AB243826

Anti-SLC46A3 antibody

Name: Anti-SLC46A3 antibody (ab243826) | Abcam
Brand: Abcam Limited
SKU: AB243826
Price: 630 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal SLC46A3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SLC46A3 aa 1-100.

View Alternative Names

FKSG16, SLC46A3, Lysosomal proton-coupled steroid conjugate and bile acid symporter SLC46A3, Solute carrier family 46 member 3

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC46A3 antibody (AB243826)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC46A3 antibody (AB243826)

Immunohistochemistry analysis of human fallopian tube tissue labeling SLC46A3 with ab243826 at 1 : 200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC46A3 antibody (AB243826)

Immunohistochemistry analysis of human liver tissue labeling SLC46A3 with ab243826 at 1 : 200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC46A3 antibody (AB243826)

Immunohistochemistry analysis of human testis tissue labeling SLC46A3 with ab243826 at 1 : 200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC46A3 antibody (AB243826)

Immunohistochemistry analysis of human small intestine tissue labeling SLC46A3 with ab243826 at 1 : 200 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-SLC46A3 antibody (AB243826)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SLC46A3 antibody (AB243826)

PFA fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling SLC46A3 using ab243826 at 4 μg/ml (green) in ICC/IF.

Supplier Data

Western blot - Anti-SLC46A3 antibody (AB243826)

All lanes:

Western blot - Anti-SLC46A3 antibody (ab243826) at 0.4 µg/mL

Lane 1:

RT4 (Human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (Human brain glioma cell line) cell lysate

Lane 3:

Human plasma (IgG/HSA depleted)

Lane 4:

Human liver tissue lysate

Lane 5:

Human tonsil tissue lysate

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SLC46A3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q7Z3Q1

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC46A3 also known as Solute Carrier Family 46 Member 3 is a membrane protein facilitating the transport of folate and antifolate compounds across cell membranes. It weighs approximately 53 kDa. Expression of SLC46A3 occurs in several tissues with higher levels in liver and kidney tissues. It plays an essential role in the transport of necessary nutrients and drugs inside the cell.

Biological function summary

SLC46A3 contributes to maintaining cellular homeostasis by regulating folate uptake. While not thought to be part of a larger complex it acts independently to ensure the proper concentration of intracellular folate which is critical for DNA synthesis and repair. Its ability to transport folate emphasizes its importance in maintaining cell proliferation and survival.

Pathways

SLC46A3 influences the folate metabolism pathway and impacts the pharmacokinetics of antifolate drugs such as methotrexate. It works closely with proteins like SLC19A1 another folate transporter to mediate folate and antifolate intracellular concentration. These pathways are essential for understanding drug efficacy and resistance in cancer therapy given folate's role in cell growth.

SLC46A3 alludes to a potential role in cancer and antifolate drug resistance. Its function in drug transport links it to the reduced effectiveness of chemotherapy drugs. Moreover research suggests a connection between SLC46A3 and SLC19A1 both affecting the cellular reaction to antifolate therapies in certain cancer types. Understanding SLC46A3's role could lead to improved therapeutic strategies in oncology.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Lysosomal proton-coupled steroid conjugate and bile acid transporter. Preferentially recognizes lipophilic steroid conjugates or bile acis as endogenous substrates and seems to mediate escape from lysosomes to the cytoplasm (PubMed : 36741448). Modulates hepatic cytosolic copper homeostasis, maybe acting as a lysosomal copper transporter and sequestering copper ions in the lysosome (By similarity). Transports catabolites of non-cleavable antibody-drug conjugates from the lysosome to the cytoplasm (PubMed : 26631267, PubMed : 30131388, PubMed : 36741448). Delivers pathogen-associated molecular patterns to cytosolic pattern recognition receptors as part of the innate immune response to microbes. Selectively transports bacterial muramyl dipeptide (MDP) into the cytosol for recognition by NOD2, triggering inflammatory responses (By similarity). Likely acts as a redundant importer of cyclic GMP-AMP dinucleotides (cGAMPs) in monocyte and macrophage cell lineages (PubMed : 34235268). The transport mechanism, its electrogenicity and stoichiometry remain to be elucidated (Probable).

See full target information SLC46A3

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular histology 56:144 PubMed40285932

2025

Identification of the oncogenic role of centromere protein M in non-small cell lung cancer via CDC20/MYBL2/Wnt signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Wu,Jun Li,Haoyu Wang,Xu Chang,Qinglong Kong

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLC46A3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC46A3 antibody (AB243826)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC46A3 antibody (AB243826)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC46A3 antibody (AB243826)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC46A3 antibody (AB243826)

Immunocytochemistry/ Immunofluorescence - Anti-SLC46A3 antibody (AB243826)

Western blot - Anti-SLC46A3 antibody (AB243826)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Secondary Antibodies

Sample Prep & Detection Kits

Primary Antibodies

Proteins & Peptides

Product promise