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AB121285

Anti-SLC51B antibody

3

(1 Review)

|

(3 Publications)

Rabbit Polyclonal SLC51B antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC51B aa 50 to C-terminus.

View Alternative Names

OSTB, SLC51B, Organic solute transporter subunit beta, OST-beta, Solute carrier family 51 subunit beta

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC51B antibody (AB121285)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC51B antibody (AB121285)

Immunohistochemical analysis of human liver tissue labeling OST beta with ab121285 at 1/50 dilution. This showes no positivity as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC51B antibody (AB121285)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC51B antibody (AB121285)

Immunohistochemical analysis of human testis tissue labeling OST beta with ab121285 at 1/50 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC51B antibody (AB121285)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC51B antibody (AB121285)

Immunohistochemical analysis of human small intestine tissue labeling OST beta with ab121285 at 1/50 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC51B antibody (AB121285)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC51B antibody (AB121285)

Immunohistochemical analysis of human kidney tissue labeling OST beta with ab121285 at 1/50 dilution.

Western blot - Anti-SLC51B antibody (AB121285)
  • WB

Supplier Data

Western blot - Anti-SLC51B antibody (AB121285)

All lanes:

Western blot - Anti-SLC51B antibody (ab121285) at 0.4 µg/mL

Lane 1:

Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate

Lane 2:

U-2 OS (Human bone osteosarcoma epithelial cell line) cell lysate

Predicted band size: 14 kDa

Observed band size: 14 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SLC51B aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q86UW2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC51B also known as OST-beta forms a heterodimer with SLC51A (OST-alpha) to function as an organic solute transporter involved in bile acid transport. This protein has a molecular mass of around 15 kDa. It is predominantly found in the ileum and liver key locations for bile acid circulation and metabolism. SLC51B localization to the basolateral membrane of enterocytes allows it to mediate transport across cellular barriers.
Biological function summary

SLC51B plays an important role in enterohepatic circulation by facilitating the transport of bile acids and steroids. It works as part of a transporter complex when combined with OST-alpha enhancing transport efficiency. This function helps in maintaining bile acid homeostasis and supports lipid digestion and absorption. SLC51B's activity assists in controlling cholesterol levels and prevents the accumulation of toxic bile acid concentrations within cells.

Pathways

The activity of SLC51B is integral to the enterohepatic bile acid recycling pathway and lipid metabolism pathways. It collaborates with proteins like NTCP and ASBT which are important for bile acid uptake in hepatocytes and enterocytes respectively. These pathways ensure efficient bile acid transport beginning from the liver its reabsorption in the intestines and return to the liver illustrating its central role in maintaining metabolic balance.

SLC51B plays a significant part in conditions like cholestasis and inflammatory bowel disease (IBD). Its role in bile acid transport links it to cholestasis where bile flow is reduced and its dysfunction can exacerbate the disease. Furthermore it associates with proteins such as FXR which regulates bile acid homeostasis and impacts inflammatory pathways contributing to the pathology of IBD. Understanding the relationship of SLC51B in these disorders highlights potential areas for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for bile acid export from enterocytes into portal blood (PubMed : 16317684). Modulates SLC51A glycosylation, membrane trafficking and stability activities (PubMed : 16317684). The Ost-alpha/Ost-beta complex efficiently transports the major species of bile acids (taurocholate) (PubMed : 16317684). Taurine conjugates are transported more efficiently across the basolateral membrane than glycine-conjugated bile acids (By similarity). Can also transport steroids such as estrone 3-sulfate and dehydroepiandrosterone 3-sulfate, therefore playing a role in the enterohepatic circulation of sterols (PubMed : 16317684). Able to transport eicosanoids such as prostaglandin E2 (By similarity).
See full target information SLC51B
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Computational and mathematical methods in medicine 2022:9076175 PubMed35592684

2022

Development of a Bile Acid-Related Gene Signature for Predicting Survival in Patients with Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Wang,Jun Guan,Qin Yang,Fengtian Wu,Junwei Shao,Qihui Zhou,Zixuan Guo,Yanli Ren,Haihong Zhu,Zhi Chen

Gastroenterology 159:1471-1486.e12 PubMed32553762

2020

Human Pluripotent Stem Cell-Derived Organoids as Models of Liver Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Nadzim Bin Ramli,Yee Siang Lim,Chwee Tat Koe,Deniz Demircioglu,Weiquan Tng,Kevin Andrew Uy Gonzales,Cheng Peow Tan,Iwona Szczerbinska,Hongqing Liang,Einsi Lynn Soe,Zhiping Lu,Chaiyaboot Ariyachet,Ka Man Yu,Shu Hui Koh,Lai Ping Yaw,Nur Halisah Binte Jumat,John Soon Yew Lim,Graham Wright,Asim Shabbir,Yock Young Dan,Huck-Hui Ng,Yun-Shen Chan

Journal of natural medicines 72:929-936 PubMed30043216

2018

Morin protects glucocorticoid-induced osteoporosis through regulating the mitogen-activated protein kinase signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chenguang Wang,Xin Wan,Yun Li,Huafeng Zhang,Lei Zhang
View all publications
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Product promise

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