Rabbit Polyclonal SLC4A4/NBC antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC4A4 aa 1-250.
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- Frontiers in cellular neuroscience 17:12534242023K/Cl cotransporter 2 (KCC2) and Na/ cotransporter 1 (NBCe1) interaction modulates profile of KCC2 phosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbhishek Pethe,Mira Hamze,Marina Giannaki,Bernd Heimrich,Igor Medina,Anna-Maria Hartmann,Eleni RoussaPubMed 37881493
- Frontiers in cell and developmental biology 11:11616672023Identification and verification of a prognostic signature based on a miRNA-mRNA interaction pattern in colon adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiwu Zhao,Haosheng Li,Wenchang Li,Zichao Guo,Wenqing Jia,Shuiyu Xu,Sixia Chen,Xiaonan Shen,Changgang WangPubMed 37745305
- Nature cancer 3:1464-14832022Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFederica Cappellesso,Marie-Pauline Orban,Niranjan Shirgaonkar,Emanuele Berardi,Jens Serneels,Marie-Aline Neveu,Daria Di Molfetta,Francesca Piccapane,Rosa Caroppo,Lucantonio Debellis,Tessa Ostyn,Nicolas Joudiou,Lionel Mignion,Elena Richiardone,Bénédicte F Jordan,Bernard Gallez,Cyril Corbet,Tania Roskams,Ramanuj DasGupta,Sabine Tejpar,Mario Di Matteo,Daniela Taverna,Stephan J Reshkin,Baki Topal,Federico Virga,Massimiliano MazzonePubMed 36522548
- EMBO reports 22:e532002021Regional heterogeneity of astrocyte morphogenesis dictated by the formin protein, Daam2, modifies circuit function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuyeon Jo,Junsung Woo,Carlo D Cristobal,Jong Min Choi,Chih-Yen Wang,Qi Ye,Joshua A Smith,Kevin Ung,Gary Liu,Diego Cortes,Sung Yun Jung,Benjamin R Arenkiel,Hyun Kyoung LeePubMed 34633730
- Journal of inflammation research 14:3809-38212021Identification and Validation of a Novel Inflammatory Response-Related Gene Signature for the Prognosis of Colon Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYichao Liang,Xin Wu,Qi Su,Yujie Liu,Hong XiaoPubMed 34408464
- Journal of Cancer 12:5439-54532021SLC34A2 Up-regulation And SLC4A4 Down-regulation Correlates With Invasion, Metastasis, And The MAPK Signaling Pathway In Papillary Thyroid Carcinomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFengyan Huang,Haitao Wang,Juan Xiao,Chunchun Shao,Yong Zhou,Wei Cong,Maosong Gong,Jingfu Sun,Liqun Shan,Zhanyu Hao,Lihua Wang,Shouluan Ding,Zhigang Yu,Jianing Liu,Hongying JiaPubMed 34405007
- Kidney international 97:304-3152019Defective bicarbonate reabsorption in Kir4.2 potassium channel deficient mice impairs acid-base balance and ammonia excretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYohan Bignon,Laurent Pinelli,Nadia Frachon,Olivier Lahuna,Lucile Figueres,Pascal Houillier,Stéphane Lourdel,Jacques Teulon,Marc PaulaisPubMed 31870500
- Bulletin of experimental biology and medicine 168:219-2232019Molecular Targets of Natriuretic Action of Prolactin in the Rat Model of Cholestasis of Pregnancy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesP A Abramicheva et. al.PubMed 31776946
- Molecules (Basel, Switzerland) 24:2019Drug Repurposing as an Antitumor Agent: Disulfiram-Mediated Carbonic Anhydrase 12 and Anion Exchanger 2 Modulation to Inhibit Cancer Cell Migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSoyoung Hwang et. al.PubMed 31546841
- Thyroid : official journal of the American Thyroid 29:290-3012019Expression, Localization, and Regulation of the Sodium Bicarbonate Cotransporter NBCe1 in the Thyroid.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMyrna Virreira et. al.PubMed 30526387
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 49.95% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SLC4A4 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6R1
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.
Alternative names
NBC, NBC1, NBCE1, SLC4A4, Electrogenic sodium bicarbonate cotransporter 1, Sodium bicarbonate cotransporter, Na(+)/HCO3(-) cotransporter, Solute carrier family 4 member 4, kNBC1
Recommended products
Rabbit Polyclonal SLC4A4/NBC antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC4A4 aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 49.95% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SLC4A4 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6R1
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SLC4A4 also known as NBC (electrogenic Na+/bicarbonate cotransporter) is a protein that functions in the active transport of bicarbonate ions across cell membranes coupled with sodium ions. This cotransporter plays an essential role in maintaining the acid-base balance and cellular pH homeostasis. The SLC4A4 protein has a molecular mass of approximately 121 kDa. It expresses in various tissues with high levels found in the kidney and pancreas. Its activity is critical for efficient transport processes in epithelial cells contributing to the overall ion balance within these organs.
- Biological function summary
The activity of SLC4A4/NBC influences several physiological processes. This protein helps regulate bicarbonate reabsorption in renal proximal tubules which is fundamental for the body's buffering capacity. SLC4A4 can operate within complex systems involving other transport mechanisms to efficiently maintain proper ion concentrations and pH balance. It associates with proteins like carbonic anhydrase which facilitates the rapid interconversion of carbon dioxide and bicarbonate enhancing the efficiency of its transport function.
- Pathways
SLC4A4/NBC integrates into several key biological pathways that maintain bicarbonate and acid-base equilibrium. One such pathway is the renal tubular bicarbonate reclamation where it collaborates closely with proteins such as NHE3 (sodium hydrogen exchanger 3) to ensure sodium and bicarbonate absorption. Another pathway is the pancreatic bicarbonate secretion where its role complements secretor proteins to assimilate bicarbonates essential for neutralizing gastric acids.
- Associated diseases and disorders
SLC4A4/NBC correlates with significant clinical conditions affecting bicarbonate transport. Mutations or dysfunction in this transporter relate to proximal renal tubular acidosis a disorder characterized by impaired acid-base regulation in kidneys. Additionally it associates with migraine disorders where alterations in pH and ion flow might play a role. Through these conditions SLC4A4 may connect with other bicarbonate transporters such as SLC4A7 to provide insights into pathophysiological mechanisms involving ionic imbalances.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC4A4/NBC antibody (ab187511)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labelling SLC4A4/NBC with ab187511 at 1/200. Magnification: 400x.
Western blot - Anti-SLC4A4/NBC antibody (ab187511)
All lanes: Western blot - Anti-SLC4A4/NBC antibody (ab187511) at 1/500 dilution
Lane 1: PC3 cell extract
Lane 2: A549 cell extract
Developed using the ECL technique.
Predicted band size: 121 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC4A4/NBC antibody (ab187511)
Immunohistochemical analysis of paraffin embedded mouse kidney tissue labeling SLC4A4/NBC with ab187511 at 1:100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC4A4/NBC antibody (ab187511)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling SLC4A4/NBC with ab187511 at 1/200. Magnification: 400x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC4A4/NBC antibody (ab187511)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue labelling SLC4A4/NBC with ab187511 at 1/200. Magnification: 400x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC4A4/NBC antibody (ab187511)
Immunohistochemical analysis of paraffin embedded rat kidney tissue labeling SLC4A4/NBC with ab187511 at 1:100 dilution.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-SLC4A4/NBC antibody (ab187511)
SLC4A4/NBC western blot using anti-SLC4A4/NBC antibody ab187511. Publication image and figure legend from Hwang, S., Shin, D. M., et al., 2019, Molecules, PubMed 31546841.
ab187511 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab187511 please see the product overview.
Surface protein expression of AE2 protein was decreased by DSF treatment. (A) Analysis of AE2 mRNA expression at rising concentrations from 0.1–4 μM DSF for 48 h (n = 5). (B) Protein expression of AE2 at indicated DSF concentration. (C) Analysis of relative AE2 intensity (n = 3, * p < 0.05, *** p < 0.001). (D) Immunostained images of AE2 (red), nucleus (DAPI, blue) and merge of cell morphology (TPM-T, gray) with and without DSF treatment. The scale bars represent 20 μm. (E) The surface expression of AE2 after treatment with 2 μM DSF. The β-actin and input AE2 blots were used as loading controls. (F) Analysis of relative intensity of AE2 for input and biotinylated (Bio) blot. Bars represent mean ± SEM (n = 3, ** p < 0.01). (G) Protein expressions of NBCe1 and NBCn1. (H) Analysis of relative intensity of NBCe1 and NBCn1 blots. Bars represent mean ± SEM. (I) Protein expressions of LC3B and p62. The β-actin blot was used as a loading control. (J) Analysis of relative intensity of LC3B and p62 blots. Bars represent mean ± SEM.
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