AB203036

Anti-Slco1a1/Oatp1a1 antibody

Name: Anti-Slco1a1/Oatp1a1 antibody (ab203036) | Abcam
Brand: Abcam Limited
SKU: AB203036
Price: 510 USD
Availability: InStock

(2 Reviews)

(3 Publications)

Rabbit Polyclonal Slco1a1/Oatp1a1 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Rat Slco1a1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Oatp1, Oatp1a1, Slc21a1, Slco1a1, Solute carrier organic anion transporter family member 1A1, Organic anion-transporting polypeptide 1, Sodium-independent organic anion transporter 1, Solute carrier family 21 member 1, OATP-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slco1a1/Oatp1a1 antibody (AB203036)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slco1a1/Oatp1a1 antibody (AB203036)

Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse kidney tissue sections labeling Slco1a1/Oatp1a1 with ab203036 at 1/200 followed by conjugation to the secondary antibody and DAB staining.

Supplier Data

Western blot - Anti-Slco1a1/Oatp1a1 antibody (AB203036)

All lanes:

Western blot - Anti-Slco1a1/Oatp1a1 antibody (ab203036) at 1/200 dilution

Lane 1:

Mouse liver lysate

Lane 2:

Rat brain lysate

Secondary

All lanes:

HRP conjugated secondary at 1/3000 dilution

Predicted band size: 74 kDa

Observed band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Rat Slco1a1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P46720

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/500", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Slco1a1 also known as Oatp1a1 is a solute carrier protein that functions as a transporter. Its mass is approximately 80 kDa. This protein is significant for mediating the uptake of organic anions and diverse drugs across the cell membranes. It is mostly expressed in the liver with some presence in the kidneys and other tissues. As a member of the OATP (organic anion-transporting polypeptides) family it facilitates the transport of bile acids hormones and various xenobiotics.

Biological function summary

Slco1a1 plays an important role in detoxification and drug metabolism. It is not considered part of a multi-subunit complex but it contributes to cellular processes by modulating concentrations of its substrates within cells. Its substrate specificity allows it to impact the pharmacokinetics of certain drugs affecting their hepatic uptake and clearance. Consequently this protein can influence drug efficacy and potential toxicity.

Pathways

Slco1a1 is integral to the hepatic uptake pathway of xenobiotics and the enterohepatic recirculation of bile acids. It interacts with proteins such as the organic cation transporter 1 (OCT1) which manages the transportation of cations. This coordination assists in the broader regulation of bile acid biosynthesis and circulation which are vital to lipid digestion and cholesterol homeostasis.

Slco1a1 has connections to drug-induced liver injury and cholestasis. Altered expression or mutations in Slco1a1 can lead to impaired transport capacity increasing susceptibility to these conditions. The protein collaborates with cytochrome P450 enzymes which also play roles in drug metabolism and are implicated in liver pathologies. This highlights its importance in maintaining liver function and its potential as a therapeutic target.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Mediates the Na(+)-independent transport of organic anions such as steroid sulfate conjugates (dehydroepiandrosterone sulfate (DHEAS), 17-beta-glucuronosyl estradiol, estrone-3-sulfate), conjugated (taurocholate) and unconjugated (cholate) bile acids, prostaglandin E2 (PGE2) and L-thyroxine T4 (PubMed : 19129463). Also capable of transporting sulfobromophthalein (BSP), ouabain and gadoxetate (By similarity). Hydrogencarbonate/HCO3(-) acts as the probable counteranion that exchanges for organic anions (PubMed : 19129463). Shows a pH-sensitive substrate specificity which may be ascribed to the protonation state of the binding site and leads to a stimulation of substrate transport in an acidic microenvironment (PubMed : 19129463).

See full target information Slco1a1

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of the science of food and agriculture 103:1283-1293 PubMed36087337

2022

L-Theanine regulates lipid metabolism by modulating gut microbiota and bile acid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Xu,Yingying Kong,Tuo Zhang,Zhihua Gong,Wenjun Xiao

British journal of pharmacology 176:2848-2863 PubMed31077342

2019

Ursodeoxycholic acid accelerates bile acid enterohepatic circulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yunjing Zhang,Runqiu Jiang,Xiaojiao Zheng,Sha Lei,Fengjie Huang,Guoxiang Xie,Sandi Kwee,Herbert Yu,Christine Farrar,Beicheng Sun,Aihua Zhao,Wei Jia

Cells 8: PubMed30884843

2019

Ursodeoxycholic Acid Regulates Hepatic Energy Homeostasis and White Adipose Tissue Macrophages Polarization in Leptin-Deficiency Obese Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Sheng Chen,Hsuan-Miao Liu,Tzung-Yan Lee

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Slco1a1/Oatp1a1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slco1a1/Oatp1a1 antibody (AB203036)

Western blot - Anti-Slco1a1/Oatp1a1 antibody (AB203036)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise