AB243938

Anti-SLC39A6/ZIP-6 antibody [SP300] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SLC39A6/ZIP-6 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.

View Alternative Names

LIV1, ZIP6, SLC39A6, Zinc transporter ZIP6, Estrogen-regulated protein LIV-1, Solute carrier family 39 member 6, Zrt- and Irt-like protein 6, ZIP-6

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC39A6/ZIP-6 antibody [SP300] - BSA and Azide free (AB243938)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC39A6/ZIP-6 antibody [SP300] - BSA and Azide free (AB243938)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for SLC39A6/ZIP-6 using ab227696 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227696).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC39A6/ZIP-6 antibody [SP300] - BSA and Azide free (AB243938)

Formalin-fixed, paraffin-embedded human uterus tissue stained for SLC39A6/ZIP-6 using ab227696 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227696).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC39A6/ZIP-6 antibody [SP300] - BSA and Azide free (AB243938)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling SLC39A6/ZIP-6 with ab227696 at 1/100 dilution (4.05 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227696)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP300

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Perform heat mediated antigen retrieval by boiling tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.Primary antibody incubation for 10 minutes at room temperature." }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab243938 is the carrier-free version of ab227696.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC39A6 also known as ZIP-6 functions as a zinc transporter involved in importing zinc into cells from the extracellular environment. This protein is part of the ZIP family and has a mass of approximately 93 kDa. ZIP-6 is frequently expressed in tissues such as liver kidney and various epithelial cells. ZIP transporters like ZIP-6 play an important role in zinc homeostasis by facilitating cellular uptake of this essential trace element which impacts many cellular processes.

Biological function summary

ZIP-6 actively regulates intracellular zinc levels essential for many cellular functions like growth and development. It exists independently and does not directly form a part of a larger complex. ZIP-6 enhances zinc-dependent signaling pathways and is necessary for different cellular tasks like gene expression and enzyme activity. By controlling zinc entry into cells ZIP-6 supports several zinc-driven biological processes impacting cellular metabolism and survival.

Pathways

Researchers link ZIP-6 to the JAK-STAT signaling and MAPK pathways. In these pathways zinc acts as a necessary cofactor for numerous enzymes and transcription factors. ZIP-6's zinc transport activity connects with proteins such as STAT3 in the JAK-STAT pathway influencing cell proliferation and differentiation. ZIP-6's regulation of zinc concentrations affects the MAPK pathway which includes proteins like ERK1/2 highlighting its involvement in signal transduction and cellular response to stimuli.

ZIP-6 plays an essential role in cancer progression and immune system dysfunctions. Overexpression of ZIP-6 associates with breast cancer where it can promote proliferation and metastasis by affecting zinc-dependent pathways. ZIP-6 activity relates also to immune-related disorders influencing the inflammatory response through interactions with NF-kappaB. Changes in the activity or expression of ZIP-6 alter zinc homeostasis adding complexity to its disease-related impact.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Zinc-influx transporter which plays a role in zinc homeostasis and in the induction of epithelial-to-mesenchymal transition (EMT) (PubMed : 12839489, PubMed : 18272141, PubMed : 21422171, PubMed : 23919497, PubMed : 27274087, PubMed : 34394081). When associated with SLC39A10, the heterodimer formed by SLC39A10 and SLC39A6 mediates cellular zinc uptake to trigger cells to undergo epithelial- to-mesenchymal transition (EMT) (PubMed : 27274087). The SLC39A10-SLC39A6 heterodimer also controls NCAM1 phosphorylation and its integration into focal adhesion complexes during EMT (By similarity). Zinc influx inactivates GSK3B, enabling unphosphorylated SNAI1 in the nucleus to down-regulate adherence genes such as CDH1, causing loss of cell adherence (PubMed : 23919497). In addition, the SLC39A10-SLC39A6 heterodimer plays an essentiel role in initiating mitosis by importing zinc into cells to initiate a pathway resulting in the onset of mitosis (PubMed : 32797246). Participates in the T-cell receptor signaling regulation by mediating cellular zinc uptake into activated lymphocytes (PubMed : 21422171, PubMed : 30552163, PubMed : 34394081). Regulates the zinc influx necessary for proper meiotic progression to metaphase II (MII) that allows the oocyte-to-egg transition (PubMed : 25143461).

See full target information SLC39A6

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLC39A6/ZIP-6 antibody [SP300] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC39A6/ZIP-6 antibody [SP300] - BSA and Azide free (AB243938)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC39A6/ZIP-6 antibody [SP300] - BSA and Azide free (AB243938)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC39A6/ZIP-6 antibody [SP300] - BSA and Azide free (AB243938)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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