Anti-SLC32A1/VGAT antibody [L118/80]

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [L118/80] (AB307448)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling SLC32A1/VGAT with ab307448 at 1/50 dilution followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Secondary antibody control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbe. Confocal image showing positive staining on rat cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307448 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC

Supplier Data

Immunohistochemistry - Anti-SLC32A1/VGAT antibody [L118/80] (AB307448)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SLC32A1/VGAT with ab307448 at 1/10000 dilution (0.0946 ug/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on mouse liver is observed. The section was incubated with ab307448 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-SLC32A1/VGAT antibody [L118/80] (AB307448)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling SLC32A1/VGAT with ab307448 at 1/10000 dilution (0.0946 ug/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection. Positive staining on rat cerebrum is observed. The section was incubated with ab307448 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-SLC32A1/VGAT antibody [L118/80] (AB307448)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling SLC32A1/VGAT with ab307448 at 1/10000 dilution (0.0946 ug/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum is observed. The section was incubated with ab307448 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [L118/80] (AB307448)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling SLC32A1/VGAT with ab307448 at 1/50 dilution followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Secondary antibody control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbe. Confocal image showing positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307448 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [L118/80] (AB307448)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling SLC32A1/VGAT with ab307448 at 1/50 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Secondary antibody control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbe. Negative control : confocal image showing no staining on rat liver (PMID : 9822734). The nuclear counterstain was DAPI (Blue). The section was incubated with ab307448 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [L118/80] (AB307448)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling SLC32A1/VGAT with ab307448 at 1/50 dilution followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Secondary antibody control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbe. Negative control : confocal image showing no staining on mouse liver (PMID : 9822734). The nuclear counterstain was DAPI (Blue). The section was incubated with ab307448 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SLC32A1/VGAT antibody [L118/80] (AB307448)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labeling SLC32A1/VGAT with ab307448 at 1/1000 dilution (0.946 ug/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 ug/ml). Confocal image showing positive staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (1 ug/ml), followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (2 ug/ml) (Red). Nuclear counterstain was DAPI (Blue). -ve control 1 : ab307448 at 1/1000 dilution followed by ab150088 at 1/1000 dilution. -ve control 2 : ab183830 at 1/1000 dilution followed by ab150117 at 1/1000 dilution.

• WB

Lab

Western blot - Anti-SLC32A1/VGAT antibody [L118/80] (AB307448)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregates.

Negative control : liver tissue (PMID : 9822734), spleen, lung tissue (PMID : 9349821).

All lanes:

Western blot - Anti-SLC32A1/VGAT antibody [L118/80] (ab307448) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse lung tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat lung tissue lysate at 20 µg

Lane 6:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Observed band size: 57 kDa

false

Exposure time: 80s