Rabbit Polyclonal SLC36A1 antibody. N-terminal. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC36A1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- iScience 26:1060452023NRF3 activates mTORC1 arginine-dependently for cancer cell viability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuuhei Hirose,Tsuyoshi Waku,Misato Tani,Haruka Masuda,Keiko Endo,Sanae Ashitani,Iori Aketa,Hina Kitano,Sota Nakada,Ayaka Wada,Atsushi Hatanaka,Tsuyoshi Osawa,Tomoyoshi Soga,Akira KobayashiPubMed 36818298
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SLC36A1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z2H8
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10.00000 - 1/50.00000 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target data
Function
Electrogenic proton/amino acid symporter with selectivity for small apolar L-amino acids, their D-enantiomers and selected amino acid derivatives such as 4-aminobutanoate/GABA (PubMed:12527723, PubMed:12809675, PubMed:19549785). May be involved in the efflux from the lysosomal compartment of neutral amino acids resulting from proteolysis (By similarity). May play a role in specifying sites for exocytosis in neurons (By similarity).
Alternative names
PAT1, SLC36A1, Proton-coupled amino acid transporter 1, Proton/amino acid transporter 1, hPAT1, Solute carrier family 36 member 1
Recommended products
Rabbit Polyclonal SLC36A1 antibody. N-terminal. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC36A1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SLC36A1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z2H8
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC36A1 antibody - N-terminal (ab189441)
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human kidney sections labeling SLC36A1 using ab189441 at 5 μg/ml.
Immunocytochemistry/ Immunofluorescence - Anti-SLC36A1 antibody - N-terminal (ab189441)
Immunofluorescent staining of NCI-460 cells labeling SLC36A1 using ab189441 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Flow Cytometry (Intracellular) - Anti-SLC36A1 antibody - N-terminal (ab189441)
Intracellular flow cytometric analysis of NCI-460 cells (right histogram) compared to a negative control cell (left histogram) labeling SLC36A1 using ab189441 at 1/10 dilution. FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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