  
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).

• 

  Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [EPR26258-9] (ab308062)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling SLC32A1/VGAT with ab308062 at 1/50 dilution (10.3 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).
  
  Negative control: confocal image showing no staining on rat liver (PMID: 9349821). 
  
  The nuclear counterstain was DAPI (Blue). The section was incubated with ab308062 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). 
  
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).

• 

  Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [EPR26258-9] (ab308062)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh) tissue labeling SLC32A1/VGAT with ab308062 at 1/50 dilution (10.3 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).
  
  Confocal image showing positive staining on mouse hippocampus.
  
  The nuclear counterstain was DAPI (Blue). The section was incubated with ab308062 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). 
  
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).

• 

  Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [EPR26258-9] (ab308062)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling SLC32A1/VGAT with ab308062 at 1/50 dilution (10.3 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).
  
  Negative control: confocal image showing no staining on mouse liver (PMID: 9349821). 
  
  The nuclear counterstain was DAPI (Blue). The section was incubated with ab308062 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). 
  
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).