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Rabbit Recombinant Monoclonal SLC34A2 antibody. N-terminal. Suitable for mIHC, Flow Cyt (Intra), IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCFlow Cyt (Intra)IHC-PICC/IF
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/20

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary antibody incubation for 10 minutes at room temperature.

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Product

Target data

Function

May be involved in actively transporting phosphate into cells via Na(+) cotransport. It may be the main phosphate transport protein in the intestinal brush border membrane. May have a role in the synthesis of surfactant in lungs' alveoli.

Alternative names

Sodium-dependent phosphate transport protein 2B, Sodium-phosphate transport protein 2B, Na(+)-dependent phosphate cotransporter 2B, NaPi3b, Sodium/phosphate cotransporter 2B, Solute carrier family 34 member 2, Na(+)/Pi cotransporter 2B, NaPi-2b, SLC34A2

Recommended products

Rabbit Recombinant Monoclonal SLC34A2 antibody. N-terminal. Suitable for mIHC, Flow Cyt (Intra), IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication.

Alternative names

Sodium-dependent phosphate transport protein 2B, Sodium-phosphate transport protein 2B, Na(+)-dependent phosphate cotransporter 2B, NaPi3b, Sodium/phosphate cotransporter 2B, Solute carrier family 34 member 2, Na(+)/Pi cotransporter 2B, NaPi-2b, SLC34A2

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

SP322

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human endometrial carcinoma tissue sections labeling SLC34A2 with ab228474 at 1/100 dilution (2.37 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the luminal surface of human endometrial carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab228474 for 10 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling SLC34A2 with ab228474 at 1/100 dilution (2.37 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human lung carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab228474 for 10 mins at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474)

    Immunocytochemistry/ Immunofluorescence analysis of NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell) cells labeling SLC34A2 with purified ab228474 at 1/100 (2.4 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474)

    Flow cytometry analysis of OvCAR-3 (human ovary adenocarcinoma) labeling SLC34A2 with purified ab228474 at 1/20 dilution (11.85 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black). Unlableled control -Unlabelled cells (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474)

    Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474)

    Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474)

    Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474)

    Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474)

    Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474)

    Formalin-fixed, paraffin-embedded human uterus tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

  • Multiplex immunohistochemistry - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474), expandable thumbnail

    Multiplex immunohistochemistry - Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474)

    Fluorescence multiplex immunohistochemical analysis of the human endometrium (Formalin/PFA-fixed paraffin-embedded sections).

    Panel A: merged staining of anti-E Cadherin (Anti-E Cadherin antibody [EP700Y] - BSA and Azide free ab256580, red; Opal™690), anti-SLC34A2 (Anti-SLC34A2 antibody [SP322] - BSA and Azide free ab238793, green; Opal™520) and anti-CD10 (Anti-CD10 antibody [EPR22865-73] ab255609, cyan; Opal™570) on human endometrium. Panel B: anti-CD10 stained on stromal cells. Panel C: anti-E Cadherin stained on glandular cells. Panel D: anti-SLC34A2 stained on apical membrane of glandular cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

    The section was incubated in three rounds of staining: in the order of Anti-E Cadherin antibody [EP700Y] - BSA and Azide free ab256580 at 1/3000 dilution  (0.324 μg/ml) for 30mins, Anti-SLC34A2 antibody [SP322] - BSA and Azide free ab238793 at 1/1000 dilution (2.26 μg/ml) for 10mins and Anti-CD10 antibody [EPR22865-73] ab255609 at 1/1000 dilution (0.615 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.

    This image was generated using Anti-SLC34A2 antibody [SP322] - BSA and Azide free ab238793, the same clone, but with a different buffer formulation.

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