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Mouse Recombinant Monoclonal SLC32A1/VGAT antibody. Suitable for ICC/IF, IHC-P, WB, IHC-Fr and reacts with Mouse, Rat samples.

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Images

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-PWBIHC-Fr
Human
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Tested
Rat
Not recommended
Tested
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/10000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/10000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Rat

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

-

Species

Rat

Dilution info

1/50

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Target data

Function

Involved in the uptake of GABA and glycine into the synaptic vesicles.

Alternative names

Recommended products

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Mouse Recombinant Monoclonal SLC32A1/VGAT antibody. Suitable for ICC/IF, IHC-P, WB, IHC-Fr and reacts with Mouse, Rat samples.

Alternative names

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

L118/80

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-SLC32A1/VGAT antibody [L118/80] (ab307448), expandable thumbnail

    Western blot - Anti-SLC32A1/VGAT antibody [L118/80] (ab307448)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: liver tissue (PMID: 9822734), spleen, lung tissue (PMID: 9349821).

    The identity of the lower MW band at approximately 37 kDa is unknown.

    Samples are non-boiled as boiling may cause protein aggregates.

    This blot was developed using a high sensitivity ECL substrate.

    All lanes: Western blot - Anti-SLC32A1/VGAT antibody [L118/80] (AB307448) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate 20 μg

    Lane 2: Mouse liver tissue lysate 20 μg

    Lane 3: Mouse spleen tissue lysate 20 μg

    Lane 4: Mouse lu tissue lysate 20 μg

    Lane 5: Rat hippocampus tissue lysate 20 μg

    Lane 6: Rat liver tissue lysate 20 μg

    Developed using the ECL technique.

    Observed band size: 57 kDa

    Exposure time: 3min

  • Immunohistochemistry - Anti-SLC32A1/VGAT antibody [L118/80] (ab307448), expandable thumbnail

    Immunohistochemistry - Anti-SLC32A1/VGAT antibody [L118/80] (ab307448)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling SLC32A1/VGAT with ab307448 at 1/10000 dilution (0.0946 ug/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection.
    Positive staining on rat cerebrum is observed.
    The section was incubated with ab307448 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

  • Immunohistochemistry - Anti-SLC32A1/VGAT antibody [L118/80] (ab307448), expandable thumbnail

    Immunohistochemistry - Anti-SLC32A1/VGAT antibody [L118/80] (ab307448)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SLC32A1/VGAT with ab307448 at 1/10000 dilution (0.0946 ug/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Negative control: No staining on mouse liver is observed.
    The section was incubated with ab307448 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

  • Immunohistochemistry - Anti-SLC32A1/VGAT antibody [L118/80] (ab307448), expandable thumbnail

    Immunohistochemistry - Anti-SLC32A1/VGAT antibody [L118/80] (ab307448)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling SLC32A1/VGAT with ab307448 at 1/10000 dilution (0.0946 ug/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Positive staining on mouse cerebrum is observed.
    The section was incubated with ab307448 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-SLC32A1/VGAT antibody [L118/80] (ab307448), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SLC32A1/VGAT antibody [L118/80] (ab307448)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labeling SLC32A1/VGAT with ab307448 at 1/1000 dilution (0.946 ug/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 ug/ml).
    Confocal image showing positive staining in mouse primary neuron.
    Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (1 ug/ml), followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (2 ug/ml) (Red). Nuclear counterstain was DAPI (Blue).
    -ve control 1: ab307448 at 1/1000 dilution followed by ab150088 at 1/1000 dilution.
    -ve control 2: ab183830 at 1/1000 dilution followed by ab150117 at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [L118/80] (ab307448), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [L118/80] (ab307448)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling SLC32A1/VGAT with ab307448 at 1/50 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Secondary antibody control: Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbe.

    Negative control: confocal image showing no staining on rat liver (PMID: 9822734). The nuclear counterstain was DAPI (Blue). The section was incubated with ab307448 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [L118/80] (ab307448), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [L118/80] (ab307448)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling SLC32A1/VGAT with ab307448 at 1/50 dilution followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Secondary antibody control: Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbe.

    Confocal image showing positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307448 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [L118/80] (ab307448), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [L118/80] (ab307448)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling SLC32A1/VGAT with ab307448 at 1/50 dilution followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Secondary antibody control: Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbe.

    Negative control: confocal image showing no staining on mouse liver (PMID: 9822734). The nuclear counterstain was DAPI (Blue). The section was incubated with ab307448 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [L118/80] (ab307448), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [L118/80] (ab307448)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling SLC32A1/VGAT with ab307448 at 1/50 dilution followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Secondary antibody control: Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbe.

    Confocal image showing positive staining on rat cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307448 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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