Rabbit Polyclonal SLC39A14/ZIP-14 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SLC39A14.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SLC39A14. The exact immunogen used to generate this antibody is proprietary information. Database link Q15043
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Cow | Predicted |
| Dog | Predicted |
| Gorilla | Predicted |
| Hamster | Predicted |
| Monkey | Predicted |
| Orangutan | Predicted |
| Pig | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-7.50000 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Hamster, Cow, Dog, Pig, Monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Electroneutral transporter of the plasma membrane mediating the cellular uptake of the divalent metal cations zinc, manganese and iron that are important for tissue homeostasis, metabolism, development and immunity (PubMed:15642354, PubMed:27231142, PubMed:29621230). Functions as an energy-dependent symporter, transporting through the membranes an electroneutral complex composed of a divalent metal cation and two bicarbonate anions (By similarity). Beside these endogenous cellular substrates, can also import cadmium a non-essential metal which is cytotoxic and carcinogenic (By similarity). Controls the cellular uptake by the intestinal epithelium of systemic zinc, which is in turn required to maintain tight junctions and the intestinal permeability (By similarity). Modifies the activity of zinc-dependent phosphodiesterases, thereby indirectly regulating G protein-coupled receptor signaling pathways important for gluconeogenesis and chondrocyte differentiation (By similarity). Regulates insulin receptor signaling, glucose uptake, glycogen synthesis and gluconeogenesis in hepatocytes through the zinc-dependent intracellular catabolism of insulin (PubMed:27703010). Through zinc cellular uptake also plays a role in the adaptation of cells to endoplasmic reticulum stress (By similarity). Major manganese transporter of the basolateral membrane of intestinal epithelial cells, it plays a central role in manganese systemic homeostasis through intestinal manganese uptake (PubMed:31028174). Also involved in manganese extracellular uptake by cells of the blood-brain barrier (PubMed:31699897). May also play a role in manganese and zinc homeostasis participating in their elimination from the blood through the hepatobiliary excretion (By similarity). Also functions in the extracellular uptake of free iron. May also function intracellularly and mediate the transport from endosomes to cytosol of iron endocytosed by transferrin (PubMed:20682781). Plays a role in innate immunity by regulating the expression of cytokines by activated macrophages (PubMed:23052185).
Alternative names
KIAA0062, ZIP14, SLC39A14, Metal cation symporter ZIP14, LIV-1 subfamily of ZIP zinc transporter 4, Solute carrier family 39 member 14, Zrt- and Irt-like protein 14, LZT-Hs4, ZIP-14
Recommended products
Rabbit Polyclonal SLC39A14/ZIP-14 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SLC39A14.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SLC39A14. The exact immunogen used to generate this antibody is proprietary information. Database link Q15043
- Purification technique
- Affinity purification Immunogen
- Specificity
BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SLC39A14 also known as ZIP14 is a transporter protein that facilitates the import of zinc manganese and iron into cells. It belongs to the Zrt/Irt-like protein (ZIP) family and weighs approximately 52 kDa. Researchers have observed its expression in various tissues including the liver pancreas and kidney. It can also be found in prostate tissue. Through its role in metal ion transport SLC39A14 plays a significant function in maintaining cellular trace metal homeostasis.
- Biological function summary
The activity of SLC39A14 impacts numerous cellular processes by modulating intracellular metal concentrations. Its inclusion in the ZIP transporter family ties it to the regulation of metal ion balance critical for enzyme activities metabolic pathways and gene expression. While SLC39A14 does not form a complex with other proteins it operates in concert with other metal ion transporters ensuring proper ion distribution across cellular compartments.
- Pathways
SLC39A14 influences metal transport pathways that are essential for maintaining metal homeostasis and signal transduction. It closely interacts with other metal transporters like SLC39A8 which cooperates to transport manganese and zinc. These pathways are essential components in ensuring proper enzyme functionality and cellular responses. SLC39A14’s role in import affects the PI3K-Akt signaling pathway by modulating the necessary metal cofactors for key enzymes.
- Associated diseases and disorders
Defects or imbalances in SLC39A14 function associate with disturbances in metal homeostasis leading to conditions like hypermanganesemia and liver pathology. Its activity affects the metabolism of manganese and aberrations can result in the accumulation of manganese impacting nervous system functions. SLC39A14 also connects to the protein ceruloplasmin in the context of iron metabolism disorders. Inefficient regulation or mutation of SLC39A14 can exacerbate disease progression in iron overload conditions highlighting its role as a potential therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC39A14/ZIP-14 antibody (ab219174)
Immunohistochemical analysis of formalin-fixed paraffin-embedded human kidney tissue sections labeling SLC39A14/ZIP-14 with ab219174 at 7.5 μg/mL.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC39A14/ZIP-14 antibody (ab219174)
Immunohistochemical analysis of formalin-fixed paraffin-embedded human prostate tissue sections labeling SLC39A14/ZIP-14 with ab219174 at 7.5 μg/mL.
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