Anti-SLC39A14/ZIP-14 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC39A14/ZIP-14 antibody (AB106568)

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ZIP14 antibody (ab106568) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC39A14/ZIP-14 antibody (AB106568)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ZIP14 antibody (ab106568) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC39A14/ZIP-14 antibody (AB106568)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-ZIP14 antibody (ab106568) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• WB

Lab

Western blot - Anti-SLC39A14/ZIP-14 antibody (AB106568)

False colour image of Western blot : Anti-SLC39A14/ZIP-14 antibody staining at 1 μg/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab106568 was shown to bind specifically to SLC39A14/ZIP-14. A band was observed at 68 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in SLC39A14 knockout cell line ab266126 (knockout cell lysate ab258683). To generate this image, wild-type and SLC39A14 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^®20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-SLC39A14/ZIP-14 antibody (ab106568) at 1 µg/mL

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

SLC39A14 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human SLC39A14 (ZIP-14) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-slc39a14-zip-14-knockout-hek-293t-cell-line-ab266126'>ab266126</a>)

Lane 2:

Western blot - Human SLC39A14 (ZIP-14) knockout HEK-293T cell lysate (<a href='/en-us/products/cell-lysates/human-slc39a14-zip-14-knockout-hek-293t-cell-lysate-ab258683'>ab258683</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Predicted band size: 54 kDa

Observed band size: 68 kDa

false

• WB

Supplier Data

Western blot - Anti-SLC39A14/ZIP-14 antibody (AB106568)

1 h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-SLC39A14/ZIP-14 antibody (ab106568) at 2 µg/mL

Lane 1:

Human liver tissue lysate at 10 µg

Lane 2:

Mouse kidney tissue lysate at 10 µg

Lane 3:

Rat kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 54 kDa

false