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AB125910

Anti-SLC4A8/NBC antibody

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(1 Publication)

Rabbit Polyclonal SLC4A8/NBC antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SLC4A8 aa 1-300.

View Alternative Names

KIAA0739, NBC, NBC3, NDCBE1, SLC4A8, Electroneutral sodium bicarbonate exchanger 1, Electroneutral Na(+)-driven Cl-HCO3 exchanger, Solute carrier family 4 member 8, k-NBC3

1 Images
Western blot - Anti-SLC4A8/NBC antibody (AB125910)
  • WB

Unknown

Western blot - Anti-SLC4A8/NBC antibody (AB125910)

Gel concentration : 7.5%

All lanes:

Western blot - Anti-SLC4A8/NBC antibody (ab125910) at 1/1000 dilution

All lanes:

Human U87-M6 whole cell lysate at 30 µg

Predicted band size: 123 kDa,78 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human SLC4A8 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q2Y0W8

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Primary Antibodies

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Primary Antibodies

AB187511

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Secondary Antibodies

AB191866

VHH Single Domain Anti-Rabbit IgG Fc (HRP)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC4A8 often referred to as NBC or the sodium bicarbonate cotransporter is an integral membrane protein with a mass of around 129 kDa. Mechanically this protein mediates the transport of sodium and bicarbonate ions across cell membranes. It operates as an electroneutral cotransporter and is predominantly located in the plasma membranes of renal and neural cells. Expression of SLC4A8 is prominent in the kidney and brain where it controls acid-base balance and ionic gradients.
Biological function summary

SLC4A8/NBC plays an essential role in maintaining intracellular pH and volume contributing to the acid-base homeostasis in the body. It does not function as part of a larger protein complex. This activity supports various cell functions including cell metabolism and neuronal activity. In kidney cells it assists in reabsorbing bicarbonate from the renal tubules which is important for the body’s pH regulation.

Pathways

SLC4A8 participates in two important biological processes: the acid-base balance and bicarbonate reabsorption pathways. These pathways include the modulation of pH levels in renal and neural tissues. It connects directly with the NHE3 protein in the kidney which also takes part in sodium and proton exchange jointly influencing sodium reabsorption and extracellular fluid volume.

SLC4A8/NBC has associations with conditions like renal tubular acidosis and epilepsy. Dysfunctional expression or mutations in the SLC4A8 gene can lead to improper bicarbonate handling causing renal tubular acidosis which affects kidney function. In neurological disorders such as epilepsy altered ion transport through this protein can disrupt neuronal activity contributing to seizure activity. The protein interacts with GABAA receptors in the brain affecting inhibitory signaling which can be connected to epilepsy.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates electroneutral sodium- and carbonate-dependent chloride-HCO3(-) exchange with a Na(+) : HCO3(-) stoichiometry of 2 : 1 (PubMed : 18577713). Plays a major role in pH regulation in neurons (By similarity). Mediates sodium reabsorption in the renal cortical collecting ducts (By similarity).
See full target information SLC4A8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:3427 PubMed37296096

2023

Muscleblind-like proteins use modular domains to localize RNAs by riding kinesins and docking to membranes.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan P Hildebrandt,Kathryn R Moss,Aleksandra Janusz-Kaminska,Luke A Knudson,Lance T Denes,Tanvi Saxena,Devi Prasad Boggupalli,Zhuangyue Li,Kun Lin,Gary J Bassell,Eric T Wang
View all publications
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Product promise

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