Rabbit Polyclonal SLC6A7 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SLC6A7 aa 100-250.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SLC6A7 aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q99884
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes Recommend PFA Fixation and Triton X-100 treatment |
Target data
Function
Brain specific sodium (and chloride)-dependent proline transporter (PubMed:7651355). Terminates the action of proline by its high affinity sodium-dependent reuptake into presynaptic terminals (Probable).
Alternative names
PROT, SLC6A7, Sodium-dependent proline transporter, Solute carrier family 6 member 7
Recommended products
Rabbit Polyclonal SLC6A7 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SLC6A7 aa 100-250.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SLC6A7 aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q99884
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SLC6A7 also known as the Proton-dependent Amino Acid Transporter (PAT2) is a member of the solute carrier family 6. This transporter specifically facilitates the uptake of proline and other amino acids in cells working through a sodium-dependent mechanism. It has a molecular mass of approximately 76 kDa. SLC6A7 is predominantly expressed in the central nervous system with notable expression in the brain highlighting its role in neural processes.
- Biological function summary
The transporter plays a significant role in neurotransmitter regulation and modulation. By controlling the levels of amino acids within neural tissues SLC6A7 contributes to neurotransmission and synaptic plasticity. This transporter functions as part of a broader amino acid transport system that maintains cellular homeostasis within neurons ensuring proper signaling and metabolic processes.
- Pathways
This solute carrier is an important component of amino acid transport and metabolism pathways. It participates in the neurotransmitter cycle interacting closely with GABA and glutamate pathways which are critical for synaptic signaling in the brain. SLC6A7 shares functional relationships with closely related transporters such as SLC6A1 and SLC6A3 which also partake in neurotransmitter processes and transport mechanisms.
- Associated diseases and disorders
SLC6A7 is linked to neurological conditions such as epilepsy and schizophrenia. The dysregulation of amino acid transport mediated by this protein can disrupt neurotransmitter balance which may contribute to the pathophysiology of these disorders. In schizophrenia for example altered function of SLC6A7 can have downstream effects on proteins like glutamate receptors impacting neuronal activity and cognitive function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-SLC6A7 antibody (ab122024)
Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm. Recommended concentration of ab122024 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A7 antibody (ab122024)
ab122024, at a 1/20 dilution, staining SLC6A7 in paraffin-embedded Human colon tissue, by Immunohistochemistry.
Downloads
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