JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB130132

Anti-SLC6A8 antibody

4

(1 Review)

|

(1 Publication)

Goat Polyclonal SLC6A8 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC6A8 aa 150-250.

View Alternative Names

Sodium- and chloride-dependent creatine transporter 1, CT1, Creatine transporter 1, Solute carrier family 6 member 8, SLC6A8

1 Images
Western blot - Anti-SLC6A8 antibody (AB130132)
  • WB

Supplier Data

Western blot - Anti-SLC6A8 antibody (AB130132)

All lanes:

Western blot - Anti-SLC6A8 antibody (ab130132) at 0.1 µg/mL

All lanes:

Mouse Heart lysate at 35 µg

Predicted band size: 70 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human SLC6A8 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

P48029

Specificity

ab130132 is expected to recognize all reported isoforms (NP_005620.1; NP_001136277.1; NP_001136278.1).

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC6A8 also known as the creatine transporter 1 (CRT1) is a protein that mediates the transport of creatine across the plasma membrane. It belongs to the solute carrier family 6 (SLC6) and has a mass of approximately 70 kDa. You can find SLC6A8 expressed widely in the brain skeletal muscles heart and kidneys where it plays a fundamental role in cellular energy homeostasis. The protein operates by utilizing the sodium and chloride gradients to facilitate creatine uptake which is critical for energy storage and supply in cells.
Biological function summary

This protein provides creatine for phosphate buffering and energy recycling in tissues with high-energy demands. SLC6A8 forms part of a complex that collaborates closely with creatine kinase enzymes enabling the maintenance of ATP levels in energy-intensive cells. Creatine uptake is essential for maintaining energy balance and SLC6A8 ensures that cells receive an adequate supply of creatine for their needs.

Pathways

SLC6A8 plays an integral role in the creatine biosynthesis and transport pathway. Its proper functioning is important for pathways involving energy metabolism particularly the phosphocreatine shuttle which connects it with creatine kinases. SLC6A8 is also part of the broader metabolic network interacting with other transporters and proteins involved in cellular energy dynamics ensuring efficient regulation of energy resources within cells.

Disruptions in SLC6A8 function can lead to conditions such as creatine transporter deficiency and X-linked creatine deficiency syndrome. These conditions often result in impaired cognitive development seizures and muscle weakness primarily due to insufficient creatine transport into the brain and muscles. In these disorders SLC6A8's interaction with the creatine kinase and other related enzymes proves significant emphasizing the importance of its proper function in avoiding such energy metabolism disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Creatine : sodium symporter which mediates the uptake of creatine (PubMed : 17465020, PubMed : 22644605, PubMed : 25861866, PubMed : 7945388, PubMed : 7953292, PubMed : 9882430). Plays an important role in supplying creatine to the brain via the blood-brain barrier (By similarity).
See full target information SLC6A8
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2309292 PubMed38666459

2024

Genetic Screening of Haploid Neural Stem Cells Reveals that Nfkbia and Atp2b4 are Key Regulators of Oxidative Stress in Neural Precursors.

Applications

Unspecified application

Species

Unspecified reactive species

Shaochen Nie,Wenhao Zhang,Xin Jin,Xiaoyan Li,Shengyi Sun,Yiding Zhao,Qingshen Jia,Luyuan Li,Yan Liu,Dayong Liu,Qian Gao
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB137198

Anti-SLC6A8 antibody

Western blot - Anti-SLC6A8 antibody (AB137198)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB52635

Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

  • 5
  • 10
Primary Antibodies

AB76013

Anti-TTF1/Nkx2-1 antibody [EP1584Y]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (AB76013)

  • 5
  • 17
Assay Kits

AB241028

L-Arginine Assay Kit

Enzyme activity assay - L-Arginine Assay Kit (AB241028)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com