AB241205

Anti-SLC39A6/ZIP-6 antibody

Name: Anti-SLC39A6/ZIP-6 antibody (ab241205) | Abcam
Brand: Abcam Limited
SKU: AB241205
Price: 510 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal SLC39A6/ZIP-6 antibody. Suitable for WB, IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC39A6 aa 100-150.

View Alternative Names

LIV1, ZIP6, SLC39A6, Zinc transporter ZIP6, Estrogen-regulated protein LIV-1, Solute carrier family 39 member 6, Zrt- and Irt-like protein 6, ZIP-6

2 Images

Supplier Data

Immunoprecipitation - Anti-SLC39A6/ZIP-6 antibody (AB241205)

SLC39A6/ZIP-6 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinom) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241205 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241205 at 0.1 μg/ml.

Lane 1 : ab241205 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-SLC39A6/ZIP-6 antibody (ab241205)

Predicted band size: 85 kDa

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Supplier Data

Western blot - Anti-SLC39A6/ZIP-6 antibody (AB241205)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-SLC39A6/ZIP-6 antibody (ab241205) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 15 µg

Predicted band size: 85 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SLC39A6 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q13433

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC39A6 also known as ZIP-6 functions as a zinc transporter involved in importing zinc into cells from the extracellular environment. This protein is part of the ZIP family and has a mass of approximately 93 kDa. ZIP-6 is frequently expressed in tissues such as liver kidney and various epithelial cells. ZIP transporters like ZIP-6 play an important role in zinc homeostasis by facilitating cellular uptake of this essential trace element which impacts many cellular processes.

Biological function summary

ZIP-6 actively regulates intracellular zinc levels essential for many cellular functions like growth and development. It exists independently and does not directly form a part of a larger complex. ZIP-6 enhances zinc-dependent signaling pathways and is necessary for different cellular tasks like gene expression and enzyme activity. By controlling zinc entry into cells ZIP-6 supports several zinc-driven biological processes impacting cellular metabolism and survival.

Pathways

Researchers link ZIP-6 to the JAK-STAT signaling and MAPK pathways. In these pathways zinc acts as a necessary cofactor for numerous enzymes and transcription factors. ZIP-6's zinc transport activity connects with proteins such as STAT3 in the JAK-STAT pathway influencing cell proliferation and differentiation. ZIP-6's regulation of zinc concentrations affects the MAPK pathway which includes proteins like ERK1/2 highlighting its involvement in signal transduction and cellular response to stimuli.

ZIP-6 plays an essential role in cancer progression and immune system dysfunctions. Overexpression of ZIP-6 associates with breast cancer where it can promote proliferation and metastasis by affecting zinc-dependent pathways. ZIP-6 activity relates also to immune-related disorders influencing the inflammatory response through interactions with NF-kappaB. Changes in the activity or expression of ZIP-6 alter zinc homeostasis adding complexity to its disease-related impact.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Zinc-influx transporter which plays a role in zinc homeostasis and in the induction of epithelial-to-mesenchymal transition (EMT) (PubMed : 12839489, PubMed : 18272141, PubMed : 21422171, PubMed : 23919497, PubMed : 27274087, PubMed : 34394081). When associated with SLC39A10, the heterodimer formed by SLC39A10 and SLC39A6 mediates cellular zinc uptake to trigger cells to undergo epithelial- to-mesenchymal transition (EMT) (PubMed : 27274087). The SLC39A10-SLC39A6 heterodimer also controls NCAM1 phosphorylation and its integration into focal adhesion complexes during EMT (By similarity). Zinc influx inactivates GSK3B, enabling unphosphorylated SNAI1 in the nucleus to down-regulate adherence genes such as CDH1, causing loss of cell adherence (PubMed : 23919497). In addition, the SLC39A10-SLC39A6 heterodimer plays an essentiel role in initiating mitosis by importing zinc into cells to initiate a pathway resulting in the onset of mitosis (PubMed : 32797246). Participates in the T-cell receptor signaling regulation by mediating cellular zinc uptake into activated lymphocytes (PubMed : 21422171, PubMed : 30552163, PubMed : 34394081). Regulates the zinc influx necessary for proper meiotic progression to metaphase II (MII) that allows the oocyte-to-egg transition (PubMed : 25143461).

See full target information SLC39A6

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2022:8825784 PubMed35281474

2022

BMSC-EV-derived lncRNA NORAD Facilitates Migration, Invasion, and Angiogenesis in Osteosarcoma Cells by Regulating CREBBP Delivery of miR-877-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Dapeng Feng,Zhengwei Li,Liang Yang,Haidong Liang,Hongtao He,Lin Liu,Wei Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLC39A6/ZIP-6 antibody

Immunoprecipitation - Anti-SLC39A6/ZIP-6 antibody (AB241205)

Western blot - Anti-SLC39A6/ZIP-6 antibody (AB241205)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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