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Rabbit Recombinant Monoclonal SLC6A14 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Western blot - Anti-SLC6A14 antibody [EPR27113-178] - BSA and Azide free (AB316194), expandable thumbnail
  • Western blot - Anti-SLC6A14 antibody [EPR27113-178] - BSA and Azide free (AB316194), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A14 antibody [EPR27113-178] - BSA and Azide free (AB316194), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A14 antibody [EPR27113-178] - BSA and Azide free (AB316194), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A14 antibody [EPR27113-178] - BSA and Azide free (AB316194), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBFlow Cyt (Intra)ICC/IFIP
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Target data

Function

Amino acid transporter that plays an important role in the absorption of amino acids in the intestinal tract. Mediates the uptake of a broad range of neutral and cationic amino acids (with the exception of proline) in a Na(+)/Cl(-)-dependent manner (PubMed:10446133). Transports non-alpha-amino acids such as beta-alanine with low affinity, and has a higher affinity for dipolar and cationic amino acids such as leucine and lysine (PubMed:18599538). Can also transport carnitine, butirylcarnitine and propionylcarnitine coupled to the transmembrane gradients of Na(+) and Cl(-) (PubMed:17855766).

Alternative names

Sodium- and chloride-dependent neutral and basic amino acid transporter B(0+), Amino acid transporter ATB0+, Solute carrier family 6 member 14, SLC6A14

Recommended products

Rabbit Recombinant Monoclonal SLC6A14 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27113-178
Purification technique
Affinity purification Protein A
Specificity

We have observed a non-specific band at approximately 100KDa in human skeletal muscle by WB.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab316194 is the carrirer-free version of Anti-SLC6A14 antibody [EPR27113-178] ab316193.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The SLC6A14 protein also known as the ATB0+ transporter is a sodium- and chloride-dependent transport protein that facilitates the uptake of a broad range of neutral and cationic amino acids. Weighing approximately 71 kDa it is encoded by the SLC6A14 gene. This transporter is expressed in various tissues including the lung placenta and pancreas with notable expression in the intestinal tract and certain areas of the brain. SLC6A14 plays an important role in maintaining cellular amino acid supply essential for protein synthesis and energy production.

Biological function summary

The transporter is involved in nutrient absorption and neurotransmitter regulation. While not part of a larger complex SLC6A14 coordinates the transport of multiple amino acids which contributes to maintaining amino acid homeostasis across cellular membranes. This function is important during periods of increased demand such as growth stress or tissue repair. Its ability to handle high-affinity transport in varied physiological environments highlights its unique versatility and importance.

Pathways

SLC6A14 is integral to amino acid and neurotransmitter transport pathways. It participates in systems involved in the transport and metabolism of amino acids like the mTOR signaling pathway. The protein also interfaces with pathways controlling energy balance and metabolic regulation often interacting with proteins like SLC6A19. These interactions allow the protein to contribute to maintaining balances essential for cellular functions and metabolic homeostasis.

Associated diseases and disorders

Mutations and altered expression of SLC6A14 correlate with conditions such as cystic fibrosis and colorectal cancer. In cystic fibrosis improper transporter function affects amino acid absorption complicating nutritional status. The protein's upregulation in certain cancers suggests a role in cell growth and survival mechanisms. The SLC6A14 transporter also exhibits functional connections with proteins like SLC7A9 affecting amino acid availability to cells under pathological conditions. These associations highlight its potential as a therapeutic target for related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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