Rabbit Polyclonal SLC4A4/NBC antibody. Suitable for IHC-P and reacts with Mouse samples. Immunogen corresponding to Synthetic Peptide within Human SLC4A4 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
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Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human SLC4A4 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6R1
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Predicted |
| Mouse | Tested |
| Rat | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.Isoform 2May have a higher activity than isoform 1.
Alternative names
Electrogenic sodium bicarbonate cotransporter 1, Sodium bicarbonate cotransporter, Na(+)/HCO3(-) cotransporter, Solute carrier family 4 member 4, kNBC1, NBC, NBC1, SLC4A4, NBCE1
Recommended products
Rabbit Polyclonal SLC4A4/NBC antibody. Suitable for IHC-P and reacts with Mouse samples. Immunogen corresponding to Synthetic Peptide within Human SLC4A4 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
Alternative names
Electrogenic sodium bicarbonate cotransporter 1, Sodium bicarbonate cotransporter, Na(+)/HCO3(-) cotransporter, Solute carrier family 4 member 4, kNBC1, NBC, NBC1, SLC4A4, NBCE1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human SLC4A4 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6R1
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SLC4A4 also known as NBC (electrogenic Na+/bicarbonate cotransporter) is a protein that functions in the active transport of bicarbonate ions across cell membranes coupled with sodium ions. This cotransporter plays an essential role in maintaining the acid-base balance and cellular pH homeostasis. The SLC4A4 protein has a molecular mass of approximately 121 kDa. It expresses in various tissues with high levels found in the kidney and pancreas. Its activity is critical for efficient transport processes in epithelial cells contributing to the overall ion balance within these organs.
- Biological function summary
The activity of SLC4A4/NBC influences several physiological processes. This protein helps regulate bicarbonate reabsorption in renal proximal tubules which is fundamental for the body's buffering capacity. SLC4A4 can operate within complex systems involving other transport mechanisms to efficiently maintain proper ion concentrations and pH balance. It associates with proteins like carbonic anhydrase which facilitates the rapid interconversion of carbon dioxide and bicarbonate enhancing the efficiency of its transport function.
- Pathways
SLC4A4/NBC integrates into several key biological pathways that maintain bicarbonate and acid-base equilibrium. One such pathway is the renal tubular bicarbonate reclamation where it collaborates closely with proteins such as NHE3 (sodium hydrogen exchanger 3) to ensure sodium and bicarbonate absorption. Another pathway is the pancreatic bicarbonate secretion where its role complements secretor proteins to assimilate bicarbonates essential for neutralizing gastric acids.
- Associated diseases and disorders
SLC4A4/NBC correlates with significant clinical conditions affecting bicarbonate transport. Mutations or dysfunction in this transporter relate to proximal renal tubular acidosis a disorder characterized by impaired acid-base regulation in kidneys. Additionally it associates with migraine disorders where alterations in pH and ion flow might play a role. Through these conditions SLC4A4 may connect with other bicarbonate transporters such as SLC4A7 to provide insights into pathophysiological mechanisms involving ionic imbalances.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC4A4/NBC antibody (ab217352)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brain tissue labeling SLC4A4/NBC with ab217352 at 1/400 dilution followed by conjugation to the secondary antibody and DAB staining.
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