Anti-SLC34A2 antibody [SP322] - BSA and Azide free

11 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - BSA and Azide free (ab238793)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human endometrial carcinoma tissue sections labeling SLC34A2 with Anti-SLC34A2 antibody [SP322] - N-terminal ab228474 at 1/100 dilution (2.37 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the luminal surface of human endometrial carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
  The section was incubated with Anti-SLC34A2 antibody [SP322] - N-terminal ab228474 for 10 mins at room temperature. This image was generated using Anti-SLC34A2 antibody [SP322] - N-terminal ab228474, the same clone, but with a different buffer formulation.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - BSA and Azide free (ab238793)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling SLC34A2 with Anti-SLC34A2 antibody [SP322] - N-terminal ab228474 at 1/100 dilution (2.37 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human lung carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
  The section was incubated with Anti-SLC34A2 antibody [SP322] - N-terminal ab228474 for 10 mins at room temperature. This image was generated using Anti-SLC34A2 antibody [SP322] - N-terminal ab228474, the same clone, but with a different buffer formulation.

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  Immunocytochemistry/ Immunofluorescence - Anti-SLC34A2 antibody [SP322] - BSA and Azide free (ab238793)

  Immunocytochemistry/ Immunofluorescence analysis of NIH/OVCAR-3 (human ovary adenocarcinoma epithelial cell) cells labeling SLC34A2 with purified Anti-SLC34A2 antibody [SP322] - N-terminal ab228474 at 1/100 (2.4 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SLC34A2 antibody [SP322] - N-terminal ab228474).

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  Flow Cytometry (Intracellular) - Anti-SLC34A2 antibody [SP322] - BSA and Azide free (ab238793)

  Intracellular Flow Cytometry analysis of OvCAR-3 (human ovary adenocarcinoma) labeling SLC34A2 with purified Anti-SLC34A2 antibody [SP322] - N-terminal ab228474 at 1/20 dilution (11.85μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black). Unlableled control - Unlabelled cells (blue).
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SLC34A2 antibody [SP322] - N-terminal ab228474).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - BSA and Azide free (ab238793)

  Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for SLC34A2 using Anti-SLC34A2 antibody [SP322] - N-terminal ab228474 at 1/100 dilution in immunohistochemical analysis.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-SLC34A2 antibody [SP322] - N-terminal ab228474).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - BSA and Azide free (ab238793)

  Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for SLC34A2 using Anti-SLC34A2 antibody [SP322] - N-terminal ab228474 at 1/100 dilution in immunohistochemical analysis.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SLC34A2 antibody [SP322] - N-terminal ab228474).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - BSA and Azide free (ab238793)

  Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for SLC34A2 using Anti-SLC34A2 antibody [SP322] - N-terminal ab228474 at 1/100 dilution in immunohistochemical analysis.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SLC34A2 antibody [SP322] - N-terminal ab228474).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - BSA and Azide free (ab238793)

  Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for SLC34A2 using Anti-SLC34A2 antibody [SP322] - N-terminal ab228474 at 1/100 dilution in immunohistochemical analysis.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SLC34A2 antibody [SP322] - N-terminal ab228474).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - BSA and Azide free (ab238793)

  Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for SLC34A2 using Anti-SLC34A2 antibody [SP322] - N-terminal ab228474 at 1/100 dilution in immunohistochemical analysis.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SLC34A2 antibody [SP322] - N-terminal ab228474).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - BSA and Azide free (ab238793)

  Formalin-fixed, paraffin-embedded human uterus tissue stained for SLC34A2 using Anti-SLC34A2 antibody [SP322] - N-terminal ab228474 at 1/100 dilution in immunohistochemical analysis.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SLC34A2 antibody [SP322] - N-terminal ab228474).

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  Multiplex immunohistochemistry - Anti-SLC34A2 antibody [SP322] - BSA and Azide free (ab238793)

  Fluorescence multiplex immunohistochemical analysis of the human endometrium (Formalin/PFA-fixed paraffin-embedded sections).
  
  Panel A: merged staining of anti-E Cadherin (Anti-E Cadherin antibody [EP700Y] - BSA and Azide free ab256580, red; Opal™690), anti-SLC34A2 (ab238793, green; Opal™520) and anti-CD10 (Anti-CD10 antibody [EPR22865-73] ab255609, cyan; Opal™570) on human endometrium. Panel B: anti-CD10 stained on stromal cells. Panel C: anti-E Cadherin stained on glandular cells. Panel D: anti-SLC34A2 stained on apical membrane of glandular cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
  
  The section was incubated in three rounds of staining: in the order of Anti-E Cadherin antibody [EP700Y] - BSA and Azide free ab256580 at 1/3000 dilution  (0.324 μg/ml) for 30mins, ab238793 at 1/1000 dilution (2.26 μg/ml) for 10mins and Anti-CD10 antibody [EPR22865-73] ab255609 at 1/1000 dilution (0.615 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.