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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Mouse Recombinant Monoclonal SLC32A1/VGAT antibody. Suitable for ICC/IF, IHC-P, WB, IHC-Fr and reacts with Mouse, Rat samples.
IgG2a
Mouse
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
ICC/IF | IHC-P | WB | IHC-Fr | |
---|---|---|---|---|
Human | Not recommended | Not recommended | Not recommended | Not recommended |
Mouse | Tested | Tested | Tested | Tested |
Rat | Not recommended | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/10000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/10000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
Species Rat | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Involved in the uptake of GABA and glycine into the synaptic vesicles.
Vesicular inhibitory amino acid transporter, GABA and glycine transporter, Solute carrier family 32 member 1, Vesicular GABA transporter, mVGAT, mVIAAT, Vgat, Slc32a1, Viaat
Mouse Recombinant Monoclonal SLC32A1/VGAT antibody. Suitable for ICC/IF, IHC-P, WB, IHC-Fr and reacts with Mouse, Rat samples.
Vesicular inhibitory amino acid transporter, GABA and glycine transporter, Solute carrier family 32 member 1, Vesicular GABA transporter, mVGAT, mVIAAT, Vgat, Slc32a1, Viaat
IgG2a
Mouse
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
L118/80
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: liver tissue (PMID: 9822734), spleen, lung tissue (PMID: 9349821).
The identity of the lower MW band at approximately 37 kDa is unknown.
Samples are non-boiled as boiling may cause protein aggregates.
This blot was developed using a high sensitivity ECL substrate.
All lanes: Western blot - Anti-SLC32A1/VGAT antibody [L118/80] (AB307448) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate 20 μg
Lane 2: Mouse liver tissue lysate 20 μg
Lane 3: Mouse spleen tissue lysate 20 μg
Lane 4: Mouse lu tissue lysate 20 μg
Lane 5: Rat hippocampus tissue lysate 20 μg
Lane 6: Rat liver tissue lysate 20 μg
Developed using the ECL technique.
Observed band size: 57 kDa
Exposure time: 3min
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling SLC32A1/VGAT with ab307448 at 1/10000 dilution (0.0946 ug/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection.
Positive staining on rat cerebrum is observed.
The section was incubated with ab307448 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SLC32A1/VGAT with ab307448 at 1/10000 dilution (0.0946 ug/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining on mouse liver is observed.
The section was incubated with ab307448 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling SLC32A1/VGAT with ab307448 at 1/10000 dilution (0.0946 ug/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum is observed.
The section was incubated with ab307448 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labeling SLC32A1/VGAT with ab307448 at 1/1000 dilution (0.946 ug/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 ug/ml).
Confocal image showing positive staining in mouse primary neuron.
Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (1 ug/ml), followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (2 ug/ml) (Red). Nuclear counterstain was DAPI (Blue).
-ve control 1: ab307448 at 1/1000 dilution followed by ab150088 at 1/1000 dilution.
-ve control 2: ab183830 at 1/1000 dilution followed by ab150117 at 1/1000 dilution.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling SLC32A1/VGAT with ab307448 at 1/50 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Secondary antibody control: Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbe.
Negative control: confocal image showing no staining on rat liver (PMID: 9822734). The nuclear counterstain was DAPI (Blue). The section was incubated with ab307448 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling SLC32A1/VGAT with ab307448 at 1/50 dilution followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Secondary antibody control: Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbe.
Confocal image showing positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307448 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling SLC32A1/VGAT with ab307448 at 1/50 dilution followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Secondary antibody control: Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbe.
Negative control: confocal image showing no staining on mouse liver (PMID: 9822734). The nuclear counterstain was DAPI (Blue). The section was incubated with ab307448 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling SLC32A1/VGAT with ab307448 at 1/50 dilution followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Secondary antibody control: Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbe.
Confocal image showing positive staining on rat cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307448 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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