Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free (AB242056)

Immunohistochemical analysis of paraffin-embedded Human prostatic adenocarcinoma tissue labelling SLC45A3 with as137065 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137065).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free (AB242056)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue sections labeling SLC45A3 with purified ab137065 at 1/1000 dilution (0.128 μg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137065).

• IHC-P

AbReview54643****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free (AB242056)

Immunohistochemical analysis of human prostate tissue sections labelling SLC45A3 with ab137065 at a dilution of 1/2000. The sections were fixed with 10% NBF. The secondary antibody used was HRP conjugated goat anti rabbit IgG. Antigen retrieval was heat mediated using Tris/EDTA pH 9.0.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137065).

This image is courtesy of an abreview submitted by Faye Doherty

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free (AB242056)

Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labelling SLC45A3 with ab137065 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137065).

• WB

Unknown

Western blot - Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free (AB242056)

Blocking/Diluting buffer : 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137065).

All lanes:

Western blot - Anti-SLC45A3 antibody [EPR4795(2)] (<a href='/en-us/products/primary-antibodies/slc45a3-antibody-epr47952-ab137065'>ab137065</a>) at 1/1000 dilution

All lanes:

Human prostate lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 59 kDa

Observed band size: 55 kDa

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