Anti-SLC32A1/VGAT antibody [L118/80] - BSA and Azide free (ab307449)

Mouse Recombinant Monoclonal SLC32A1/VGAT antibody. Carrier free. Suitable for IHC-Fr, ICC/IF, IHC-P, WB and reacts with Mouse, Rat samples.

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Images

Western blot - Anti-SLC32A1/VGAT antibody [L118/80] - BSA and Azide free (AB307449), expandable thumbnail

Key facts

Isotype: IgG2a
Host species: Mouse
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-Fr	ICC/IF	IHC-P	WB
Human	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Tested
Rat	Tested	Not recommended	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Target data

See full target information Slc32a1

Function

Antiporter that exchanges vesicular protons for cytosolic 4-aminobutanoate or to a lesser extend glycine, thus allowing their secretion from nerve terminals (PubMed:16701208, PubMed:26912364, PubMed:27601664, PubMed:9395291). The transport is equally dependent on the chemical and electrical components of the proton gradient (PubMed:27601664, PubMed:9395291). May also transport beta-alanine (By similarity). Acidification of GABAergic synaptic vesicles is a prerequisite for 4-aminobutanoate uptake (PubMed:27601664).

Alternative names

Vgat, Viaat, Slc32a1, Vesicular inhibitory amino acid transporter, Solute carrier family 32 member 1, Vesicular GABA and glycine transporter, Vesicular GABA transporter, mVGAT, mVIAAT

Recommended products

Mouse Recombinant Monoclonal SLC32A1/VGAT antibody. Carrier free. Suitable for IHC-Fr, ICC/IF, IHC-P, WB and reacts with Mouse, Rat samples.

Key facts

Isotype: IgG2a
Host species: Mouse
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: L118/80
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Western blot - Anti-SLC32A1/VGAT antibody [L118/80] - BSA and Azide free (ab307449)
SLC32A1/VGAT Western blot staining using mouse Anti-SLC32A1/VGAT antibody
This data was developed using Anti-SLC32A1/VGAT antibody [L118/80] ab307448, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Samples are non-boiled as boiling may cause protein aggregates.
Negative control: liver tissue (PMID: 9822734), spleen, lung tissue (PMID: 9349821).
All lanes: Western blot - Anti-SLC32A1/VGAT antibody [L118/80] (Anti-SLC32A1/VGAT antibody [L118/80] ab307448) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse lung tissue lysate at 20 µg
Lane 3: Mouse liver tissue lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
Lane 5: Rat lung tissue lysate at 20 µg
Lane 6: Rat liver tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 57 kDa
Exposure time: 80s
Immunohistochemistry - Anti-SLC32A1/VGAT antibody [L118/80] - BSA and Azide free (ab307449)
This data was developed using Anti-SLC32A1/VGAT antibody [L118/80] ab307448, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling SLC32A1/VGAT with Anti-SLC32A1/VGAT antibody [L118/80] ab307448 at 1/10000 dilution (0.0946 ug/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat cerebrum is observed.
The section was incubated with Anti-SLC32A1/VGAT antibody [L118/80] ab307448 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry - Anti-SLC32A1/VGAT antibody [L118/80] - BSA and Azide free (ab307449)
This data was developed using Anti-SLC32A1/VGAT antibody [L118/80] ab307448, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SLC32A1/VGAT with Anti-SLC32A1/VGAT antibody [L118/80] ab307448 at 1/10000 dilution (0.0946 ug/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining on mouse liver is observed.
The section was incubated with Anti-SLC32A1/VGAT antibody [L118/80] ab307448 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry - Anti-SLC32A1/VGAT antibody [L118/80] - BSA and Azide free (ab307449)
This data was developed using Anti-SLC32A1/VGAT antibody [L118/80] ab307448, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling SLC32A1/VGAT with Anti-SLC32A1/VGAT antibody [L118/80] ab307448 at 1/10000 dilution (0.0946 ug/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum is observed.
The section was incubated with Anti-SLC32A1/VGAT antibody [L118/80] ab307448 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunocytochemistry/ Immunofluorescence - Anti-SLC32A1/VGAT antibody [L118/80] - BSA and Azide free (ab307449)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labeling SLC32A1/VGAT with Anti-SLC32A1/VGAT antibody [L118/80] ab307448 at 1/1000 dilution (0.946 ug/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 ug/ml).
Confocal image showing positive staining in mouse primary neuron.
Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. Anti-MAP2 antibody [EPR19691] - Neuronal Marker ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (1 ug/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (2 ug/ml) (Red). Nuclear counterstain was DAPI (Blue).
-ve control 1: Anti-SLC32A1/VGAT antibody [L118/80] ab307448 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 at 1/1000 dilution.
-ve control 2: Anti-MAP2 antibody [EPR19691] - Neuronal Marker ab183830 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [L118/80] - BSA and Azide free (ab307449)
This data was developed using Anti-SLC32A1/VGAT antibody [L118/80] ab307448, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling SLC32A1/VGAT with Anti-SLC32A1/VGAT antibody [L118/80] ab307448 at 1/50 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Secondary antibody control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbe.

Negative control: confocal image showing no staining on rat liver (PMID: 9822734). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-SLC32A1/VGAT antibody [L118/80] ab307448 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [L118/80] - BSA and Azide free (ab307449)
This data was developed using Anti-SLC32A1/VGAT antibody [L118/80] ab307448, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling SLC32A1/VGAT with Anti-SLC32A1/VGAT antibody [L118/80] ab307448 at 1/50 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Secondary antibody control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbe.

Confocal image showing positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-SLC32A1/VGAT antibody [L118/80] ab307448 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [L118/80] - BSA and Azide free (ab307449)
This data was developed using Anti-SLC32A1/VGAT antibody [L118/80] ab307448, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling SLC32A1/VGAT with Anti-SLC32A1/VGAT antibody [L118/80] ab307448 at 1/50 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Secondary antibody control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbe.

Negative control: confocal image showing no staining on mouse liver (PMID: 9822734). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-SLC32A1/VGAT antibody [L118/80] ab307448 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunohistochemistry (Frozen sections) - Anti-SLC32A1/VGAT antibody [L118/80] - BSA and Azide free (ab307449)
This data was developed using Anti-SLC32A1/VGAT antibody [L118/80] ab307448, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling SLC32A1/VGAT with Anti-SLC32A1/VGAT antibody [L118/80] ab307448 at 1/50 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Secondary antibody control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbe.

Confocal image showing positive staining on rat cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-SLC32A1/VGAT antibody [L118/80] ab307448 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).