Anti-SLC5A6 antibody [EPR30529-531]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC5A6 antibody [EPR30529-531] (AB325124)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling SLC5A6 with ab325124 at 1/500 (1.028 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human placenta (PMID : 10329687). The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC5A6 antibody [EPR30529-531] (AB325124)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling SLC5A6 with ab325124 at 1/500 (1.028 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human lung. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC5A6 antibody [EPR30529-531] (AB325124)

Immunohistochemical analysis of paraffin-embedded (A) Wild-type (human cervical adenocarcinoma epithelial cell) cell pellet and (B) SLC5A6 knockout Hela cell pellet labeling SLC5A6 with ab325124 at 1/500 (1.028 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) : Wild-type Hela cell pellet, no staining on (B) : SLC5A6 knockout HeLa cell pellet. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC5A6 antibody [EPR30529-531] (AB325124)

Immunohistochemical analysis of paraffin-embedded (A) Wild-type HEK-293T (human embryonic kidney epithelial cell) cell pellet and (B) SLC5A6 knockout HEK-293T cell pellet labeling SLC5A6 with ab325124 at 1/500 (1.028 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) : Wild-type HEK-293T cell pellet, no staining on (B) : SLC5A6 knockout HEK-293T cell pellet. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC5A6 antibody [EPR30529-531] (AB325124)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling SLC5A6 with ab325124 at 1/500 (1.028 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on blood vessels in human cerebrum (PMID : 25809983). The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SLC5A6 antibody [EPR30529-531] (AB325124)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized SLC5A6 KO HEK-293T (SLC5A6 knockout human embryonic kidney epithelial cell), ab266804; Wild type HEK-293T (human embryonic kidney epithelial cell), ab255449 cells labelling SLC5A6 with ab325124 at 1/500 (1.028 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing cytoplasmic and membranous staining in wild type HEK-293T cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).

• WB

Supplier Data

Western blot - Anti-SLC5A6 antibody [EPR30529-531] (AB325124)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab325124 was shown to bind specifically to SLC5A6. Target of interest was observed at 80 kDa in wild-type 293T cell lysates (lane 1) and wild-type HeLa cell lysates (lane 3), with no signal observed at this size in SLC5A6 knockout cell line (lane 2 and lane 4).

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-SLC5A6 antibody [EPR30529-531] (ab325124) at 1/1000 dilution

Lane 1:

Wild-type 293T (human embryonic kidney epithelial cell), whole cell lysate at 60 µg

Lane 2:

SLC5A6 knockout 293T whole cell lysate at 60 µg

Lane 3:

Wild-type HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 60 µg

Lane 4:

SLC5A6 knockout HeLa whole cell lysate at 60 µg

Lane 5:

SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 60 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa,36 kDa

false

Exposure time: 70s