AB228474

Anti-SLC34A2 antibody [SP322] - N-terminal

Name: Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474) | Abcam
Brand: Abcam Limited
SKU: AB228474
Price: 220 USD
Availability: InStock
Rating: 5 (2 reviews)

BOND RX™ Validated
20ul selling size
RabMAb
Advanced Validation
Recombinant
What is this?

(2 Reviews)

(2 Publications)

Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474) is a rabbit monoclonal antibody detecting SLC34A2 in Flow Cytometry (Intra), IHC-P, ICC/IF, mIHC. Suitable for Human.

- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Sodium-dependent phosphate transport protein 2B, Sodium-phosphate transport protein 2B, Na(+)-dependent phosphate cotransporter 2B, NaPi3b, Sodium/phosphate cotransporter 2B, Solute carrier family 34 member 2, Na(+)/Pi cotransporter 2B, NaPi-2b, SLC34A2

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human endometrial carcinoma tissue sections labeling SLC34A2 with ab228474 at 1/100 dilution (2.37 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the luminal surface of human endometrial carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab228474 for 10 mins at room temperature.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunocytochemistry/ Immunofluorescence analysis of NIH : OVCAR-3 (human ovary adenocarcinoma epithelial cell) cells labeling SLC34A2 with purified ab228474 at 1/100 (2.4 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Formalin-fixed, paraffin-embedded human uterus tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling SLC34A2 with ab228474 at 1/100 dilution (2.37 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human lung carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab228474 for 10 mins at room temperature.

Flow Cytometry (Intracellular) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Flow cytometry analysis of OvCAR-3 (human ovary adenocarcinoma) labeling SLC34A2 with purified ab228474 at 1/20 dilution (11.85 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (Black). Unlableled control -Unlabelled cells (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

Multiplex immunohistochemistry - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Fluorescence multiplex immunohistochemical analysis of the human endometrium (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-E Cadherin (ab256580, red; Opal™690), anti-SLC34A2 (ab238793, green; Opal™520) and anti-CD10 (ab255609, cyan; Opal™570) on human endometrium. Panel B : anti-CD10 stained on stromal cells. Panel C : anti-E Cadherin stained on glandular cells. Panel D : anti-SLC34A2 stained on apical membrane of glandular cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab256580 at 1/3000 dilution (0.324 μg/ml) for 30mins, ab238793 at 1/1000 dilution (2.26 μg/ml) for 10mins and ab255609 at 1/1000 dilution (0.615 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. This image was generated using ab238793, the same clone, but with a different buffer formulation.

Carrier free

Anti-SLC34A2 antibody [SP322] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-SLC34A2 - N-terminal antibody [SP322]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-SLC34A2 - N-terminal antibody [SP322]
578 PE

PE Anti-SLC34A2 antibody [SP322] - N-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP322

Isotype

IgG

Carrier free

Reacts with

Human

Applications

mIHC, Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.Primary antibody incubation for 10 minutes at room temperature.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), Multiplex IHC (mIHC) in Human samples.

Collaboration
Anti-SLC34A2 [SP322] - N-terminal (ab228474) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [SP322] also available for your convenience: ab228474, Carrier free - ab238793, Alexa Fluor® 488 - ab315178, Alexa Fluor® 647 - ab315179, PE - ab322453

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in actively transporting phosphate into cells via Na(+) cotransport.

See full target information SLC34A2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Analytical cellular pathology (Amsterdam) 2021:3198555 PubMed34336552

2021

Evaluation of the Prognostic Value of Solute Carrier Family 34 Member 2 "SLC34A2" in Papillary Thyroid Carcinoma: An Immunohistochemical Study.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Adel Hakim,Rasha Mohamed Abd El Atti,Reham Mohamed Faheim,Hoda Hassan Abou Gabal

Cancer immunology, immunotherapy : CII 68:1621-1633 PubMed31549213

2019

The BRCA2 mutation status shapes the immune phenotype of prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Maximilian Jenzer,Peter Keß,Cathleen Nientiedt,Volker Endris,Maximilian Kippenberger,Jonas Leichsenring,Fabian Stögbauer,Josh Haimes,Skyler Mishkin,Brian Kudlow,Adam Kaczorowski,Stefanie Zschäbitz,Anna-Lena Volckmar,Holger Sültmann,Dirk Jäger,Anette Duensing,Peter Schirmacher,Markus Hohenfellner,Carsten Grüllich,Albrecht Stenzinger,Stefan Duensing

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLC34A2 antibody [SP322] - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunocytochemistry/ Immunofluorescence - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Flow Cytometry (Intracellular) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Multiplex immunohistochemistry - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise