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AB262934

Anti-SLC5A12 antibody

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Rabbit Polyclonal SLC5A12 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SLC5A12 aa 200-250.

View Alternative Names

SMCT2, SLC5A12, Sodium-coupled monocarboxylate transporter 2, Electroneutral sodium monocarboxylate cotransporter, Low-affinity sodium-lactate cotransporter, Solute carrier family 5 member 12

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC5A12 antibody (AB262934)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC5A12 antibody (AB262934)

Paraffin-embedded human Human lymph node tissue stained for SLC5A12 using ab262934 at 1/1000 dilution in immunohistochemical analysis. Negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC5A12 antibody (AB262934)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC5A12 antibody (AB262934)

Paraffin-embedded human skin tissue stained for SLC5A12 using ab262934 at 1/1000 dilution in immunohistochemical analysis. Negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC5A12 antibody (AB262934)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC5A12 antibody (AB262934)

Paraffin-embedded human small intestine tissue stained for SLC5A12 using ab262934 at 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC5A12 antibody (AB262934)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC5A12 antibody (AB262934)

Paraffin-embedded human kidney tissue stained for SLC5A12 using ab262934 at 1/1000 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SLC5A12 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

Q1EHB4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SLC5A12 protein also known as SMCT2 (Sodium-coupled monocarboxylate transporter 2) plays a role in the transport of lactate pyruvate and other monocarboxylates by coupling these with sodium ions. SLC5A12 has an approximate molecular weight of 75 kDa. It expresses in various tissues with significant presence in the kidney intestine and brain. This transporter allows for efficient functioning of metabolic processes by facilitating the uptake and distribution of key metabolites.
Biological function summary

The transport activity of SLC5A12 permits regulation of intracellular pH and osmotic balance. It is particularly important for the reabsorption of lactate in the kidney and its disposal in other tissues. Unlike some other monocarboxylate transporters SLC5A12 does not participate in a larger protein complex. However its function is important for maintaining lactate homeostasis influencing energy metabolism and cellular communication.

Pathways

SLC5A12 integrates into the metabolic pathways linked with glycolysis and gluconeogenesis. It shares associations with the key glycolytic transporter SLC16A1 (MCT1) which also mediates monocarboxylate transport. SLC5A12's role extends to controlling the lactate shuttle between tissues which is central to energy metabolism and signaling processes. This connection highlights its contribution to cellular energy homeostasis and metabolic flexibility.

SLC5A12 associates with conditions such as metabolic acidosis and certain types of cancer. In metabolic acidosis impaired function of SLC5A12 can lead to the accumulation of lactate and other metabolites affecting blood pH levels. In cancer altered expression of SLC5A12 may impact lactate dynamics contributing to the Warburg effect observed in tumors. The interaction with SLC16A1 lends further understanding of how changes in monocarboxylate transport can influence disease risk and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as an electroneutral and low-affinity sodium (Na(+))-dependent sodium-coupled solute transporter (PubMed : 17692818). Catalyzes the transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, nicotinate, propionate, butyrate and beta-D-hydroxybutyrate (By similarity). May be responsible for the first step of reabsorption of monocarboxylates from the lumen of the proximal tubule of the kidney and the small intestine. May play also a role in monocarboxylates transport in the retina (By similarity).
See full target information SLC5A12
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Product promise

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