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Rabbit Recombinant Monoclonal SLC6A14 antibody. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Western blot - Anti-SLC6A14 antibody [EPR27113-178] (AB316193), expandable thumbnail
  • Western blot - Anti-SLC6A14 antibody [EPR27113-178] (AB316193), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A14 antibody [EPR27113-178] (AB316193), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A14 antibody [EPR27113-178] (AB316193), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A14 antibody [EPR27113-178] (AB316193), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBFlow Cyt (Intra)ICC/IFIP
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Target data

Function

Amino acid transporter that plays an important role in the absorption of amino acids in the intestinal tract. Mediates the uptake of a broad range of neutral and cationic amino acids (with the exception of proline) in a Na(+)/Cl(-)-dependent manner (PubMed:10446133). Transports non-alpha-amino acids such as beta-alanine with low affinity, and has a higher affinity for dipolar and cationic amino acids such as leucine and lysine (PubMed:18599538). Can also transport carnitine, butirylcarnitine and propionylcarnitine coupled to the transmembrane gradients of Na(+) and Cl(-) (PubMed:17855766).

Alternative names

Sodium- and chloride-dependent neutral and basic amino acid transporter B(0+), Amino acid transporter ATB0+, Solute carrier family 6 member 14, SLC6A14

Recommended products

Rabbit Recombinant Monoclonal SLC6A14 antibody. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27113-178
Purification technique
Affinity purification Protein A
Specificity

We have observed a non-specific band at approximately 100KDa in human skeletal muscle by WB.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The SLC6A14 protein also known as the ATB0+ transporter is a sodium- and chloride-dependent transport protein that facilitates the uptake of a broad range of neutral and cationic amino acids. Weighing approximately 71 kDa it is encoded by the SLC6A14 gene. This transporter is expressed in various tissues including the lung placenta and pancreas with notable expression in the intestinal tract and certain areas of the brain. SLC6A14 plays an important role in maintaining cellular amino acid supply essential for protein synthesis and energy production.

Biological function summary

The transporter is involved in nutrient absorption and neurotransmitter regulation. While not part of a larger complex SLC6A14 coordinates the transport of multiple amino acids which contributes to maintaining amino acid homeostasis across cellular membranes. This function is important during periods of increased demand such as growth stress or tissue repair. Its ability to handle high-affinity transport in varied physiological environments highlights its unique versatility and importance.

Pathways

SLC6A14 is integral to amino acid and neurotransmitter transport pathways. It participates in systems involved in the transport and metabolism of amino acids like the mTOR signaling pathway. The protein also interfaces with pathways controlling energy balance and metabolic regulation often interacting with proteins like SLC6A19. These interactions allow the protein to contribute to maintaining balances essential for cellular functions and metabolic homeostasis.

Associated diseases and disorders

Mutations and altered expression of SLC6A14 correlate with conditions such as cystic fibrosis and colorectal cancer. In cystic fibrosis improper transporter function affects amino acid absorption complicating nutritional status. The protein's upregulation in certain cancers suggests a role in cell growth and survival mechanisms. The SLC6A14 transporter also exhibits functional connections with proteins like SLC7A9 affecting amino acid availability to cells under pathological conditions. These associations highlight its potential as a therapeutic target for related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-SLC6A14 antibody [EPR27113-178] (ab316193), expandable thumbnail

    Western blot - Anti-SLC6A14 antibody [EPR27113-178] (ab316193)

    Negative control: pancreas(PMID:10446133)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-SLC6A14 antibody [EPR27113-178] (ab316193) at 1/1000 dilution

    Lane 1: Human breast tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Human pancreas tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 75-85 kDa, 36 kDa

    Exposure time: 26s

  • Western blot - Anti-SLC6A14 antibody [EPR27113-178] (ab316193), expandable thumbnail

    Western blot - Anti-SLC6A14 antibody [EPR27113-178] (ab316193)

    Negative control: SK-BR-3(PMID:21771784)

    Low expression: HCT116(PMID: 32219372)

    Lane1-2 were freshly made and used for Western blotting immediately to minimize protein degradation.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lane1-2: 70 seconds, lane3-5: 180 seconds

    All lanes: Western blot - Anti-SLC6A14 antibody [EPR27113-178] (ab316193) at 1/1000 dilution

    Lanes 1 and 3: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 4: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 5: SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 75-85 kDa, 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A14 antibody [EPR27113-178] (ab316193), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A14 antibody [EPR27113-178] (ab316193)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling SLC6A14 with ab316193 at 1/200 (2.585 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Negative control: No staining on human cardiac muscle.

    The section was incubated with ab316193 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A14 antibody [EPR27113-178] (ab316193), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A14 antibody [EPR27113-178] (ab316193)

    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling SLC6A14 with ab316193 at 1/200 (2.585 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on human colon carcinoma (PMID: 35907820).

    The section was incubated with ab316193 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A14 antibody [EPR27113-178] (ab316193), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A14 antibody [EPR27113-178] (ab316193)

    Immunohistochemical analysis of paraffin-embedded Human salivary gland tissue labeling SLC6A14 with ab316193 at 1/200 (2.585 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on human salivary gland.

    The section was incubated with ab316193 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A14 antibody [EPR27113-178] (ab316193), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A14 antibody [EPR27113-178] (ab316193)

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling SLC6A14 with ab316193 at 1/200 (2.585 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on human breast.

    The section was incubated with ab316193 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Product protocols

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