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AB137065

Anti-SLC45A3 antibody [EPR4795(2)]

5

(1 Review)

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(5 Publications)

Anti-SLC45A3 antibody [EPR4795(2)] (ab137065) is a rabbit monoclonal antibody detecting SLC45A3 in Western Blot, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

PCANAP6, PRST, SLC45A3, Solute carrier family 45 member 3, Prostate cancer-associated protein 6, Prostein

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (AB137065)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (AB137065)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue sections labeling SLC45A3 with purified ab137065 at 1/1000 dilution (0.128 µg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (AB137065)
  • IHC-P

AbReview54643****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (AB137065)

Immunohistochemical analysis of human prostate tissue sections labelling SLC45A3 with ab137065, unpurified, at a dilution of 1/2000. The sections were fixed with 10% NBF. The secondary antibody used was HRP conjugated goat anti rabbit IgG. Antigen retrieval was heat mediated using Tris/EDTA pH 9.0.

This image is courtesy of an abreview submitted by Faye Doherty

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (AB137065)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (AB137065)

Immunohistochemical analysis of paraffin-embedded Human prostatic adenocarcinoma tissue labelling SLC45A3 with as137065, unpurified, at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (AB137065)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (AB137065)

Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labelling SLC45A3 with ab137065, unpurified, at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-SLC45A3 antibody [EPR4795(2)] (AB137065)
  • WB

Unknown

Western blot - Anti-SLC45A3 antibody [EPR4795(2)] (AB137065)

Blocking/Diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065) at 1/1000 dilution

All lanes:

Human prostate lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 59 kDa

Observed band size: 55 kDa

false

  • Carrier free

    Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free

  • 660 APC

    APC Anti-SLC45A3 antibody [EPR4795(2)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-SLC45A3 antibody [EPR4795(2)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-SLC45A3 antibody [EPR4795(2)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-SLC45A3 antibody [EPR4795(2)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-SLC45A3 antibody [EPR4795(2)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-SLC45A3 antibody [EPR4795(2)]

  • 578 PE

    PE Anti-SLC45A3 antibody [EPR4795(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4795(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p><strong>For unpurifed use at 1/100 - 1/250</strong></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-SLC45A3 antibody [EPR4795(2)] (ab137065) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of SLC45A3?
Anti-SLC45A3 [EPR4795(2)] (ab137065) specifically detects a band for SLC45A3 (UniProt: Q96JT2) at a molecular weight of 59kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR4795(2)] also available for your convenience: ab137065, Carrier free - ab242056, PE - ab317904, APC - ab317943, Alexa Fluor® 488 - ab317984, Alexa Fluor® 594 - ab318026, Alexa Fluor® 555 - ab318068, Alexa Fluor® 647 - ab318109, Alexa Fluor® 750 - ab321108

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC45A3 also known as Prostate Cancer Associated Transcript 6 (PCAT6) is a member of the solute carrier family that functions primarily as a transporter. This protein has a mass of approximately 51 kDa. It is primarily expressed in the prostate but also found in other tissues though at lower levels. SLC45A3 is involved in transporting certain solutes which is important for maintaining cellular homeostasis.
Biological function summary

This transporter plays a critical role in regulating pH and solute concentration within the cells. SLC45A3 does not appear to belong to a large protein complex but it interacts with elements of the intracellular environment that modulate its activity. Its solute transportation role affects a range of cellular processes impacting overall cell health and function.

Pathways

SLC45A3 operates within the cellular homeostasis and metabolic pathways. It closely relates to the acid-base balance pathway ensuring that cells maintain optimal internal conditions. Additionally its function can link to the androgen receptor signaling pathway an important pathway in prostate tissue where it may interact indirectly with other transport proteins that assist in maintaining homeostatic balance.

SLC45A3 is significantly connected to prostate cancer and may also have implications in other types of cancer. It associates with the androgen receptor which plays a major role in the progression of prostate cancer. Understanding the dynamics of SLC45A3 can help in comprehending its role in these diseases potentially offering targets for new therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Proton-associated sucrose transporter. May be able to transport also glucose and fructose.
See full target information SLC45A3
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadq1575 PubMed40184463

2025

Atf3 controls transitioning in female mitochondrial cardiomyopathy as identified by spatial and single-cell transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Tasneem Qaqorh,Yusuke Takahashi,Kohei Sameshima,Kentaro Otani,Issei Yazawa,Yuya Nishida,Kohei Tonai,Yoshitaka Fujihara,Mizuki Honda,Shinya Oki,Yasuyuki Ohkawa,David R Thorburn,Ann E Frazier,Atsuhito Takeda,Yoshihiko Ikeda,Heima Sakaguchi,Takuya Watanabe,Norihide Fukushima,Yasumasa Tsukamoto,Naomasa Makita,Osamu Yamaguchi,Kei Murayama,Akira Ohtake,Yasushi Okazaki,Takanari Kimura,Hisakazu Kato,Hijiri Inoue,Ken Matsuoka,Seiji Takashima,Yasunori Shintani

Diagnostic pathology 19:12 PubMed38218896

2024

Prostein expression in human tumors: a tissue microarray study on 19,202 tumors from 152 different Tumor entities.

Applications

Unspecified application

Species

Unspecified reactive species

Florian Viehweger,Carola Böcker,Sören Weidemann,Morton Freytag,Anne Menz,Franziska Büscheck,Andreas M Luebke,Devita Putri,Martina Kluth,Claudia Hube-Magg,Andrea Hinsch,Maximilian Lennartz,Florian Lutz,Viktor Reiswich,Doris Höflmayer,Christoph Fraune,Katharina Möller,Christian Bernreuther,Patrick Lebok,Guido Sauter,Stefan Steurer,David Dum,Andreas H Marx,Ronald Simon,Till Krech,Till S Clauditz,Frank Jacobsen,Natalia Gorbokon,Eike Burandt,Sarah Minner,Simon Kind

Translational stroke research 15:556-571 PubMed36913120

2023

SLC45A3 Serves as a Potential Therapeutic Biomarker to Attenuate White Matter Injury After Intracerebral Hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhang,Hanhai Zeng,Feiyang Lou,Xiaoxiao Tan,Xiaotong Zhang,Gao Chen

Molecular cancer 19:147 PubMed33032611

2020

Identification of a distinct luminal subgroup diagnosing and stratifying early stage prostate cancer by tissue-based single-cell RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoshi Ma,Jinan Guo,Kaisheng Liu,Lipeng Chen,Dale Liu,Shaowei Dong,Jinquan Xia,Qiaoyun Long,Yongjian Yue,Pan Zhao,Fengyan Hu,Zhangang Xiao,Xinghua Pan,Kefeng Xiao,Zhiqiang Cheng,Zunfu Ke,Zhe-Sheng Chen,Chang Zou

Respiratory research 21:186 PubMed32678044

2020

In vivo miR-138-5p inhibition alleviates monocrotaline-induced pulmonary hypertension and normalizes pulmonary KCNK3 and SLC45A3 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Hélène Le Ribeuz,Audrey Courboulin,Maria-Rosa Ghigna,Mélanie Lambert,Aurélie Hautefort,Marc Humbert,David Montani,Sylvia Cohen-Kaminsky,Frédéric Perros,Fabrice Antigny
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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