Anti-SLC6A19 antibody [EPR30005-529]

• mIHC

Lab

Multiplex immunohistochemistry - Anti-SLC6A19 antibody [EPR30005-529] (AB326561)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue staining SLC6A19 with ab326561 at a 1 : 500 (0.996 µg/ml) dilution, ab322047 anti-GLEPP1/PTPRO used at 1 : 2000 (0.261 µg/ml) dilution and ab212197 anti-REN used at a 1 : 8000 (0.133 µg/ml) dilution.

Panel A : anti-SLC6A19 (green; Opal™520), anti-GLEPP1/PTPRO (magenta; Opal™690), anti-REN (gray; Opal™570) on human kidney.
Panel B : anti-SLC6A19 staining apical membrane of kidney tubules in human kidney.
Panel C : anti-GLEPP1/PTPRO staining glomerulus in human kidney.
Panel D : anti-REN staining juxtaglomerular cells in human kidney.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab326561, ab322047 and ab212197 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A19 antibody [EPR30005-529] (AB326561)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SLC6A19 with ab326561 at 1/500 (0.996 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : Weak staining in human colon.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-SLC6A19 antibody [EPR30005-529] (AB326561)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human small intestine tissue staining SLC6A19 with ab326561 at a 1 : 500 (0.996 µg/ml) dilution, ab272692 anti-MUC2 used at 1 : 2000 (0.26 µg/ml) dilution and ab325601 anti-Wnt3 used at a 1 : 2000 (0.246 µg/ml) dilution.

Panel A : anti-SLC6A19 (green; Opal™520), anti-MUC2 (magenta; Opal™690), anti-Wnt3 (gray; Opal™570) on human small intestine.
Panel B : anti-SLC6A19 staining apical membrane of epithelium in human small intestine.
Panel C : anti-MUC2 staining goblet cells in human small intestine.
Panel D : anti-Wnt3 staining Paneth cells in human small intestine.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab326561, ab272692 and ab325601 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A19 antibody [EPR30005-529] (AB326561)

Immunohistochemical analysis of paraffin-embedded Human duodenum tissue labeling SLC6A19 with ab326561 at 1/500 (0.996 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human duodenum.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A19 antibody [EPR30005-529] (AB326561)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SLC6A19 with ab326561 at 1/500 (0.996 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human kidney.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-SLC6A19 antibody [EPR30005-529] (AB326561)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse kidney tissue staining SLC6A19 with ab326561 at a 1 : 500 (0.996 µg/ml) dilution, ab322047 anti-GLEPP1/PTPRO used at 1 : 2000 (0.261 µg/ml) dilution and ab212197 anti-REN used at a 1 : 8000 (0.133 µg/ml) dilution.

Panel A : anti-SLC6A19 (green; Opal™520), anti-GLEPP1/PTPRO (magenta; Opal™690), anti-REN (gray; Opal™570) on mouse kidney.
Panel B : anti-SLC6A19 staining apical membrane of kidney tubules in mouse kidney.
Panel C : anti-GLEPP1/PTPRO staining glomerulus in mouse kidney.
Panel D : anti-REN staining juxtaglomerular cells in mouse kidney.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab326561, ab322047 and ab212197 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A19 antibody [EPR30005-529] (AB326561)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling SLC6A19 with ab326561 at 1/500 (0.996 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse kidney.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A19 antibody [EPR30005-529] (AB326561)

Immunohistochemical analysis of paraffin-embedded Mouse duodenum tissue labeling SLC6A19 with ab326561 at 1/500 (0.996 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse duodenum.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Lab

Western blot - Anti-SLC6A19 antibody [EPR30005-529] (AB326561)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : testis, colon (PMID : 15286787).

Lanes 1-4 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross reactivity with human IgG at 1/2000 and lane 5 is applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

Exposure time : Lane 1 : 6 seconds; Lanes 2-4 : 15 seconds.

All lanes:

Western blot - Anti-SLC6A19 antibody [EPR30005-529] (ab326561) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

Human small intestine tissue lysate at 20 µg

Lane 3:

Human testis tissue lysate at 20 µg

Lane 4:

Human colon tissue lysate at 20 µg

Lane 5:

JEG-3 (human placenta epithelial cell) whole cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Lane 5:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 71-90 kDa,36 kDa

false

• WB

Lab

Western blot - Anti-SLC6A19 antibody [EPR30005-529] (AB326561)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : testis, colon (PMID : 15286787).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-SLC6A19 antibody [EPR30005-529] (ab326561) at 1/1000 dilution

Lane 1:

Mouse kidney tissue lysate at 20 µg

Lane 2:

Mouse small intestine tissue lysate at 20 µg

Lane 3:

Mouse testis tissue lysate at 20 µg

Lane 4:

Mouse colon tissue lysate at 20 µg

Lane 5:

Rat kidney tissue lysate at 20 µg

Lane 6:

Rat small intestine tissue lysate at 20 µg

Lane 7:

Rat testis tissue lysate at 20 µg

Lane 8:

Rat colon tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 71-90 kDa,36 kDa

false

Exposure time: 37s

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A19 antibody [EPR30005-529] (AB326561)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling SLC6A19 with ab326561 at 1/500 (0.996 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining in mouse colon.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A19 antibody [EPR30005-529] (AB326561)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling SLC6A19 with ab326561 at 1/500 (0.996 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in mouse testis (PMID : 15286787).

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A19 antibody [EPR30005-529] (AB326561)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling SLC6A19 with ab326561 at 1/500 (0.996 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human testis.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• I-ELISA

Lab

Indirect ELISA - Anti-SLC6A19 antibody [EPR30005-529] (AB326561)

Indirect ELISA analysis of ab326561 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human SLC6A19 recombinant protein.

Antigen concentration : 1000 ng/ml