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AB251402

Anti-SLC45A3 antibody [EPR18068] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SLC45A3 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PCANAP6, PRST, SLC45A3, Solute carrier family 45 member 3, Prostate cancer-associated protein 6, Prostein

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR18068] - BSA and Azide free (AB251402)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR18068] - BSA and Azide free (AB251402)

This data was developed using ab203827, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SLC45A3 with ab203827 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR18068] - BSA and Azide free (AB251402)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR18068] - BSA and Azide free (AB251402)

This data was developed using ab203827, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human prostatic carcinoma tissue labeling SLC45A3 with ab203827 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human prostatic carcinoma tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR18068] - BSA and Azide free (AB251402)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR18068] - BSA and Azide free (AB251402)

This data was developed using ab203827, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling SLC45A3 with ab203827 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR18068] - BSA and Azide free (AB251402)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR18068] - BSA and Azide free (AB251402)

This data was developed using ab203827, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling SLC45A3 with ab203827 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human prostatic hyperplasia tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18068

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab251402 is the carrier-free version of ab203827.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC45A3 also known as Prostate Cancer Associated Transcript 6 (PCAT6) is a member of the solute carrier family that functions primarily as a transporter. This protein has a mass of approximately 51 kDa. It is primarily expressed in the prostate but also found in other tissues though at lower levels. SLC45A3 is involved in transporting certain solutes which is important for maintaining cellular homeostasis.
Biological function summary

This transporter plays a critical role in regulating pH and solute concentration within the cells. SLC45A3 does not appear to belong to a large protein complex but it interacts with elements of the intracellular environment that modulate its activity. Its solute transportation role affects a range of cellular processes impacting overall cell health and function.

Pathways

SLC45A3 operates within the cellular homeostasis and metabolic pathways. It closely relates to the acid-base balance pathway ensuring that cells maintain optimal internal conditions. Additionally its function can link to the androgen receptor signaling pathway an important pathway in prostate tissue where it may interact indirectly with other transport proteins that assist in maintaining homeostatic balance.

SLC45A3 is significantly connected to prostate cancer and may also have implications in other types of cancer. It associates with the androgen receptor which plays a major role in the progression of prostate cancer. Understanding the dynamics of SLC45A3 can help in comprehending its role in these diseases potentially offering targets for new therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Proton-associated sucrose transporter. May be able to transport also glucose and fructose.
See full target information SLC45A3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:3596 PubMed34155205

2021

ERRγ enhances cardiac maturation with T-tubule formation in human iPSC-derived cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Kenji Miki,Kohei Deguchi,Misato Nakanishi-Koakutsu,Antonio Lucena-Cacace,Shigeru Kondo,Yuya Fujiwara,Takeshi Hatani,Masako Sasaki,Yuki Naka,Chikako Okubo,Megumi Narita,Ikue Takei,Stephanie C Napier,Tsukasa Sugo,Sachiko Imaichi,Taku Monjo,Tatsuya Ando,Norihisa Tamura,Kenichi Imahashi,Tomoyuki Nishimoto,Yoshinori Yoshida
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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